A5088133628- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Geochemistry and Geologic Mapping
- Geological and Geochemical Analysis
- earthquake and tectonic studies
- Ubiquitin and proteasome pathways
- Corrosion Behavior and Inhibition
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Salmonella and Campylobacter epidemiology
- Drug Transport and Resistance Mechanisms
- Dementia and Cognitive Impairment Research
- Monoclonal and Polyclonal Antibodies Research
- Aldose Reductase and Taurine
- Biopolymer Synthesis and Applications
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Paleontology and Stratigraphy of Fossils
- Cholesterol and Lipid Metabolism
- Hormonal and reproductive studies
- Receptor Mechanisms and Signaling
- DNA Repair Mechanisms
University of Ottawa
2016-2025
Joint Research Center on Systems and Personalized Pharmacology
2022
Bipar
2016
Faculty (United Kingdom)
2016
University of Southern California
2014
McGill University
2011
University of Michigan
2010
Ottawa Institute of Systems Biology
2007
Université Laval
2003-2004
Wilfrid Laurier University
2003
Abstract Mutations in proteins like FUS which cause Amyotrophic Lateral Sclerosis (ALS) result the aberrant formation of stress granules while ALS-linked mutations other impede elimination granules. Repeat expansions C9ORF72, major ALS, reduce C9ORF72 levels but how this impacts is uncertain. Here, we demonstrate that associates with autophagy receptor p62 and controls by autophagy. This requires to associate via Tudor protein SMN proteins, including FUS, are symmetrically methylated on...
WDR5 (WD40 repeat protein 5) is an essential component of the human trithorax-like family SET1 [Su(var)3-9 enhancer-of-zeste trithorax 1] methyltransferase complexes that carry out trimethylation histone 3 Lys4 (H3K4me3), play key roles in development and are abnormally expressed many cancers. In present study, we show interaction between peptides from catalytic domain MLL (mixed-lineage leukaemia protein) (KMT2) can be antagonized with a small molecule. Structural biophysical analysis this...
The tail of replication-dependent histone H3.1 varies from that replication-independent H3.3 at the amino acid located position 31 in plants and animals, but no function has been assigned to this residue demonstrate a unique conserved role for during replication. We found TONSOKU (TSK/TONSL), which rescues broken replication forks, specifically interacts with via recognition alanine by its tetratricopeptide repeat domain. Our results indicate genomic instability absence ATXR5/ATXR6-catalyzed...
The SMYD (SET and MYND domain) family of lysine methyltransferases (KMTs) plays pivotal roles in various cellular processes, including gene expression regulation DNA damage response. Initially identified as genuine histone methyltransferases, specific members this have recently been shown to methylate non-histone proteins such p53, VEGFR, the retinoblastoma tumor suppressor (pRb). To gain further functional insights into KMTs, we generated protein interaction network for three different...
APOBEC3G (A3G) is a host-encoded protein that potently restricts the infectivity of broad range retroviruses. This can occur by mechanisms dependent on catalytic activity, resulting in mutagenic deamination nascent viral cDNA, and/or other means are independent its activity. It not yet known to what extent deamination-independent processes contribute overall restriction, how they exactly work or regulated. Here, we show alanine substitution either tryptophan 94 (W94A) 127 (W127A)...
Conditional gene expression systems that enable inducible and reversible transcriptional control are essential research tools have broad applications in biomedicine biotechnology. The reverse tetracycline activator is a canonical system for engineered enables graded gratuitous modulation of target transcription eukaryotes from yeast to human cell lines transgenic animals. However, the has tendency activate even absence this leaky impedes its use. Here, we identify single amino-acid...
Lysine acetylation (Kac), an abundant post-translational modification (PTM) in prokaryotes, regulates various microbial metabolic pathways. However, no studies have examined protein Kac at the microbiome level, and it remains unknown whether level is altered patient microbiomes. Herein, we use a peptide immuno-affinity enrichment strategy coupled with mass spectrometry to characterize microbiome, which successfully identifies 35,200 peptides from or human proteins gut samples. We demonstrate...
We investigated two critical aspects of rose Bengal (RB) photosensitized protein cross-linking that may underlie recently developed medical applications. Our studies focused on the binding RB to collagen by physical interaction and effect this certain amino acids photochemistry. Molecular dynamics simulations free-energy calculation techniques, complemented with isothermal titration calorimetry, provided insight into between a collagen-like peptide (CLP) at atomic level. Electrostatic...
The methyltransferase activity of the trithorax group (TrxG) protein MLL1 found within its COMPASS (complex associated with SET1)-like complex is allosterically regulated by a four-subunit composed WDR5, RbBP5, Ash2L, and DPY30 (also referred to as WRAD). We report structural evidence showing that in WRAD, concave surface Ash2L SPIa ryanodine receptor (SPRY) domain binds cluster acidic residues, D/E box, RbBP5. Mutational analysis shows residues forming Ash2L/RbBP5 interface are important...
Antiandrogens are commonly used to treat androgen-dependent disorders. The currently drugs unfortunately possess very weak affinity for the human AR (hAR), thus indicating need develop new high-affinity steroidal antiandrogens. Our compounds specially designed impede repositioning of mobile carboxyl-terminal helix 12, which blocks ligand-dependent transactivation function (AF-2) located in ligand-binding domain (ARLBD). Using crystal structures hARLBD, we first found that H12 could be...
Protein-protein interactions (PPIs) form the foundation of any cell signaling network. Considering that PPIs are highly dynamic processes, cellular assays often essential for their study because they closely mimic biological complexities environments. However, incongruity may be observed across different PPI when investigating a protein partner interest; these discrepancies can partially attributed to fusion large functional moieties, such as fluorescent proteins or enzymes, which yield...
We reported a negative-selection strategy for protein methylation profiling by suppressing the charge of unmethylated peptides <italic>via</italic> two chemical reactions.
Gold-bearing quartz vein deposits in the Val-d'Or area of southern Abitibi greenstone belt are classified two categories based on structural and chronologic relationships. Young ( 2690 Ma) feature commonly deformed orebodies that cut by dikes various composition. Such occur at Siscoe, Kiena, Orion, Norlartic mines. The Main orebody is crosscut a tonalite dike which has been dated U-Pb 2692 + or - 2 Ma, agreement with data. These observations show gold mineralization occurs least different...
In the budding yeast Saccharomyces cerevisiae, lysine acetyltransferase NuA4 has been linked to a host of cellular processes through acetylation histone and non-histone targets. To discover proteins regulated by NuA4-dependent acetylation, we performed genome-wide synthetic dosage lethal screens identify genes whose overexpression is toxic non-essential deletion mutants. The resulting genetic network identified novel link between septin proteins, group highly conserved GTP-binding that...
Journal Article Military Parents' Perceptions of Public School Supports for Their Children Get access Ruth Berkowitz, Berkowitz Berkowitz,MA, is a doctoral candidate, Social Work, University Southern California, Los Angeles. Kris M. T. De Pedro, PhD, assistant professor, College Educational Studies, Chapman University, Orange, CA. Julie Couture,MSW, student, Rami Benbenishty,PhD, Louis and Gabi Weisfeld Bar Ilan Israel. Ron Avi Astor, Richard Ann L. Thor Professor Urban Development, Work...
Campylobacter jejuni is a prevalent cause of bacterial gastroenteritis in humans worldwide. The mechanisms by which C. survives stomach acidity remain undefined. In the present study, we demonstrated that ferric uptake regulator (Fur) plays an important role acid survival and acid-induced cross-protection against oxidative stress. A Δfur mutant was more sensitive to than wild-type strain. Profiling stimulon allowed us uncover Fur-regulated genes under acidic conditions. particular, Fur found...
In the Val d'Or camp, Archean Abitibi greenstone belt, Canada, numerous gold-mineralized second- and third-order fault zones are spatially associated with transcrustal Cadillac Tectonic Zone (CTZ). This situation is used to test whether fluid systems in CTZ have a similar structural timing those gold-hosting structures, hence could represent main conduit camp. The at Orenada No. 2 contains structurally complex vein systems, mineralized quartz-tourmaline veins related both D oblique-reverse...
Red fluorescent proteins (RFPs) are used extensively in chemical biology research as fluorophores for live cell imaging, partners FRET pairs, and signal transducers biosensors. For all of these applications, brighter RFP variants desired. Here, we rational design to increase the quantum yield monomeric RFPs order improve their brightness. We postulated that could by restricting conformational degrees freedom chromophore. To test our hypothesis, introduced aromatic residues above chromophore...