A5063332605- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Nitric Oxide and Endothelin Effects
- Receptor Mechanisms and Signaling
- Telomeres, Telomerase, and Senescence
- Genetics, Aging, and Longevity in Model Organisms
- Neuroscience and Neuropharmacology Research
- Dermatology and Skin Diseases
- Muscle Physiology and Disorders
- interferon and immune responses
- Cardiac Ischemia and Reperfusion
- Mitochondrial Function and Pathology
- Mesenchymal stem cell research
- Asthma and respiratory diseases
- Adipose Tissue and Metabolism
- IL-33, ST2, and ILC Pathways
- Lung Cancer Treatments and Mutations
- Birth, Development, and Health
- Immune Cell Function and Interaction
- Laser Applications in Dentistry and Medicine
- Ecology and Conservation Studies
- Animal Disease Management and Epidemiology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cytokine Signaling Pathways and Interactions
- Autophagy in Disease and Therapy
Chung-Ang University
2012-2025
Korea University
2017
Inje University
2006-2007
Pusan National University
2007
Seoul National University
2005-2006
Hokkaido University
2002-2005
Yeungnam University
2005
Hiroshima University
2002
Higashihiroshima Medical Center
2002
The nucleotide sequence of chicken Mx cDNA was reported earlier using the White Leghorn breed in Germany, but it showed no enhanced resistance to viruses. In this study, sequences were determined many breeds. A total 25 substitutions, which 14 deduced cause amino acid exchanges, detected, suggesting that gene is very polymorphic. Transfected cell clones expressing mRNA established after constructed with an expression vector and introduced into mouse 3T3 cells, genes from some breeds...
Cilostazol is a specific inhibitor of 3'-5'-cyclic adenosine monophosphate (cAMP) phosphodiesterase, which widely used to treat ischemic symptoms peripheral vascular disease. Although cilostazol has been shown exhibit vasodilator properties as well antiplatelet and anti-inflammatory effects, its cellular mechanism in microglia unknown. In the present study, we assessed effect on production pro-inflammatory mediators lipopolysaccharide (LPS)-stimulated murine BV2 microglia.We examined effects...
Purpose Both telomere length and mitochondrial function are accepted as reflective indices of aging. Recent studies have shown that dysfunction may influence impaired biogenesis function. However, there has been no study regarding the possible association between in humans. Therefore, purpose was to identify any relationships Methods The present included 129 community-dwelling, elderly women. leukocyte DNA copy number were measured using a quantitative real-time polymerase chain reaction...
Objective It has been reported that women benefit from the maintenance of telomere length by estrogen. Exercise may favorably influence length, although results are inconsistent regarding duration and type exercise cell used to measure length. The purpose this study was investigate relationship between habitual physical in peripheral blood mononuclear cells (PBMCs) postmenopausal women. Postmenopausal were chosen as participants because they typically estrogen deficient. Methods This...
We compared mitochondrial function, morphology, and proteome in the rat normal gastric cell line RGM-1 human cancer AGS. Total numbers cross-sectional sizes of mitochondria were smaller AGS cells. Mitochondria cells deformed consumed less oxygen. Confocal microscopy indicated that inner membrane potential was hyperpolarized Ca(2+) concentration elevated Interestingly, two-dimensional electrophoresis proteomics on mitochondria-enriched fraction revealed high expression four proteins cells:...
Human adipose tissue-derived mesenchymal stem cells (hASCs) have the power to differentiate into various cell types including chondrocytes, osteocytes, adipocytes, neurons, cardiomyocytes, and smooth muscle cells. We characterized functional expression of ion channels after transforming growth factor-β 1 (TGF-β )-induced differentiation hASCs, providing insights vascular The treatment hASCs with TGF-β dramatically increased contraction a collagen-gel lattice levels specific genes for...
Abstract Gestational vitamin D insufficiency is related with increased risks of various diseases and poor health outcomes later in life. Telomere length at birth or early life known to be a predictor individual health. Both telomere are conditions, concentrations associated leukocyte lengths women. We investigated the association between maternal newborn lengths. This cross‐sectional study included 106 healthy pregnant women without adverse obstetric their offspring. examined age, weight...
We examined the effects of acute hypoxia on Ba2+-sensitive inward rectifier K+ (K(IR)) current in rabbit coronary arterial smooth muscle cells. The amplitudes K(IR) was definitely higher cells from small-diameter (<100 microm) (SCASMC, -12.8 +/- 1.3 pA/pF at -140 mV) than those large-diameter (>200 microm, LCASMC, -1.5 0.1 pA pF(-1)). Western blot analysis confirmed that Kir2.1 protein expressed SCASMC but not LCASMC. Hypoxia activated much more KIR currents symmetrical 140 K+. This effect...
Influenza A virus (FLUAV) causes serious diseases in both poultry and humans. Various host proteins, including Mx1, are considered candidates for avian influenza (AI) resistance. After infecting Jeju Native chicken embryo fibroblasts (CEFs) with three types of AI viruses, we performed gene expression profiling, identified single nucleotide polymorphisms (SNPs) through RNA-sequencing, confirmed phenotypes showing antiviral activity vitro. Highly pathogenic viruses upregulated FGF2, LYN, FLT4...
Maternal stress during pregnancy can profoundly affect offspring health, increasing the risk of psychiatric disorders, metabolic diseases, and gastrointestinal problems. In this study, effects high prenatal corticosterone exposure on gene expression in brain small intestine rat were investigated via RNA-sequencing analysis. Pregnant rats divided into two groups: Corti.Moms injected with daily, while Nor.Moms given saline injections. Their labeled as Corti.Pups Nor.Pups, respectively. The...
Objective— We investigated the effects of angiotensin II (Ang II) on inward rectifier K + (Kir) channels in small-diameter coronary arterial smooth muscle cells (SCASMCs) control and isoproterenol (Iso)-induced hypertrophied rabbits. Methods Results— Kir current amplitude channel protein expression were definitely lower Iso-induced model than control. In a pressurized experiment, 15 mmol/L -induced vasodilation was greater arteries model. Ang reduced concentration-dependent manner, this...
We examined the effects of protein kinase C (PKC) inhibitor staurosporine (ST) on voltage-dependent K+ (KV) channels in rabbit coronary arterial smooth muscle cells. ST inhibited KV current a dose-dependent manner with Kd value 1.3 μM. The inhibition by was between −30 and +10 mV. additive throughout activation voltage range. rate constants association dissociation were 0.63 μM−1 s−1 0.92 s−1, respectively. produced use-dependent current. shifted curve to more positive potentials but did not...
We investigated the effect of phenylalkylamine Ca2+ channel inhibitor verapamil on voltage-dependent K+ (Kv) channels in rabbit coronary arterial smooth muscle cells using a whole-cell patch clamp technique. Verapamil reduced Kv current amplitude concentration-depenent manner. The apparent Kd value for inhibition was 0.82 μM. Although had no activation kinetics, it accelerated decay rate inactivation. constants association and dissociation by were 2.20±0.02 μM−1 s−1, 1.79±0.26 respectively....
We studied inward rectifier K+ (Kir) channels in smooth muscle cells isolated from rabbit coronary arteries. In small- (<100 microm, SCASMC) and medium-diameter (100 approximately 200 MCASMC) arteries, Kir currents were clearly identified (11.2 +/- 0.6 4.2 pA pF at -140 mV SCASMC MCASMC, respectively) that inhibited by Ba(2+) (50 microm). By contrast, a very low current density (1.6 0.4 pF) was detected large-diameter arteries (>200 LCASMC). The presence of Kir2.1 protein confirmed Western...
Despite increased evidence of bio-activity following far-infrared (FIR) radiation, susceptibility cell signaling to FIR radiation-induced homeostasis is poorly understood. To observe the effects FIR-radiated materials-coated fabric was put on experimental rats or applied L6 cells, and microarray analysis, quantitative real-time polymerase chain reaction, wound healing assays were performed. Microarray analysis revealed that messenger RNA expressions rat muscle stimulated by radiation in a...
The effects of Alnus sibirica (AS) extracts on cytokine expression induced by inflammatory stimulants were examined in human dermal fibroblasts (HDFs) and RAW264.7 cells. anti-oxidative effect cell viability AS evaluated, four with the highest selected. HDFs cells treated stimulants, cytokines involved acute (IL-6 IL-10) chronic (IL-18) inflammation, initiation immune response (IL-33), non-specific responses (IL-1β, IL-8, TNF-α) determined using a reverse-transcription polymerase chain...