A5004529726- Immune responses and vaccinations
- Cancer Genomics and Diagnostics
- Gut microbiota and health
- Pancreatic and Hepatic Oncology Research
- Cancer Immunotherapy and Biomarkers
- Sepsis Diagnosis and Treatment
- Thermal Regulation in Medicine
- Genetic Syndromes and Imprinting
- Single-cell and spatial transcriptomics
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- COVID-19 Clinical Research Studies
- Clinical Laboratory Practices and Quality Control
- Epigenetics and DNA Methylation
- Body Composition Measurement Techniques
- Inflammatory Bowel Disease
- Immune cells in cancer
Fraunhofer Institute for Interfacial Engineering and Biotechnology
2023-2024
Vienna Biocenter
2023-2024
University of Vienna
2024
Medical University of Vienna
2023-2024
Max Perutz Labs
2023-2024
Inflammatory bowel diseases (IBDs) are a global health issue with an increasing incidence. Although the pathogenesis of IBDs has been investigated intensively, etiology remains enigmatic. Here, we report that interleukin-3 (Il-3)-deficient mice more susceptible and exhibit increased intestinal inflammation during early stage experimental colitis. IL-3 is locally expressed in colon by cells harboring mesenchymal stem cell phenotype protects promoting recruitment splenic neutrophils high...
Background Liquid biopsy based on cell-free DNA (cfDNA) is an established approach in clinical diagnostics. In recent years, a fraction of cfDNA comprising short fragments has been discovered, that enriched at gene promoters and binding sites DNA-binding proteins. However, the diagnostic potential such double-stranded (footprint DNA) remains to be fully explored. Therefore, we characterized utility footprint septic patients. Methods We for size selection subsequent high-throughput sequencing...
In order to identify biomarkers for earlier prediction of COVID-19 outcome, we collected blood samples from patients with fatal outcomes (non-survivors) and positive clinical (survivors) at ICU admission after seven days. survivors non-survivors showed significantly different transcript levels 93 genes in whole already as revealed by RNA-Seq. These differences became even more pronounced day 7, resulting 290 differentially expressed genes. Many identified play a role the differentiation...
Abstract Segmented filamentous bacteria (SFB) are members of the commensal intestinal microbiome. They known to contribute postnatal maturation gut immune system, but also augment inflammatory conditions in chronic diseases such as Crohn's disease. Living primary tissue slices ultrathin multicellular sections intestine and provide a unique opportunity analyze tissue‐specific responses ex vivo . This study aimed investigate whether supplementation flora with SFB promotes T helper 17 (Th17)...
Here, we present a method for enrichment of double-stranded cfDNA with an average length ∼40 bp from high-throughput DNA sequencing. This class is enriched at gene promoters and binding sites transcription factors or structural DNA-binding proteins, so that genome-wide footprint directly captured liquid biopsies. In short healthy individuals, find significant 203 factor motifs. Additionally, signals specific genomic regions correlate negatively methylation, positively H3K4me3 histone...
Abstract The diagnostic potential of short double-stranded cell-free DNA (cfDNA) in blood plasma has not been recognized, yet. Here, we present a method for enrichment cfDNA with an average length about 40 base pairs from high-throughput sequencing. This class is enriched at gene promoters and binding sites transcription factors or structural DNA-binding proteins, so that genome-wide footprint directly captured liquid biopsies. In healthy individuals, found significant 203 factor motifs....