A5002662771- Memory and Neural Mechanisms
- Neuroscience and Neuropharmacology Research
- Sleep and Wakefulness Research
- Neural dynamics and brain function
- Retinoids in leukemia and cellular processes
- Stress Responses and Cortisol
- Nuclear Receptors and Signaling
- Adipose Tissue and Metabolism
- Advanced Fluorescence Microscopy Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Memory Processes and Influences
- Cell Image Analysis Techniques
- Photoreceptor and optogenetics research
- Anesthesia and Neurotoxicity Research
- Protein Degradation and Inhibitors
- Sleep and related disorders
- Advanced MRI Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Functional Brain Connectivity Studies
- Regulation of Appetite and Obesity
- Intensive Care Unit Cognitive Disorders
- RNA Research and Splicing
- Identity, Memory, and Therapy
- Genetics and Neurodevelopmental Disorders
- Biochemical Analysis and Sensing Techniques
University of Toyama
2016-2025
Japan Science and Technology Agency
2010-2025
Tokyo University of Agriculture
2008-2012
Unrelated memories get blurred together If one retrieves two around the same time, a small number of neurons will become involved in both memories. Yokose et al. investigated cellular ensemble mechanisms underlying association between such In mice, population mediates association. Memory traces for independent emotional brain partially overlapped when were retrieved synchronously to create linkage. Suppressing activity overlapping memory trace interrupted linkage without damaging original...
Previous studies have suggested that calcium/calmodulin-dependent protein kinase IV (CaMKIV) functions as a positive regulator for memory formation and age-related deficits are the result of dysfunctional signaling pathways mediated by cAMP response element-binding (CREB), downstream transcription factor CaMKIV. Little is known, however, about effects increased CaMKIV levels on ability to form in adult aged stages. We generated transgenic mouse overexpressing forebrain showed upregulation...
Abstract Background Retinoid signaling pathways mediated by retinoic acid receptor (RAR)/retinoid × (RXR)-mediated transcription play critical roles in hippocampal synaptic plasticity. Furthermore, recent studies have shown that treatment with alleviates age-related deficits long-term potentiation (LTP) and memory performance and, furthermore, a transgenic mouse model of Alzheimer's disease. However, the RAR/RXR pathway learning at behavioral level still not been well characterized adult...
Abstract Behavioural tagging is the transformation of a short-term memory, induced by weak experience, into long-term memory (LTM) due to temporal association with novel experience. The mechanism which neuronal ensembles, each carrying engram one experiences, interact achieve behavioural unknown. Here we show that retrieval LTM formed experience depends on degree overlap ensemble corresponding numbers neurons activated training in object recognition (NOR) task and context exploration (NCE)...
Idling brain activity has been proposed to facilitate inference, insight, and innovative problem-solving. However, it remains unclear how when the idling can create novel ideas. Here, we show that cortical offline is both necessary sufficient for building unlearned inferential knowledge from previously acquired information. In a transitive inference paradigm, male C57BL/6J mice gained 1 day after, but not shortly complete training. Inhibiting neuronal computations in anterior cingulate...
Previous gain-of-function studies using an optogenetic technique showed that manipulation of the hippocampal dentate gyrus or CA1 cell ensembles is important for memory reactivation and to generate synthetic false memory. However, study manipulating CA3 has not been reported. The area hippocampus comprises a recurrent excitatory circuit, which thought be generation associations among stored information within one brain region. We investigated whether coincident firing in region, CA3,...
Passive priming of prior knowledge to assimilate ongoing experiences underlies advanced cognitive processing. However, the necessary neural dynamics memory assimilation remains elusive. Uninstructed brain could also show boosted creativity, particularly after idling states, yet it unclear whether can spontaneously spark relevant assimilations. We established a paradigm that links/separates context-dependent memories according geometrical similarities. Mice exploring one four contexts 1 d...
The prefrontal cortex plays a crucial role in procedural rule learning; however, the specific neuronal mechanism through which it represents rules is unknown. We hypothesized that sequential activities encode these rules. To investigate this, we recorded medial of mice during learning using Ca 2+ imaging. utilized method based on convolutional negative matrix factorization, iSeq, to automatically detect temporal sequences data. As advanced, began critical information for execution. In had...
Cognitive processes such as action planning and decision-making require the integration of multiple sensory modalities in response to temporal cues, yet underlying mechanism is not fully understood. Sleep has a crucial role for memory consolidation promoting cognitive flexibility. Our aim identify sleep integrating different enhance flexibility task execution while identifying specific brain regions that mediate this process. We have designed "Auditory-Gated Patience-to-Action" Task which...
Active recall of short-term memory (STM) is known to last for a few hours, but whether STM has long-term functions unknown. Here we show that can be optogenetically retrieved at time point during which natural not possible, uncovering the existence an engram. Moreover, re-training within 3 days led recall, indicating facilitated consolidation. Inhibiting offline CA1 activity non-rapid eye movement (NREM) sleep, N-methyl-D-aspartate receptor (NMDAR) activity, or protein synthesis after first...
SUMMARY It has become possible to observe neural activity in freely moving animals via calcium imaging using a microscope, which could not be observed previously. However, it remains difficult extract the dynamics of nerve cells from recorded data. In this study, we greatly improved stability, and robustness cell estimation method non-negative matrix decomposition with shrinkage baseline. addition, by improving initial state iterative algorithm newly proposed shape image processing, solution...
Abstract The hippocampus must be capable of sorting and integrating multiple sensory inputs separately but simultaneously. However, it remains to elucidated how the executes these processes simultaneously during learning. Here we found that synchrony between conditioned stimulus (CS)-, unconditioned (US)- future retrieval-responsible cells occurs in CA1 reverberatory phase emerges after have ceased, not CS US inputs. Mutant mice lacking N-methyl-D-aspartate receptors (NRs) CA3 showed a...
Abstract The N -methyl- d -aspartate receptors (NRs) in hippocampal CA3 are crucial for the synaptic transmission and plasticity within recurrent circuit, which supports functions, such as pattern completion, reverberatory association of sensory inputs. Previous study showed that synchronous activation distinct cell populations CA3, correspond to events, associated independent suggesting circuit expressing NRs mediates artificial memory events stored ensembles. However, it is still unclear...
Abstract Currently, calcium imaging allows long-term recording of large-scale neuronal activity in diverse states. However, it remains difficult to extract dynamics from recorded data. In this study, we propose an improved constrained nonnegative matrix factorization (CNMF)-based algorithm and effective method cell shapes with fewer false positives negatives through image processing. We also show that the evaluation metrics obtained during signal processing can be combined used for...
Abstract Passive priming of prior knowledge to assimilate ongoing experiences underlies advanced cognitive processing. However, the necessary neural dynamics memory assimilation remains elusive. Uninstructed brain could also show boosted creativity, particularly after idling states, yet it unclear whether can spontaneously spark relevant assimilations. We established a paradigm that links/separates context-dependent memories according geometrical similarities. Mice exploring one four...
ABSTRACT Idling brain activity has been proposed to facilitate inference, insight, and innovative problem-solving 1-4 . However, it remains unclear how when the subconscious can create novel ideas. Here, we show coordinated roles of non-rapid eye movement (NREM) rapid-eye (REM) sleep in weaving inferential information from learned information. In a transitive inference paradigm, mice gained one day, but not shortly, after complete training. Inhibiting neuronal computations anterior cingulate...
Abstract Active recall of short-term memory (STM) is known to last for a few hours, but whether STM has long-term functions unknown. Here we show that, can be optogenetically retrieved at time point during which natural not possible, uncovering the existence silent engram. Moreover, re-training within 3 days led recall, indicating facilitated consolidation. Calcium imaging revealed hippocampal CA1 reactivations trace post-learning sleep. Inhibiting offline activity, N-methyl-D-aspartate...
Abstract Every day, we experience new daily episodes and store memories. Although memories are stored in corresponding engram cells, how different sets of cells selected for current next episodes, they create their memories, remains unclear. We report that mice, hippocampal CA1 neurons show an organized synchronous activity prelearning home cage sleep correlates with the learning ensembles only termed preconfigured ensembles. Moreover, after learning, a subset nonengram develops population...
ABSTRACT General anesthesia could induce amnesia, however the mechanism remains unclear. We hypothesized that suppression of neuronal ensemble activity in hippocampus by during post-learning period causes retrograde amnesia. To test this hypothesis, two experiments were conducted with sevoflurane (2.5%, 30 min): a hippocampus-dependent memory task, context pre-exposure facilitation effect (CPFE) procedure to measure function and vivo calcium imaging observe neural hippocampal CA1 exploration...
In entorhinal-hippocampal networks, the trisynaptic pathway, including CA3 recurrent circuit, processes episodes of context and space 1-3 . Recurrent connectivity can generate reverberatory activity 4-6 , an intrinsic pattern neurons that occurs after sensory inputs have ceased. However, role in memory encoding remains incompletely understood. Here we demonstrate mice, synchrony between conditioned stimulus (CS) unconditioned (US)-responsible cells during phase, lasting for approximately 15...