A5046600646- Genetic factors in colorectal cancer
- Colorectal Cancer Treatments and Studies
- Cancer Genomics and Diagnostics
- Cancer-related Molecular Pathways
- Metabolomics and Mass Spectrometry Studies
- Cancer, Hypoxia, and Metabolism
- Colorectal Cancer Screening and Detection
- Digestive system and related health
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Epigenetics and DNA Methylation
- Genetic Associations and Epidemiology
- RNA modifications and cancer
- Cancer Research and Treatments
- Inflammatory mediators and NSAID effects
- Nutrition, Genetics, and Disease
- RNA Research and Splicing
- Neurobiology and Insect Physiology Research
- Cell Adhesion Molecules Research
- Drug Transport and Resistance Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related molecular mechanisms research
- Helicobacter pylori-related gastroenterology studies
- Cancer Immunotherapy and Biomarkers
- Genomic variations and chromosomal abnormalities
- Wnt/β-catenin signaling in development and cancer
Singapore General Hospital
2013-2023
National University of Singapore
2008-2023
National University Health System
2012-2023
Duke-NUS Medical School
2012-2023
SingHealth Duke-NUS Academic Medical Centre
2017
National Cancer Centre Singapore
2008
Institute of Molecular and Cell Biology
1994-2000
University of Arizona
1990
Current clinical strategy for staging and prognostication of colorectal cancer (CRC) relies mainly upon the TNM or Duke system. This clinicopathological stage is a crude prognostic guide because it reflects in part delay diagnosis case an advanced gives little insight into biological characteristics tumor. We hypothesized that global metabolic profiling (metabonomics/metabolomics) colon mucosae would define signatures not only discriminate malignant from normal mucosae, but also could...
Abstract Purpose: The causative genes for autosomal dominantly inherited familial adenomatous polyposis (FAP) and hereditary nonpolyposis colorectal cancer have been well characterized. There is, however, another 10% to 15% of early onset cancers (CRC) in which the genetic components are unclear. In this study, we used microarray technology systematically search differentially expressed CRC. Experimental Design: Young patients with non–FAP or non–hereditary cancer, healthy controls were age-...
Colorectal cancer is the second leading killer worldwide and presently most common among males in Singapore. The study aimed to detect changes of protein profiles associated with process colorectal tumorigenesis identify specific markers for early detection diagnosis or as potential therapeutic targets. Seven pairs tissues adjacent normal mucosa were examined by two-dimensional gel electrophoresis at basic pH range (pH 7–10). Intensity 34 spots detected statistical significance. 16...
Colorectal cancer (CRC) is a major cause of mortality in many developed countries. Effective screening strategies were called for to facilitate timely detection and promote better clinical outcome. In this study, the role fecal metabonomics non-invasive CRC was investigated. Gas chromatography/time-of-flight mass spectrometry (GC/TOFMS) utilized metabolic profiling feces obtained from 11 patients 10 healthy subjects. Concurrently, matched tumor normal mucosae surgically excised profiled....
Genome‐wide association studies (GWAS) of colorectal cancer (CRC) have been conducted primarily in European descendants. In a GWAS East Asians, we first analyzed approximately 1.7 million single‐nucleotide polymorphisms (SNPs) four with 1,773 CRC cases and 2,642 controls. We then selected 66 promising SNPs for replication genotyped them three independent 3,612 3,523 Five were further evaluated using data from additional including up to 3,290 4,339 SNP rs7229639 the SMAD7 gene was found be...
Abstract In this study, a gas chromatography/mass spectrometry (GC/MS) method was developed and validated for the metabolic profiling of human colon tissue. Each tissue sample (20 mg) ultra‐sonicated with 1 mL mixture chloroform/methanol/water in ratio 20:50:20 (v/v/v), followed by centrifugation, collection supernatant, drying, removal moisture using anhydrous toluene finally derivatization N ‐methyl‐ ‐trifluoroacetamide (MSTFA) 1% trimethylchlorosilane (TMCS). A volume µL derivatized...
The high mortality rate in colorectal cancer is mostly ascribed to metastasis, but the only clinical biomarker available for disease monitoring and prognosis carcinoembryonic antigen (CEA). However, prognostic utility of CEA remains controversial. In an effort identify novel biomarkers that could be potentially translated use, we collected secretomes from colon adenocarcinoma cell line HCT-116 its metastatic derivative, E1, using hollow fiber culture system, utilized multilectin affinity...
Hereditary mixed polyposis syndrome (HMPS) is characterized by polyps of adenomatous/hyperplastic/atypical juvenile histology that are autosomal dominantly inherited and eventually lead to colorectal cancer (CRC). Although CRC with adenomatous initiated inactivating coli (APC), the initiating event remains unclear. We aimed identify underlying germline defect in HMPS.We screened for bone morphogenesis protein receptor 1A (BMPR1A) mutation exonic sequencing, reverse-transcriptase polymerase...
Abstract Cumulative evidence shows that eicosanoids such as prostaglandins, leukotrienes, thromboxanes and hydroxy eicosatetraenoic acids play an important role in associating inflammation with human colorectal cancer (CRC). In this study ultra‐pressure liquid chromatography/tandem mass spectrometry (UPLC/MS/MS) method was developed validated for the targeted profiling of eight relevant major metabolic precursor, arachidonic acid (AA), colon. Multiple reaction monitoring (MRM) experiments...
Significance Mutations in the amino-terminal transactivation domain of tumor-suppressor p53 are mostly insertions or deletions, and result loss full-length expression. However, these changes concomitantly expression a truncated p47 isoform, which retains ability to selectively transactivate some apoptotic target genes. The selectivity appears be due default feature, stemming from lack acetylation on K382 at carboxyl terminus, requires amino terminus. Consistently, could prognosticate better...
Abstract BACKGROUND The stabilization and nuclear translocation of β‐catenin are early events in the majority sporadic colorectal carcinomas (CRC). up‐regulates c‐Myc cyclin D1, which antagonize association cyclin‐dependent kinase (Cdk) inhibitor, p27 kip1 , with Cdk2, thus allowing cell cycle progression through G1 to S‐phase. Lack is a significant predictor poor survival series 136 CRC specimens. A combination molecules same pathway may be better prognostic factor. METHODS expression...
Abstract Familial adenomatous polyposis (FAP) is an autosomal dominantly inherited form of colorectal cancer (CRC) caused by mutation in the coli ( APC ) gene. However, mutations are not detected 10–50% FAP patients. We searched for a new gene performing genome‐wide genotyping on members mutation‐negative variant family and ethnicity‐matched healthy controls. No common copy number change was found all affected using unaffected controls as baseline. A 111 kb variable (CNV) region at 3q26.1...
Background Genome-wide association studies (GWAS) in Caucasians have identified fourteen index single nucleotide polymorphisms (iSNPs) that influence colorectal cancer (CRC) risk. Methods We investigated the role of eleven iSNPs or surrogate SNPs (sSNPs), high linkage disequilibrium (LD, r2≥0.8) and within 100 kb vicinity iSNPs, 2,000 age- gender-matched Singapore Chinese (SCH) cases controls. Results Only iSNP rs6983267 at 8q24.21 sSNPs rs6695584, rs11986063, rs3087967, rs2059254, rs7226855...