A5061290456- Cell Image Analysis Techniques
- T-cell and B-cell Immunology
- Advanced Fluorescence Microscopy Techniques
- Genetics, Aging, and Longevity in Model Organisms
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Biomarkers in Disease Mechanisms
- Cellular Mechanics and Interactions
- Dermatology and Skin Diseases
- Reproductive System and Pregnancy
- Prenatal Screening and Diagnostics
- Microtubule and mitosis dynamics
- Hematological disorders and diagnostics
- Gynecological conditions and treatments
- Image Processing Techniques and Applications
- Biocrusts and Microbial Ecology
- Photosynthetic Processes and Mechanisms
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Olfactory and Sensory Function Studies
- Cell Adhesion Molecules Research
- Algal biology and biofuel production
- Cellular transport and secretion
- Reproductive Biology and Fertility
- Endometriosis Research and Treatment
University of Oregon
2023-2024
University of California, San Francisco
2016-2021
University of California System
2021
Although much is known about the embryo during implantation, architecture of uterine environment in which early develops not well understood. We employed confocal imaging combination with 3D analysis to identify and quantify dynamic changes luminal structure murine uterus preparation for implantation. When applied mouse mutants implantation defects, this method detected striking peri-implantation abnormalities morphology that cannot be visualized by histology. revealed organization glands...
Radial meiotic initiation in the fetal ovary is patterned by Tex14-dependent germ cell bridges that hinder loss of Dppa3.
Intratumoral T cells that might otherwise control tumors are often identified in an "exhausted" state, defined by specific epigenetic modifications and upregulation of genes such as CD38, cytotoxic T-lymphocyte-associated protein 4 (CTLA4), programmed cell death 1 (PD1). Although the term imply inactivity, there has been little study this state at phenotypic level to understand extent their incapacitation. Starting with observation move more quickly through mouse longer they reside progress...
Abstract Multiphoton microscopy is a powerful technique for deep in vivo imaging scattering samples. However, it requires precise, sample-dependent increases excitation power with depth order to generate contrast tissue, while minimizing photobleaching and phototoxicity. We show here how adaptive can optimize illumination at each point 3D volume as function of the sample’s shape, without need specialized fluorescent labeling. Our method relies on training physics-based machine learning model...
The nucleolus, a membraneless organelle crucial for ribosome production, has unique nanoscale structure whose organization is responsive to cell signals and disease progression. Here, we highlight the potential of Expansion Microscopy (ExM) capturing intricate spatial functional information about organelles such as nucleolus nuclear foci. We apply dual protein (dual-proExM) in combination with click (click-ExM) capture images at highest resolution reported ∼45 ± 2 nm. Inhibition nucleolar...
Abstract Regulatory T cells (Tregs) reside in nonlymphoid tissues where they carry out unique functions. The molecular mechanisms responsible for Treg accumulation and maintenance these are relatively unknown. Using an unbiased discovery approach, we identified LAYN (layilin), a C-type lectin-like receptor, to be preferentially highly expressed on subset of activated Tregs healthy diseased human skin. Expression layilin was induced by TCR-mediated activation the presence IL-2 or TGF-β. Mice...
During C . elegans oocyte meiosis I cytokinesis and polar body extrusion, cortical actomyosin is locally remodeled to assemble a contractile ring that forms within remains part of much larger actively network. This network both mediates dynamics generates shallow ingressions throughout the cortex during extrusion. Based on our analysis requirements for CLS-2, member CLASP family proteins stabilize microtubules, we recently proposed balance actomyosin-mediated tension microtubule-mediated...
Abstract Intratumoral T cells that might otherwise control tumors are often identified in an ‘exhausted’ state, defined by specific epigenetic modifications as well upregulation of genes such CD38, CTLA-4 and PD-1. While the term imply inactivity, there has been little study this state at phenotypic level to understand extent their incapacitation. Starting with observation move more quickly through mouse residence time increases they progress towards exhaustion, we elaborated a...
Arp2/3 complex nucleates branched actin filaments that drive membrane invagination during endocytosis and leading-edge protrusion in lamellipodia. is maximally activated vitro by binding of a WASP family protein to two sites-one on the Arp3 subunit one spanning Arp2 ARPC1-but importance each site regulation force-producing networks unclear. Here, we identify mutations budding yeast decrease or block engagement Las17, WASP, at site. As mammalian system, both sites are required for maximal...
Multiphoton microscopy is a powerful technique for deep in vivo imaging scattering samples. However, it requires precise, sample-dependent increases excitation power with depth order to maintain signal while minimizing photodamage. We show that cells identical fluorescent labels imaged situ can be used train physics-based machine learning model solves this problem. After training has been performed, the correct illumination predicted and adaptively adjusted at each point 3D volume on...
A new cava filter has been tried in 23 dogs weighing between 16 and kg. The consists of a stainless steel basket which can be introduced either antegrade or retrograde through 10F teflon catheter removed again within the first four days. was effective holding back embolising thrombus. In early stages, even without artificial embolisation, two out five animals showed clot formation basket. After three months, all filters were permeable on only occasions small amount thrombus found.
Abstract During C. elegans oocyte meiosis I, cortical actomyosin is locally remodeled to assemble a contractile ring near the spindle. In contrast mitosis, when most converges into ring, small forms within and remains part of much larger actively network. This network both mediates dynamics generates shallow ingressions throughout cortex during polar body extrusion. Based on our analysis requirements for CLS-2, member CLASP family proteins that stabilize microtubules, we recently proposed...
Introduction: Acute exposure to tobacco or marijuana secondhand smoke (SHS) causes endothelial dysfunction. The identity of specific SHS constituents that cause vascular toxicity is unclear. Heavy metals are present in and at elevated levels the blood smokers, may mediate acute dysfunction through reactive oxygen species formation decreasing NO bioavailability. We assessed effects cadmium, lead, mercury, arsenic human smokers on flow-mediated dilation (FMD) as a measure function rats. also...