The aim of this study is to formulate and compare the physicochemical properties negatively charged liposomes poly(lactide-co-glycolide) (PLGA) nanoparticles loaded with gemcitabine hydrochloride. influence formulation variables on liposome nanoparticle particle size, zeta potential, encapsulation efficiency, drug release was evaluated. Although PEGylated were same size (∼200 nm), efficiency 1.4 times higher for than nanoparticles. optimized had 26.1 ± 0.18 18.8 1.52% respectively. from...

Objectives: The xCELLigence System Real-Time Cell Analyzer is used for label-free and real-time monitoring of cell proliferation, cytotoxicity, migration. This system based on recording the electronic impedance specially designed plates containing interdigitated gold microelectrodes. Here, we present how to optimize proliferation evaluate cytotoxicity by using system.

Abstract Recently drug‐induced senescence has gained momentum as a new approach in cancer therapy. It is accepted that senescent cells display typical phenotypic features including flattened, enlarged, and multinucleated cell morphology. However, it not well elucidated how these morphological alterations occur. The current study evaluates the possible role of Rho/Rho kinase pathway cardiac glycoside‐induced morphology HeLa cells. Our results indicate administration glycosides, ouabain,...

Extracellular-signal regulated kinase (ERK) signaling is required for a multitude of physiological and patho-physiological processes. However, the identities proteins that ERK phosphorylates to elicit these responses are incompletely known. Using an affinity purification methodology general utility, here we identify cytoplasmic dynein intermediate chain 2 (DYNC1I-2, IC-2) as novel substrate following epidermal growth factor receptor stimulation fibroblasts. IC-2 subunit dynein, minus-end...

A 50-year-old woman who had surgery 4 years earlier for papillary carcinoma of the thyroid received 30 mCi I-131 ablation. Follow-up imaging showed successful gland ablation; however, persistent activity was present in mediastinum corresponding to esophageal projection. The patient been experiencing dysphagia approximately 10 years. Esophageal scintigraphy delayed transit time. contrast esophagogram a rat tail deformity with narrowing most marked at gastroesophageal junction and dilatation...

In drug discovery, most small molecules cannot cross many stages, only a few can become candidates. Once the molecule is approved and marketed, nontarget effects that are not easily distinguishable from actual target of drugs might be evaluated. This situation restricts treatment. Thus, discovery new very long expensive process. recent years, without developing drugs, approach using different in indications apart licensed has gained importance. this study, connectivity map program, it was...

The aim of this study was to evaluate the effect finishing and polishing procedures compomer bulk-fill composite resins on cytotoxicity against human gingival fibroblasts by xCELLigence analysis.Filtek™ Bulk Fill Dyract XP were used. After curing, specimens randomly divided into two groups finishing-polishing applied one group; no other group. For first time in study, pure gold samples prepared with same weight base area as test wells containing determined control system used assess response...

Cancer takes the third place in cause of death worldwide after cardiovascular and infectious disease. Benzimidazole derivatives have demonstrated as potential new therapeutics for treatment cancer vitro vivo. In this study order to develop more potent anticancer agents, 27 novel benzimidazole been designed synthesized evaluate their cytotoxic activity MCF‐7 cells. Cytotoxic effects compounds were evaluated using MTT assay most active ones further studied determine mechanism activity. Four...

Rho kinase (ROCK), an enzyme belonging to the serine-threonine family, is involved in regulation of basic cellular processes such as morphology, movement, division, differentiation and apoptosis. On other hand, excessively activated ROCK can cause cardiovascular neurological disorders or cancer. In recent years, overactivation kinases has been associated with increased metastasis various tumor types explored target for development new anticancer drugs. We report here design synthesis five...

Abstract Objectives In cancer treatment, it is important to prevent or slow down metastasis as well preventing the proliferation of cells. this study, we aimed find pyrazole compounds with antimigratory properties. Methods The ‘PASSonline’ programme was used determine possible pharmacological activities selected from library, and two derivatives were identified a transcription factor STAT inhibitor high probability. There are studies known that JAK/STAT pathway related cell migration, thus...

Objectives: The aim of this study is to investigate the association treatment adherence, quality life and insight among bipolar disorder schizophrenia patients who are in remission reveal which variables predict adherence. Methods: We conducted a cross sectional with 150 remission. were administered Mini International Neuropsychiatric Interview (MINI), Brief Psychiatric Rating Scale (BPRS), Schedule for Assessing Three Components Insight (SAI), World Health Organization Questionnaire on...

Amaç: Bu çalışmada remisyondaki şizofreni ve bipolar bozukluk hastalarında depo antipsikotik kullanmanın etkinliği güvenirliğinin sosyodemografik özellikler, ilaç yan etkileri, hastalık şiddeti, yaşam kalitesi diğer klinik değişkenler açısından incelenmesi amaçlanmıştır.Gereç Yöntem: Psikiyatri polikliniğinde izlenen DSM-IV TR ölçütlerine göre tip I tanısı konan çalışmaya katılım ölçütlerini karşılayan, remisyon dönemindeki 100 bozukluk, hastası ardışık olarak alınmıştır. Şizofreni grubu...