A5074461808- Enzyme Structure and Function
- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Stellar, planetary, and galactic studies
- Astrophysics and Star Formation Studies
- Monoclonal and Polyclonal Antibodies Research
- Molecular Spectroscopy and Structure
- Astronomy and Astrophysical Research
- Crystallization and Solubility Studies
- Plant and Fungal Interactions Research
- Enzyme Production and Characterization
- RNA and protein synthesis mechanisms
- Glycosylation and Glycoproteins Research
- Protein purification and stability
- Atmospheric Ozone and Climate
- Astronomical Observations and Instrumentation
- Protein Structure and Dynamics
- Biotin and Related Studies
- Insect and Arachnid Ecology and Behavior
- Plant Disease Resistance and Genetics
- Ziziphus Jujuba Studies and Applications
- Biofield Effects and Biophysics
- Astro and Planetary Science
- Biochemical and Structural Characterization
- Transgenic Plants and Applications
University of Groningen
2015-2019
Astronomical Institute of the Slovak Academy of Sciences
2018
University of Canterbury
2013-2015
University of California, Irvine
2000-2005
University of California, Riverside
1991-1998
Irvine University
1998
Anti-idiotypic monoclonal antibody 409.5.3 is raised against an that neutralizes feline infectious peritonitis virus. This antibody, used as immunogen, elicits the production of anti-anti-idiotypic antibodies in turn neutralize The crystal structure complex between anti-idiotypic Fab and idiotypic fragment virus-neutralizing has been solved by molecular replacement using real-space Patterson search filtering correlation-coefficient refinement. refined to R value 0.21 based on 21,310 unique...
Three crystal forms of the sweet-tasting protein thaumatin from African berry Thaumatococcus daniellii have been grown. These include two naturally occurring isoforms, A and B, that differ by a single amino acid, recombinant form isoform B expressed in yeast. The crystals are space groups C2 with = 117.7, b 44.9, c 38.0 A, beta 94.0 degrees, P2(1)2(1)2(1) 44.3, 63.7 72.7 tetragonal P4(1)2(1)2 58.6 151.8 A. structures all three solved molecular replacement subsequently refined to R factors...
The properties of disks around brown dwarfs and very low mass stars (hereafter VLMOs) provide important boundary conditions on the process planet formation inform us about numbers masses planets than can form in this regime. We use Herschel Space Observatory PACS spectrometer to measure continuum [O i] 63 μm line emission toward 11 VLMOs with known Taurus Chamaeleon I star-forming regions. fit radiative transfer models spectral energy distributions these sources. Additionally, we carry out a...
The protein thaumatin was studied as a model macromolecule for crystallization in microgravity-environment experiments conducted on two US Space Shuttle missions (USML-2 and LMS). In this investigation, we have evaluated compared the quality of space- earth-grown crystals using X-ray diffraction analyses, characterized them according to crystal size, resolution limit mosaicity. Two different approaches growing microgravity environment, dialysis liquid-liquid diffusion, were employed joint...
In this paper, we couple together the dust evolution code two-pop-py with thermochemical disk modelling ProDiMo. We create a series of models that simulate over time from 0.018 Myr to 10 Myr, including radial drift, growth, and settling grains. examine effects on mid-infrared gas emission, focussing spectral lines C2H2, CO2, HCN, NH3, OH, H2O are readily observable Spitzer upcoming E-ELT JWST. The addition acts increase line fluxes by reducing population small find all species except C2H2...
The physical properties of brown dwarf discs, in terms their shapes and sizes, are still largely unexplored by observations. To what extent discs similar to scaled-down T Tauri is currently unknown, this work a step towards establishing relationship through the eventual modelling future We use observations disc $\rho$ Oph 102 infer fiducial model around which we build small grid models, order CO, HCN, HCO+ line fluxes chemistry drives abundances. These first models be published relate...
The crystal structure of anti-idiotopic Fab 409.5.3, made against an E2 specific feline infectious peritonitis virus-neutralizing antibody 730.1.4, has been determined in its free form, at 2.9 Å resolution by molecular replacement. This antibody, used as immunogen, elicits the production anti-anti-idiotypic antibodies that turn neutralize virus. uncomplexed was refined using constrained-restrained least squares minimization and simulated annealing combination with conjugate gradient...
Canavalin, the major reserve protein of jack bean, was obtained in four different crystal forms. From structure determined by multiple isomorphous replacement a hexagonal unit cell, structures three other crystals were molecular replacement. In two cases, rhombohedral and cubic crystals, placement facilitated coincidence threefold symmetry with crystallographic operators. orthorhombic canavalin trimer asymmetric unit. The rhombohedral, subsequently refined using combination several...
The major extracellular protease from the fungus Pencillium cyclopium was crystallized in presence of p-phenylmethanesulfonyl fluoride (PMSF) and investigated by X-ray diffraction analysis. It subsequently cloned amino acid sequence deduced its cDNA. Although is only 49% identical to that proteinase K Tritirachium album, three-dimensional structures two proteases are virtually identical. model for P. refined simulated annealing an R 18% at 1.7 A resolution. greatest variation polypeptide...
The cationic isozyme of peroxidase isolated from suspension cultures peanut cells is a heme-containing and calcium-dependent glycoprotein having four covalently attached oligosaccharide chains. Attempts were made to crystallize the for X-ray diffraction analysis, these have met with some success. Crystals now been grown that are suitable full three-dimensional structural analysis. crystals thin plates we shown them be orthorhombic space group P2(1)2(1)2(1) = 48.1, b 97.2, c 146.2 A. diffract...