A5030054669- Influenza Virus Research Studies
- Respiratory viral infections research
- Toxoplasma gondii Research Studies
- Herpesvirus Infections and Treatments
- Legionella and Acanthamoeba research
- Malaria Research and Control
- Immunotherapy and Immune Responses
- Advancements in Transdermal Drug Delivery
- Mosquito-borne diseases and control
- Cytomegalovirus and herpesvirus research
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Dermatology and Skin Diseases
- Parasites and Host Interactions
- Heme Oxygenase-1 and Carbon Monoxide
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Viral Infections and Vectors
- vaccines and immunoinformatics approaches
- Viral gastroenteritis research and epidemiology
- HIV Research and Treatment
- Parasitic Diseases Research and Treatment
- Parasite Biology and Host Interactions
- Animal Disease Management and Epidemiology
- Hepatitis B Virus Studies
- Parasitic Infections and Diagnostics
Jilin University
2011-2025
Kyung Hee University
2016-2025
Kyung Hee University Medical Center
2014-2024
Northwest A&F University
2024
Affiliated Hospital of Southwest Medical University
2018-2021
Emory University
2005-2019
Georgia State University
2018-2019
Yanbian University
2014
Korea National University of Transportation
2011-2012
US Biologic (United States)
2011-2012
ABSTRACT Recurrent outbreaks of highly pathogenic avian influenza virus pose the threat pandemic spread lethal disease and make it a priority to develop safe effective vaccines. Influenza virus-like particles (VLPs) have been suggested be promising vaccine approach. However, VLP-induced immune responses, their roles in inducing memory responses cross-protective immunity not investigated. In this study, we developed VLPs containing A/PR8/34 (H1N1) hemagglutinin (HA) matrix (M1) proteins...
Background Current influenza vaccines based on the hemagglutinin protein are strain specific and do not provide good protection against drifted viruses or emergence of new pandemic strains. An vaccine that can confer cross-protection antigenically different A strains is highly desirable for improving public health. Methodology/Principal Findings To develop a cross protective vaccine, we generated virus-like particles containing conserved M2 in membrane-anchored form (M2 VLPs), investigated...
Influenza virus-like particles (VLPs) are a promising cell culture-based vaccine, and the skin is considered an attractive immunization site. In this study, we examined immunogenicity protective efficacy of influenza VLPs (H1N1 A/PR/8/34) after vaccination using vaccine dried on solid microneedle arrays. Coating microneedles with unstabilized formulation was found to decrease hemagglutinin (HA) activity, whereas inclusion trehalose disaccharide preserved HA activity VLP vaccines were coated....
Background. Respiratory syncytial virus (RSV) is the leading cause of bronchiolitis and viral death in infants. Despite decades research with traditional or subunit vaccine approaches, there are no approved RSV vaccines. New approaches therefore urgently needed to develop effective Methods. We developed viruslike particles (VLPs) consisting an influenza matrix (M1) protein core RSV-F -G on surface. tested immunogenicity efficacy these VLPs (RSV-F, RSV-G) a mouse model. Results. Intramuscular...
Abstract Aerosolized pathogens are a leading cause of respiratory infection and transmission. Currently used protective measures pose potential risk primary/secondary Here, we report the development universal, reusable virus deactivation system by functionalization main fibrous filtration unit surgical mask with sodium chloride salt. The salt coating on fiber surface dissolves upon exposure to aerosols recrystallizes during drying, destroying pathogens. When tested tightly sealed sides,...
Recovery from live influenza virus infection is known to induce heterosubtypic immunity. In contrast, immunity induced by inactivated vaccines predominantly subtype specific. this study, we investigated the protective virus. Intranasal immunization of mice with A/PR8 (H1N1) provided complete protection against homologous and a drift within same subtype, A/WSN (H1N1), but not A/Philippines (H3N2). However, coadministration cholera toxin as an adjuvant conferred protection, without observed...
ABSTRACT We have designed a membrane-anchored form of the Toll-like receptor 5 ligand flagellin, major proinflammatory determinant enteropathogenic Salmonella , which was found to be glycosylated and expressed on cell surfaces. A chimeric influenza virus-like particle (cVLP) vaccine candidate containing A/PR8/34 (H 1 N ) hemagglutinin (HA), matrix protein (M1), modified flagellin as molecular adjuvant produced. The immunogenicity, including serum antibody levels cellular immune responses,...
Morbidity and mortality due to influenza could be reduced by improved vaccination.To develop a novel skin delivery method that is simple allows for easy self-administration, we prepared microneedle patches with stabilized vaccine investigated their protective immune responses.Mice vaccinated single dose of trehalose-stabilized developed strong antibody responses were long-lived. Compared traditional intramuscular vaccination, vaccination was superior in inducing immunity, as evidenced...
Recurrent outbreaks of highly pathogenic H5N1 avian influenza virus pose a threat eventually causing pandemic. Early vaccination the population would be single most effective measure for control an emerging pandemic.Influenza virus-like particles (VLPs) produced in insect cell-culture substrates do not depend on availability fertile eggs vaccine manufacturing. We VLPs containing A/Viet Nam1203/04 (H5N1) hemagglutinin, neuraminidase, and matrix proteins, investigated their preclinical...
The 2009 influenza pandemic and shortages in vaccine supplies worldwide underscore the need for new approaches to develop more effective vaccines.We generated virus-like particles (VLPs) containing proteins derived from A/California/04/2009 virus, tested their efficacy as a mice. A single intramuscular vaccination with VLPs provided complete protection against lethal challenge virus partial A/PR/8/1934 an antigenically distant human isolate. VLP induced predominant IgG2a antibody responses,...
Simple and effective vaccine administration is particularly important for annually recommended influenza vaccination. We hypothesized that delivery to the skin using a patch containing vaccine-coated microneedles could be an attractive approach improve vaccination compliance efficacy.Solid microneedle arrays coated with inactivated were prepared simple skin. However, stability of vaccine, as measured by hemagglutination activity, was found significantly damaged during coating. The addition...
Ginseng polysaccharide has been known to have multiple immunomodulatory effects. In this study, we investigated whether Panax ginseng (GP) would a preventive effect on influenza infection. Administration of mice with GP prior infection was found confer survival benefit against H1N1 (A/PR/8/34) and H3N2 (A/Philippines/82) viruses. Mice infected the 2009 virus suspended in solution showed moderately enhanced rates lower levels lung viral titers inflammatory cytokine (IL-6). Daily treatment...
Respiratory syncytial virus (RSV) causes severe acute lower respiratory tract disease. Retinoic acid-inducible gene-I (RIG-I) serves as an innate immune sensor and triggers antiviral responses upon recognizing viral infections including RSV. Since tripartite motif-containing protein 25 (TRIM25)-mediated K63-polyubiquitination is crucial for RIG-I activation, several viruses target initial activation through ubiquitination. RSV NS1 NS2 have been shown to interfere with RIG-I-mediated...