A5100321758- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Cellular transport and secretion
- ATP Synthase and ATPases Research
- Endoplasmic Reticulum Stress and Disease
- RNA regulation and disease
- Venomous Animal Envenomation and Studies
- Biochemical and Molecular Research
- RNA and protein synthesis mechanisms
- Marine Invertebrate Physiology and Ecology
- Adenosine and Purinergic Signaling
- Enzyme Structure and Function
- Lipid Membrane Structure and Behavior
- Nanoparticle-Based Drug Delivery
- Calcium signaling and nucleotide metabolism
- Ion channel regulation and function
- Smart Grid Energy Management
- Nuclear Structure and Function
- Blood disorders and treatments
- Genomic variations and chromosomal abnormalities
- Water-Energy-Food Nexus Studies
- Cell death mechanisms and regulation
- Natural product bioactivities and synthesis
- Service and Product Innovation
- Sustainable Supply Chain Management
Huazhong Agricultural University
2021-2025
University of Utah
2013-2024
China Pharmaceutical University
2018-2021
University of Hong Kong
2021
First Affiliated Hospital of Soochow University
2019
Soochow University
2019
Monash University
2017
University of California, Berkeley
2017
Politecnico di Milano
2017
Australian Regenerative Medicine Institute
2017
Despite being engineered to avoid renal clearance, many cationic polymer (polycation)-based siRNA nanoparticles that are used for systemic delivery rapidly eliminated from the circulation. Here, we show a component of filtration barrier—the glomerular basement membrane (GBM)—can disassemble cyclodextrin-containing (CDP)-based and, thereby, facilitate their rapid elimination Using confocal and electron microscopies, positron emission tomography, compartment modeling, demonstrate...
The cellular machine Cdc48 functions in multiple biological pathways by segregating its protein substrates from a variety of stable environments such as organelles or multi-subunit complexes. Despite extensive studies, the mechanism has remained obscure, and reported structures are inconsistent with models substrate translocation proposed for other AAA+ ATPases (adenosine triphosphatases). Here, we report 3.7-angstrom-resolution structure complex an adaptor native substrate. engages adopting...
Many important cellular membrane fission reactions are driven by ESCRT pathways, which culminate in disassembly of ESCRT-III polymers the AAA ATPase Vps4. We report a 4.3 Å resolution cryo-EM structure active Vps4 hexamer with its cofactor Vta1, ADP·BeFx, and an substrate peptide. Four subunits form helix whose interfaces consistent ATP binding, is stabilized binds The fifth subunit approximately continues this but appears to be dissociating. final completes notched-washer configuration as...
The hexameric AAA ATPase Vps4 drives membrane fission by remodeling and disassembling ESCRT-III filaments. Building upon our earlier 4.3 Å resolution cryo-EM structure (<xref ref-type="bibr" rid="bib29">Monroe et al., 2017</xref>), we now report a 3.2 of bound to an peptide substrate. new reveals that the approximates β-strand conformation whose helical symmetry matches five subunits it contacts directly. Adjacent make equivalent interactions with successive substrate dipeptides...
Many members of the AAA+ ATPase family function as hexamers that unfold their protein substrates. These AAA unfoldases include spastin, which plays a critical role in architecture eukaryotic cells by driving remodeling and severing microtubules, are cytoskeletal polymers tubulin subunits. Here, we demonstrate human spastin binds weakly to unmodified peptides from C-terminal segment α1A/B. A peptide comprising alternating glutamate tyrosine residues more tightly, is consistent with known...
Many AAA+ ATPases form hexamers that unfold protein substrates by translocating them through their central pore. Multiple structures have shown how a helical assembly of subunits binds single strand substrate, and indicate translocation results from the ATP-driven movement one end to other end. To understand more complex are bound translocated, we demonstrated linear cyclic versions peptides bind S. cerevisiae ATPase Vps4 with similar affinities, determined cryo-EM peptide complexes. The in...
Abstract The p97 AAA+ATPase is an essential and abundant regulator of protein homeostasis that plays a central role in unfolding ubiquitylated substrates. Here we report two cryo-EM structures human complex with its p47 adaptor. One the conformations six-fold symmetric, corresponds to previously reported p97, lacks bound substrate. other structure adopts helical conformation, displays substrate running extended conformation through pore hexamer, resembles for AAA unfoldases. These findings...
DNA manipulation technology is facing more challenges in the postgenomics era. More and nanomaterials have been investigated for their potential implications developing better gene technology. In this study, we reported beneficial effect of single-walled carbon nanotubes (SWCNTs) multi-walled (MWCNTs) enhancing specificity total efficiency long (14 kb) PCR. Hydroxylic carboxylic (CNTs) had similar effects. Nanotubes could become another component improvements amplification DNA.
In recent years, increasing numbers of luxury groups have adopted sustainable practices in their supply chains (sourcing, manufacturing, logistics, distribution, servicing, waste and recycling). However, the report from Greenpeace International organization (2014) indicates that some brands/companies did not actively conduct to produce items, which is likely attributed cost risks caused by such outweighing benefits. This, extent, due failure developing collaborative practices. Specifically,...
The development of enzymatic biofuel cells has been plagued by the high cost enzyme purification and low efficiency fuel oxidation. Here, we demonstrate a protein purification-free approach to assemble an alcohol dehydrogenase aldehyde cascade-based bioanode for use in methanol cell. Each was fused different sequence-specific zinc finger DNA-binding protein. domains serve as both tags isolate enzymes from crude cell lysates well anchors immobilize on DNA-modified multiwalled carbon...
Abstract Citric acid accumulation is an essential process in citrus fruits that determines fruit flavor and marketability. The MBW complex transcription factor genes, CsAN11, CsAN1, CsPH4 play key roles regulating citric accumulation. Although how to regulate CsAN1 or was widely investigated, studies on the regulation of CsAN11 are scarce. In this study, we characterized AP2/ERF (APETALA2/ethylene response factor) gene CsAIL6, which lowly expressed high-acid varieties highly low-acid...
The multiplex ligation-dependent probe amplification (MLPA) method was used to analyze 118 DNA samples from 90 alpha-thalassemia (alpha-thal) patients and 28 normal persons Southern China, where the main causes of alpha-thal are three large deletions (-alpha3.7, -alpha4.2, --SEA) two point mutations in alpha-globin gene cluster on chromosome 16. results, detected by P140B HBA kit, were complete concordance with results polmymerase chain reaction (m-PCR) real-time PCR. advantages limitations...
Context: Novel antimalarial agents are in demand due to the emergence of multidrug resistant strains. Ginseng, a medicinal plant with antiparasitic activity, contains components that can be used treat tropical disease malaria.
With increasing levels of drought across the world together with rising demand freshwater and energy consumption, interdependence between power water sectors is becoming increasingly important. However, as system operators have traditionally operated their systems in isolation, there a lack understanding interactions these systems. This paper bridges this information gap by developing 2D 3D visualization prototypes for interdependent distribution These aim to display monitor variety...
Fish venoms are often poorly studied, in part due to the difficulty obtaining, extracting, and storing them. In this study, we characterize cardiovascular neurotoxic effects of from following six species fish: cartilaginous stingrays Neotrygon kuhlii Himantura toshi, bony fish Platycephalus fucus, Girella tricuspidata, Mugil cephalus, Dentex tumifrons. All (10-100 μg/kg, i.v.), except G. tricuspidata P. fuscus, induced a biphasic response on mean arterial pressure (MAP) anesthetised rat....
The LiP infinite double-helix chain gives us the unique opportunity to investigate structure at atomic level. Based on density-functional theory, we characteristic vibrational modes of this peculiar structure. Differently from single-wall carbon nanotubes, a dipole mode in rise high infrared intensity. Owing LiP, frequency out-of-phase radical breathing is lower than that in-phase one. It similar condition DNA. Furthermore, instead one, has highest Raman Most importantly, our finding...
The ESCRT pathway's AAA+ ATPase, Vps4p, remodels ESCRT-III complexes to drive membrane fission. Here, we use peptide binding assays further the understanding of substrate specificity and mechanism autoinhibition. Our results reveal unexpected sequence preference groove an elegant regulation that couples localization with release from