A5054700088- CRISPR and Genetic Engineering
- Gene Regulatory Network Analysis
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- CAR-T cell therapy research
- Microbial Metabolic Engineering and Bioproduction
- Mycotoxins in Agriculture and Food
- Pancreatic function and diabetes
- 3D Printing in Biomedical Research
- Neuroscience and Neural Engineering
- bioluminescence and chemiluminescence research
- Pluripotent Stem Cells Research
- RNA regulation and disease
- Wheat and Barley Genetics and Pathology
- Protein purification and stability
- Bacteriophages and microbial interactions
- MicroRNA in disease regulation
- Light effects on plants
- Carcinogens and Genotoxicity Assessment
- Photochromic and Fluorescence Chemistry
- DNA Repair Mechanisms
- PARP inhibition in cancer therapy
Roche Pharma AG (Germany)
2020-2024
ETH Zurich
2011-2022
Board of the Swiss Federal Institutes of Technology
2012
University of Konstanz
2010-2012
Stockholm University
2010
Exosomes are cell-derived nanovesicles (50-150 nm), which mediate intercellular communication, and candidate therapeutic agents. However, inefficiency of exosomal message transfer, such as mRNA, lack methods to create designer exosomes have hampered their development into interventions. Here, we report a set EXOsomal transfer cells (EXOtic) devices that enable efficient, customizable production in engineered mammalian cells. These genetically encoded exosome producer enhance production,...
The possibility to externally control gene expression is of fundamental importance in both basic and applied life sciences. Although there are some techniques available regulate mammalian cells, they rely on the presence ligand-sensing transcription factors, making it necessary generate cell lines or organisms that stably express these regulatory factors. In recent years, mechanisms relying direct RNA-ligand interactions for controlling have been discovered nature engineered artificially....
Allergic disorders are markedly increasing in industrialized countries. The identification of compounds that trigger the immunoglobulin E-dependent allergic reaction remains key to limit patients' exposure critical allergens and improve their quality life. Here we use synthetic biology principles design a mammalian cell-based allergy profiler scores allergen-triggered release histamine from whole-blood-derived human basophils. A signalling cascade engineered within rewires input production...
Aptamers binding proteins or small molecules have been shown to be versatile and powerful building blocks for the construction of artificial genetic switches. In this study, we present a novel aptamer-based construct regulating Tet Off system in tetracycline-independent manner thus achieving control transgene expression. For purpose, TetR protein-inhibiting aptamer was engineered use mammalian cells, enabling RNA-responsive tetracycline-dependent transactivator (tTA). By rationally attaching...
Hammerhead ribozymes are self-cleaving RNA molecules capable of regulating gene expression in living cells. Their cleavage performance is strongly influenced by intra-molecular loop–loop interactions, a feature not readily accessible through modern prediction algorithms. Ribozyme engineering and efficient implementation ribozyme-based genetic switches requires detailed knowledge individual self-cleavage performances. By rational design, we devised fluorescent aptamer-ribozyme architectures...
In cellular systems environmental and metabolic signals are integrated for the conditional control of gene expression. On other hand, artificial manipulation expression is high interest genetic engineering. Especially reprogramming patterns to orchestrate responses in a predictable fashion considered be great importance. Here we introduce highly modular RNA-based system performing Boolean logic computation at post-transcriptional level Escherichia coli. We have previously shown that...
Abstract Engineered mammalian cells are key for biotechnology by enabling broad applications ranging from in vitro model systems to therapeutic biofactories. cell lines exist as a population containing sub-lineages of clones that exhibit substantial genetic and phenotypic heterogeneity. There is still limited understanding the source this inter-clonal heterogeneity well its implications biotechnological applications. Here, we developed genomic barcoding strategy targeted integration...
Abstract Die synthetische Biologie befasst sich mit der gezielten Konstruktion von lebenden Biomaschinen aus standardisierten Komponenten, die vordefinierte Aufgaben in einer (selbst‐)kontrollierten Weise ausführen können. Unterschiedliche Forschungsstrategien und interdisziplinäre Ansätze werden verfolgt, um technische Prinzipien zu implementieren. “top‐down”‐Strategie greift auf enorme Diversität Natur zurück, natürliche Komponenten künstlich genetischen, metabolischen oder...
Complex therapeutic antibody formats, such as bispecifics (bsAbs) or cytokine fusions, may provide new treatment options in diverse disease areas. However, the manufacturing yield of these complex formats Chinese Hamster Ovary (CHO) cells is lower than monoclonal antibodies due to challenges expression levels and potential formation side products. To overcome limitations, we performed a clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein 9...
A monoclonal antibody specific to ochratoxin B (OTB) was employed for the development of an indirect competitive OTB-ELISA. The optimized OTB-ELISA resulted in a limit detection (LOD) OTB 3 µg/L (8 nM), quantification (LOQ) 3.7 (10 and 50% inhibitory concentration (IC(50)) 150 nM. Due very low cross-reactivity OTA (2.7%) structurally related molecules (0%), this found be suitable detect with excellent precision different matrices, i.e., beer, coffee wine. Therefore, will allow screening food...
Current protocols for generating stable transgenic cell lines mostly rely on antibiotic selection or the use of specialized lacking an essential part their metabolic machinery, but these approaches require working with either toxic chemicals knockout lines, which can reduce productivity. Since most mammalian cells cannot utilize cellobiose, a disaccharide consisting two β-1,4-linked glucose molecules, we designed antibiotic-free system, CelloSelect, consists cassette encoding Neurospora...