A5026515384- Physiological and biochemical adaptations
- Identification and Quantification in Food
- Genetic diversity and population structure
- Meat and Animal Product Quality
- MicroRNA in disease regulation
- Aquaculture Nutrition and Growth
- Marine Biology and Ecology Research
- Ichthyology and Marine Biology
- Fish Ecology and Management Studies
- High Altitude and Hypoxia
- Aquaculture disease management and microbiota
- Cephalopods and Marine Biology
- Genomics and Phylogenetic Studies
- Metabolomics and Mass Spectrometry Studies
- Iron Metabolism and Disorders
- Invertebrate Immune Response Mechanisms
- Molecular Biology Techniques and Applications
- Aquatic Invertebrate Ecology and Behavior
- Heat shock proteins research
- Erythropoietin and Anemia Treatment
- Cancer, Hypoxia, and Metabolism
- Zebrafish Biomedical Research Applications
- Marine Bivalve and Aquaculture Studies
- Epigenetics and DNA Methylation
- Genetic and phenotypic traits in livestock
Shanghai Ocean University
2016-2025
Collaborative Innovation Centre for Advanced Ship and Deep-Sea Exploration
2015-2022
First People’s Hospital of Jingmen
2021
Ministry of Education of the People's Republic of China
2004-2016
Nantong University
2016
Second Xiangya Hospital of Central South University
2015
Central South University
2015
Ministry of Agriculture and Rural Affairs
2014
Wuhan University
2012
Zhongnan Hospital of Wuhan University
2012
The antifreeze glycoprotein-fortified Antarctic notothenioid fishes comprise the predominant fish suborder in isolated frigid Southern Ocean. Their ecological success undoubtedly entailed evolutionary acquisition of a full suite cold-stable functions besides protection. Prior studies adaptive changes these teleost generally examined single genotype or phenotype. We report here genome-wide investigations transcriptional and genomic associated with cold adaptation. sequenced characterized...
Simultaneous multiclass classification of tumor types is essential for future clinical implementations microarray-based cancer diagnosis. In this study, we have combined genetic algorithms (GAs) and all paired support vector machines (SVMs) identification. The predictive features been selected through iterative SVMs/GAs, recursive feature elimination post-processing steps, leading to a very compact cancer-related gene set. Leave-one-out cross-validations yielded accuracies 87.93% the...
Abstract Transcriptional plasticity is a major driver of phenotypic differences between species. The lower temperature limit (LTL), namely the end survival temperature, an important trait delimiting geographical distribution species, however, genetic mechanisms are poorly understood. We investigated inter-species transcriptional diversification in cold responses zebrafish Danio rerio and tilapia Oreochromis niloticus , which were reared at common (28 °C) but have distinct LTLs. identified...
Abstract Background The Southern Ocean is the coldest ocean on Earth but a hot spot of evolution. bottom-dwelling Eocene ancestor Antarctic notothenioid fishes survived polar marine glaciation and underwent adaptive radiation, forming >120 species that fill all water column niches today. Genome-wide changes enabling physiological adaptations rapid expansion notothenioids remain poorly understood. Results We sequenced compared 2 genomes—the cold-adapted neutrally buoyant toothfish...
Hepcidin is a small bioactive peptide with dual roles as an antimicrobial and the principal hormonal regulator of iron homeostasis in human mouse. homologs very similar structures are found lower vertebrates, all comprise approximately 20-25 amino acids 8 highly conserved cysteines forming 4 intramolecular disulfide bonds, giving hepcidin hairpin structure. Hepcidins particularly diverse teleost fishes, which may be related to diversity aquatic environments varying degree pathogen challenge,...
Heat shock protein 60 (HSP60) is a highly conserved and multi-functional molecular chaperone that plays an essential role in both cellular metabolism stress response. Portunus trituberculatus important marine fishery aquaculture species, water salinity condition influenced its artificial propagations significantly. In order to investigate the function of P. HSP60 against osmotic stress, gene was firstly cloned. The full-length cDNA PtHSP60 contains 1,743 nucleotides encoding 577 amino acids...
Abstract The mechanisms by which the eggs of Antarctic notothenioid fishes avoid freezing are not fully understood. Zona pellucida proteins (ZPs) constituents chorion forms a protective matrix surrounding egg. Here we report occurrence temperature-related gene expansion and acquisition unusual ice melting-promoting (IMP) activity in family ZPs (AnnotoZPs). Members AnnotoZPs shown to bind with non-colligatively depress melting point solution range 0.26 0.65 °C at moderate concentration. Eggs...
Background: Erythropoietin (EPO) is a glycoprotein hormone that plays principal regulatory role in erythropoiesis and initiates cell homeostatic responses to environmental challenges.The Qinghai-Tibet Plateau natural laboratory for hypoxia adaptation.Gymnocypris dobula highly specialized plateau schizothoracine fish restricted > 4500 m high-altitude freshwater rivers ponds the Plateau.The of EPO adaptation unknown.Results: The receptor genes from G. four other various altitudinal habitats...
Understanding the primary molecular events driving temperature-dependent sex reversal (TSR) has proven challenging, particularly in distinguishing these from secondary effects of sexual differentiation. The mechanisms translating temperature into a sex-determining signal fish are still largely unknown. Through combined transcriptomic and genome-wide histone methylation analyses gonads Nile tilapia (Oreochromis niloticus) exposed to normal elevated temperatures, we observed significant...
Hepcidin is a key regulator of systemic iron homeostasis, which essential for maintaining balance and cellular health. To investigate its role in zebrafish, we empolyed hepcidin knockout model. Morphological histological analyses revealed pale livers significant accumulation hep−/− particularly liver, skin, egg tissues. RNA sequencing identified 1,424 differentially expressed genes (DEGs) between wild-type (WT) with enrichment pathways related to ferroptosis, fatty acid degradation, heme...
Abstract The Antarctic icefish, a family (Channichthyidae) of teleosts within the perciform suborder Notothenioidei, are only known vertebrates without oxygen‐transporting haemoglobins and that largely devoid circulating erythrocytes. To elucidate evo‐devo mechanisms underpinning suppressed erythropoiesis in we conducted comparative studies on transcriptomes micro RNA omes primary haematopoietic tissues between an icefish ( Chionodraco hamatus ) two red‐blooded notothenioids Trematomus...
Hadal trenches are the deepest known areas of ocean. Amphipods considered to be dominant scavengers in hadal food webs. The studies on structure and function intestinal microbiotas largely lacking. Here, three amphipods, Hirondellea gigas , Scopelocheirus schellenbergi Alicella gigantea from Mariana Trench, Marceau New Britain respectively, were investigated. taxonomic analysis identified 358 microbial genera commonly shared within amphipods. Different amphipod species possessed their own...
Hepcidin is a small peptide composed of signal peptide, propeptide, and the bioactive mature from N terminal to C terminal. Mature hepcidin an antibacterial iron regulator with eight highly conserved cysteines forming four intramolecular disulfide bonds, giving it β sheet hairpin-like structure. homologs are found in variety vertebrates, especially fish, their diversity may be associated different habitats levels pathogens. Dissostichus mawsoni, Antarctic notothenioid fish that lives coldest...
Nile tilapia (Oreochromis niloticus) is one of the most important food fishes in global aquaculture. The optimal rearing temperature for 27–30 °C; however, some Asian breeding areas, such as south China, water temperatures summer frequently exceed 35 °C several days. Potential effects long-term exposure to high on survival and metabolism are unclear. In this study, genetically improved farmed tilapia, age six weeks, were exposed 28, 32, 36 15 weeks. Mean rates tolerance hypoxia significantly...
An in vitro immunization (IVI) protocol enables antigen specific antibody production from L-Leucyl-L-Leucine methyl ester (LLME)-treated human peripheral blood lymphocytes (PBL) upon stimulation the presence of IL-2, IL-4, and muramyl dipeptide. In course our studies, we have found that IL-10 added at sensitization significantly augmented level LLME-treated PBL. present study, tried to demonstrate role augmentation an IVI by clarifying cytokine expression profiles CD4+ CD8+ T cells. The...
Many species of Schizothoracine, a subfamily Cyprinidae, are highly endemic to the Qinghai-Tibetan Plateau (QTP). To characterize adaptive changes associated with Schizothoracine expansion at high altitudes, we sequenced tissue transcriptomes two highland and subhighland Schizothoracines analyzed gene evolution patterns by comparing lowland cyprinids. Phylogenetic tree reconstruction divergence time estimation indicated that common ancestor fish lived ∼32.7 million years ago (MYA),...