A5080683966- Blood properties and coagulation
- Cancer Immunotherapy and Biomarkers
- Erythrocyte Function and Pathophysiology
- Mitochondrial Function and Pathology
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Platelet Disorders and Treatments
- Venous Thromboembolism Diagnosis and Management
- SARS-CoV-2 and COVID-19 Research
- Metabolism and Genetic Disorders
- Adipokines, Inflammation, and Metabolic Diseases
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 detection and testing
- Blood Coagulation and Thrombosis Mechanisms
- Erythropoietin and Anemia Treatment
- Advanced biosensing and bioanalysis techniques
- Peptidase Inhibition and Analysis
- Biotin and Related Studies
- Biosensors and Analytical Detection
- RNA modifications and cancer
- Extracellular vesicles in disease
- Click Chemistry and Applications
- Cellular transport and secretion
- Lipoproteins and Cardiovascular Health
- Cancer Mechanisms and Therapy
University of California, San Francisco
2020-2025
University of North Carolina at Chapel Hill
2013-2023
Children's Hospital of Philadelphia
2015-2019
Indiana University School of Medicine
2017
Stony Brook University
2010-2015
Medical College of Wisconsin
2013
University of St. Thomas - Minnesota
2012
Cold Spring Harbor Laboratory
2009-2011
Moffitt Cancer Center
1995
Oklahoma Medical Research Foundation
1995
Venous thrombi, fibrin- and rbc-rich clots triggered by inflammation blood stasis, underlie devastating, sometimes fatal, occlusive events. During intravascular fibrin deposition, rbc are thought to become passively trapped in thrombi therefore have not been considered a modifiable thrombus component. In the present study, we determined that activity of transglutaminase factor XIII (FXIII) is critical for retention within directly affects size. Compared with WT mice, mice carrying homozygous...
Abstract Targeted degradation of cell surface and extracellular proteins via lysosomal delivery is an important means to modulate biology. However, these approaches have limitations due lack modularity, ease development, restricted tissue targeting applicability both proteins. We describe a strategy, termed cytokine receptor-targeting chimeras (KineTACs), that addresses limitations. KineTACs are fully genetically encoded bispecific antibodies consisting arm, which binds its cognate receptor,...
Comprehensive understanding of the serological response to SARS-CoV-2 infection is important for both pathophysiologic insight and diagnostic development. Here, we generate a pan-human coronavirus programmable phage display assay perform proteome-wide profiling antigens enriched by 98 COVID-19 patient sera. Next, use ReScan, method efficiently sequester expressing most immunogenic peptides print them onto paper-based microarrays using acoustic liquid handling, which isolates identifies nine...
As severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to spread around the world, there is an urgent need for new assay formats characterize humoral response infection. Here, we present efficient, competitive serological that can simultaneously determine individual's seroreactivity against SARS-CoV-2 Spike protein and proportion of anti-Spike antibodies block interaction with human angiotensin-converting enzyme (ACE2) required viral entry. In this approach based on use...
The identification of genetic and epigenetic alterations from primary tumor cells has become a common method to identify genes critical the development progression cancer. We seek those aberrations that have most impact on gene function within tumor. First, we perform bioinformatic analysis copy number variation (CNV) DNA methylation covering landscape ovarian cancer cells. separately examined CNV for 42 serous samples using MOMA-ROMA assays 379 analyzed by Cancer Genome Atlas. identified...
Characterization of cell surface proteome differences between cancer and healthy cells is a valuable approach for the identification novel diagnostic therapeutic targets. However, selective sampling proteins proteomics requires large samples (>10e6 cells) long labeling times. These limitations preclude analysis material-limited biological or capture rapid proteomic changes. Here, we present two approaches to tether exogenous peroxidases (APEX2 HRP) directly cells, enabling rapid, small-scale...
N terminomics is a powerful strategy for profiling proteolytic neo-N termini, but its application to cell surface proteolysis has been limited by the low relative abundance of plasma membrane proteins. Here we apply membrane-targeted subtiligase variants (subtiligase-TM) efficiently and specifically capture termini in live cells. Using this approach, sequenced 807 quantified changes their response stimuli that induce remodeling proteome. To facilitate exploration our datasets, developed...
Objective— Individuals with elevated prothrombin, including those the prothrombin G20210A mutation, have increased risk of venous thrombosis. Although these individuals do not circulating prothrombotic biomarkers, their plasma demonstrates tissue factor–dependent thrombin generation in vitro. The objectives this study were to determine pathological role and arterial thrombosis vivo, distinguish thrombogenic mechanisms vessels. Approach Results— Prothrombin was infused into mice raise levels....