A5090072672- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- Histone Deacetylase Inhibitors Research
- Protein Degradation and Inhibitors
- CAR-T cell therapy research
- Cancer Research and Treatments
- Adenosine and Purinergic Signaling
- Macrophage Migration Inhibitory Factor
- Neuroblastoma Research and Treatments
- Immune Cell Function and Interaction
- Pancreatic and Hepatic Oncology Research
- Biosimilars and Bioanalytical Methods
- Click Chemistry and Applications
- CRISPR and Genetic Engineering
- Chromatin Remodeling and Cancer
- Multiple Myeloma Research and Treatments
- Atherosclerosis and Cardiovascular Diseases
- Genomics and Chromatin Dynamics
- Ferroptosis and cancer prognosis
- Ubiquitin and proteasome pathways
Virginia Commonwealth University
2014-2018
Genetic studies in fruit flies have implicated the chromatin remodeling complex nucleosome factor (NURF) immunity, but it has yet to be studied mammals. Here we show that its targeting mice enhances antitumor immunity two syngeneic models of cancer. NURF was disabled by silencing bromodomain PHD-finger containing transcription (BPTF), largest and essential subunit NURF. We found both CD8+ CD4+ T cells were necessary for enhanced activity, with elevated numbers activated observed...
Using syngeneic BALB/c mouse breast cancer models, we show that the chromatin remodeling subunit bromodomain PHD finger transcription factor (BPTF) suppresses natural killer (NK) cell antitumor activity in tumor microenvironment (TME). In culture, BPTF direct cytotoxicity receptor (NCR) mediated NK cytolytic to and human lines, demonstrating conserved functions. Blocking NCR1 vivo rescues KD weights, its importance for control of growth. We discovered occupies heparanase (Hpse) regulatory...
<p>Gene Ontology of microarray data by DAVID analysis.</p>
<p>BPTF does not affect 4T1 cellular morphology or proliferation in vitro.</p>
<p>Enhanced CD8 T cell cytotoxicity to BPTF KD cells is not due the OVA or pmel antigens.</p>
<p>BPTF depleted 4T1 tumors are flat compared to controls and BPTF does not affect MDSC amplification or metastasis the lung.</p>
<p>Supplemental figure legends for Mayes et al.</p>
<p>BPTF does not affect B16F10 cellular morphology or proliferation in vitro but BPTF depleted tumors are flat compared to controls.</p>
<div>Abstract<p>Genetic studies in fruit flies have implicated the chromatin remodeling complex nucleosome factor (NURF) immunity, but it has yet to be studied mammals. Here we show that its targeting mice enhances antitumor immunity two syngeneic models of cancer. NURF was disabled by silencing bromodomain PHD-finger containing transcription (BPTF), largest and essential subunit NURF. We found both CD8<sup>+</sup> CD4<sup>+</sup> T cells were necessary...
<p>Microarray data analysis of significant genes in 4T1 tumors harvested from NSG mice. List primer sequences used this study.</p>
<p>Flow cytometry antibodies are specific.</p>
<p>BPTF KD does not induce an interferon type I response and BPTF occupy features other than promoters.</p>
<div>Abstract<p>Genetic studies in fruit flies have implicated the chromatin remodeling complex nucleosome factor (NURF) immunity, but it has yet to be studied mammals. Here we show that its targeting mice enhances antitumor immunity two syngeneic models of cancer. NURF was disabled by silencing bromodomain PHD-finger containing transcription (BPTF), largest and essential subunit NURF. We found both CD8<sup>+</sup> CD4<sup>+</sup> T cells were necessary...
<p>BPTF depleted 4T1 tumors are flat compared to controls and BPTF does not affect MDSC amplification or metastasis the lung.</p>
<p>Enhanced CD8 T cell cytotoxicity to BPTF KD cells is not due the OVA or pmel antigens.</p>
<p>Microarray data analysis of significant genes in 4T1 tumors harvested from NSG mice. List primer sequences used this study.</p>
<p>Supplemental figure legends for Mayes et al.</p>
<p>BPTF does not affect 4T1 cellular morphology or proliferation in vitro.</p>
<p>BPTF KD does not induce an interferon type I response and BPTF occupy features other than promoters.</p>
<p>Gene Ontology of microarray data by DAVID analysis.</p>
<p>BPTF does not affect B16F10 cellular morphology or proliferation in vitro but BPTF depleted tumors are flat compared to controls.</p>
<p>Flow cytometry antibodies are specific.</p>
Abstract Using syngeneic BALB/c mouse breast cancer models, we show that the chromatin remodeling subunit bromodomain PHD finger transcription factor (BPTF) suppresses natural killer (NK) cell antitumor activity in tumor microenvironment (TME). In culture, BPTF direct cytotoxicity receptor (NCR) mediated NK cytolytic to and human lines, demonstrating conserved functions. Blocking NCR1 vivo rescues KD weights, its importance for control of growth. We discovered occupies heparanase (Hpse)...