A5102765440- Malaria Research and Control
- Insect Resistance and Genetics
- Drug Transport and Resistance Mechanisms
- Mosquito-borne diseases and control
- Insect Pest Control Strategies
- Viral Infectious Diseases and Gene Expression in Insects
- Evolution and Genetic Dynamics
- Toxic Organic Pollutants Impact
- Astro and Planetary Science
- Neuroscience and Neuropharmacology Research
- Spacecraft and Cryogenic Technologies
- Metabolomics and Mass Spectrometry Studies
- Neurological Disorders and Treatments
- Agricultural pest management studies
- Carcinogens and Genotoxicity Assessment
- Insect-Plant Interactions and Control
- HIV Research and Treatment
- Insect symbiosis and bacterial influences
- Cancer-related Molecular Pathways
- Cell Image Analysis Techniques
- Cancer Genomics and Diagnostics
- Plant Parasitism and Resistance
- Biological Control of Invasive Species
- Air Quality and Health Impacts
- Genetically Modified Organisms Research
Texas Biomedical Research Institute
2018-2022
University of Milan
2016-2019
Fitness costs are key determinants of whether drug resistance alleles establish and how fast they spread within populations. More than 125 different kelch13 alleles, each containing a amino acid substitution, have arisen in Southeast Asian malaria parasite (Plasmodium falciparum) populations under artemisinin selection over the past 15 years dramatic example soft selective event. However, just one these (C580Y) is now outcompeting other multiple countries spreading toward fixation. Here we...
Abstract Animals respond to chemical stress with an array of gene families and pathways termed “chemical defensome”. In arthropods, despite many defensome genes have been detected, how their activation is arranged during toxic exposure remains poorly understood. Here, we sequenced the transcriptome Anopheles stephensi larvae exposed for six, 24 48 hours LD 50 dose insecticide permethrin monitor transcriptional changes across time. A total 177 involved in defense were differentially expressed...
Insecticides remain a main tool for the control of arthropod vectors. The urgency to prevent insurgence insecticide resistance and perspective find new target sites, development novel molecules, are fuelling study molecular mechanisms involved in insect defence against xenobiotic compounds. In this study, we have investigated if ATP-binding cassette (ABC) transporters, major component defensome machinery, permethrin, susceptible larvae malaria vector Anopheles gambiae sensu stricto....
Drug resistance mutations tend to disrupt key physiological processes and frequently carry fitness costs, which are a central determinant of the rate spread these in natural populations. Head-to-head competition assays provide standard approach measuring for malaria parasites. These typically use standardized culture medium containing RPMI 1640, has 1.4- 5.5-fold higher concentration amino acids than human blood. In this rich medium, we predict that costs will be underestimated because...
Insecticides are still at the core of insect pest and vector control programmes. Several lines evidence indicate that ABC transporters involved in detoxification processes against insecticides, including permethrin other pyrethroids. In particular, ABCG4 gene, a member G subfamily, has consistently been shown to be up-regulated response insecticide treatments mosquito malaria Anopheles stephensi (both adults larvae).To verify actual involvement this transmembrane protein process permethrin,...
Malaria parasites break down host hemoglobin into peptides and amino acids in the digestive vacuole for export to parasite cytoplasm growth: interrupting this process is central mode of action several antimalarial drugs. Mutations chloroquine (CQ) resistance transporter, pfcrt , located membrane, confer CQ Plasmodium falciparum but typically affect fitness. However, role other loci evolution unclear. Here we use a combination population genomics, genetic crosses gene editing demonstrate that...
ABSTRACT Detoxifying pathways of mosquitoes against the neem (Azadirachta indica) extracts are still unclear. The aim present study was to investigate role adenosine triphosphate–binding cassette (ABC) transporters in this process Anopheles stephensi, one main malaria vectors southern Asia. Third-stage larvae An. stephensi were fed with fish food alone or combination extract at 0.5%, 1%, 5%, and 10%. Six ABC-transporter genes from 3 different subfamilies (B, C, G) analyzed assess their...
In the adult central nervous system GABA mediates fast inhibitory transmission, whereas during development it is an excitatory transmitter and a trophic factor involved in controlling morphogenesis (Ben-Ari, 2002). This funcional shift occurs as result of progressively increasing expression KCC2 cotransporter, major chloride extruder mature neurons. several pathological conditions associated with hyperexcitability, such epilepsy, suppression may contribute to alter balance excitation...
Summary Drug resistance mutations tend to disrupt key physiological processes, and therefore carry a fitness cost. The size of these costs is central determinant the rate spread in natural populations so are important quantify. Head-to-head competition assays provide standard approach measuring differential fitness, have been used extensively for malaria parasites. These typically use standardized culture media, containing RPMI 1640, which has 1.4 5.5-fold (mean: 2.6-fold) higher...