The use of botanical seeds shallot as planting materials is more effective than bulbs. However, the characteristics plants are not ‘true to type’. Bibliometric analysis can identify areas that have been under- explored. Research on biomolecule compounds and gene expression needed support biomarker-based detection technology predict plant productivity early. This research aims study AcFT1 compare two plantlets with different responses (non-multiplied multiplied). was identified by...

Since effective antiviral drugs for COVID-19 are still limited in number, the exploration of compounds that have activity against SARS-CoV-2 is high demand. Porphyrin potentially developed as a drug. However, its low solubility water restricts clinical application. Reconstruction porphyrin into carbon dots expected to possess better and bioavailability well lower biotoxicity. In this study, we investigated porphyrin-derived SARS-CoV-2. Through silico analysis assessment using novel drug...

AbstrakBakteri endofit merupakan mikroorganisme menguntungkan yang berinteraksi dengan tanaman inang tanpa menyebabkan gangguan atau kerusakan pada tanaman.Beberapa studi menunjukkan bahwa bakteri tertentu dapat memproduksi senyawa kimia memiliki efek bagi kesehatan, terutama diisolasi dari obat.Binahong (Anredera cordifolia) dan Ketepeng Cina (Cassia alata) obat telah diketahui beberapa khasiat.Tujuan penelitian ini ialah mengisolasi mengkarakterisasi kedua tersebut melalui uji...

Breast cancer is associated with an excessive function of somatic mutation in phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit α (PIK3CA), specifically exon 9 and 20 hotspots. Exon 20, which contains H1047R, has more potential for oncogenic than 9. Detection PIK3CA/H1047R also been reported molecular diagnosis therapeutic application. Sensitive methods are required because mutants mostly present at low levels a mixture the wild type. Therefore, this study aimed to explore...

The dataset presented in this article is related to the research entitled "Detection of HER2 Gene Polymorphism Breast Cancer: PCR Optimization Study" (B.R. Budiarto, Desriani, 2016) [1] with some modification primers used and optimization strategy eliminate false-positive result that may occur HER2I655V polymorphism detection. Combining a new set gradient, allele-specific well performs detect all type breast cancer-originated genotypes. validation method was done using Sanger DNA sequencing,...

Breast cancer is a disease in which cell grows rapidly forming mass the breast. HER2 polymorphisms Ile655Val have been studied as biomarkers for breast and may comprise risk factor of cardiac toxicity cancer-consuming trastuzumab. Aim work: In this study, we developed simple, low cost, rapid test to detect polymorphism at gene using SYBR Green I-based melting curve method. report, performed allelic discrimination with real-time temperature (Tm) Shift The profiles amplified DNA HER-2 its...

An enzyme fuel cell employing a carbon cloth electrode and bacterial FAD dependent glucose dehydrogenase (FADGDH) based on the direct electron transfer principle was constructed, its scalability cellulolytic sugar conversion were investigated.FADGDH immobilized together with paste to form multi-module type by combining platinum-supported as cathode.The stable for more than 7 days of continuous operation.The 3 module (18 cm 2 ) generated 68 μW (3.8 μW/cm using substrate, which almost 9 times...

Paddy var Rojoleleis asuperior paddy come from Klaten that released by Department of Agriculture in 2003. Its superior properties are resistant to pests leaf hoppers, fluffier, and fragrant. To increase the productivity ten used farmers is use chemical-based fertilizers. The these chemicals will effect adisruption ecosystem balancing, reduction amount soil microflora which essential forplants. Endophytic bacteria symbiotic microorganisms living within plant tissues, does not cause negative...

Bullying menjadi masalah serius dan sangat berbahaya pada usia sekolah. Cara untuk mengatasi bullying adalah dengan memberikan pendidikan kesehatan yaitu menggunakan metode ceramah. Penelitian ini bertujuan mengetahui pengaruh ceramah terhadap pengetahuan anak dilakukan di Sekolah Dasar Negeri 101 Pekanbaru selama 2 hari tanggal 19-20 juni 2019. Desain penelitian yang digunakan quasi experiment pre and post test without control. Populasi dari kelas 3-5 sampel berjumlah 177 siswa pengambilan...

The phosphatidylinositol 3-kinases (PIK3s) are lipid kinases. Mutation in the exon 9 and 20 determined as a predictive factor anti-HER-2 therapy. In some countries, such Singapore, China, Peru, PIK3CA E545A was reported to produce highest rate of mutation. this research, we developed optimized detection methods with intercalating dye SYBR Green I based on Tm Shift approach by using prepared recombinant plasmid pGEMT-easy E545A. Recombinant used due limited number samples.Recombinant...

Background: Currently available molecular method to detect HER2I655V polymorphism such as PCR-RFLP is hampered by the costly experimental method, and post-PCR treatment requirement that makes this technique not meeting for high-throughput analysis purpose. In study, we developed an accurate, simple, low cost rapid test at HER2 gene using SBR Green I based-melting curve method. Methods: Two forward allele-specific primers one common reverse primer were used then these tested discriminate...

The adulteration of processed beef-based meat products with pork is a sensitive issue in Indonesia. In this study, we developed detection method for the low cost identification products. We used cost-efficient Taq DNA polymerase, DreamTaq Green PCR master mix (2x), and duplex to recognize simultaneously 18S rRNA detection. A positive control containing gene inserted into pGEM®-T easy was prepared, along negative control. results were assess its specificity, limit, ability different food...

Penelitian ini bertujuan untuk mendapatkan informasi tentang bagaimana persepsi guru terhadap pelaksanaan supervisi oleh pengawas SMK Negeri di Kecamatan Lubuk Basung Kabupaten Agam dilihat dari persiapan, pelaksanaan, evaluasi dan tindak lanjut supervisi. Populasi penelitian adalah yang berjumlah 150 orang, penarikan sampel dilakukan dengan teknik proporsional random sampling sejumlah 75 orang. Data ambil menggunakan angket. Adapun hasil diperoleh rata-rata indikator persiapan (3,3)...

HER2 is a protein that essential to the process of differentiation, proliferation, tissue development and other physiological processes. HER-2 overexpression was occurred in 20-30% breast cancer patients. Targeted therapies performed using trastuzumab, on basis examination by immunohistochemistry. Single nucleotide polimorphisme (SNP) Ile655Val were found HER-2, which indicates risk as well predictive factor treatment with targeted therapies. The method had been used for SNP detection TaqMan...

Various kinds of culinary are rife in the middle city padangsidimpuan by using thr name processed food a strange name. one branded products thet currently becoming tren community meatballs with children, devil’s noodles, missile meatball cones and so on.Islam requires that to be consumed will guaranteed halal pure. Halal is not only from type but also where explanation MUI Fatwa Number 4 2003 concerning standardization fatwas, it contained fourth section: problem names ingredients point 1,...

A reliable method to detect gene polymorphisms must be established eliminate genotyping errors due false PCR amplification. In the previous study, we have developed AS-PCR (Allele Specific-Polymerase Chain Reaction) HER2 Ile655Val polymorphism with good specificity and sensitivity, yet it produces some errors. This study is aimed source of mainly by betaine treatment program modification. Genotyping produced was qualitatively quantitatively evaluated using two genomic DNA that each contained...

DNA recombinant technology is a tool that could support thedevelopment of pharmacogenomic detection such as for CYP2D6 copy number application. The housekeeping gene was identified an internal control alternative the component. There are many kinds genes, including albumin. In this report, we have successfully TA-cloned partial albumin to development detection. TA-cloning confirmed with white-blue methods in media LB + ampicillin IPTG X-Gal and colony PCR methods. Further, made standard...

<ns3:p>Background Cytochrome P450 2D6 (CYP2D6) is an important enzyme that metabolizes commonly used drugs, such as tramadol hydrochloride (a widely opioid analgesic). Genetic polymorphisms of CYP2D6 have been shown to influence the pharmacodynamic properties administered drugs. This study aimed screen 63 postoperative patients (wild-type, CYP2D6*5, and multiplication) Minangkabau ethnicity in West Sumatera, Indonesia, who received using a modified long PCR method, investigate clinical...

Abstract Dadiah is a traditional spontaneous fermentation of buffalo milk from West Sumatra that doesn’t go through pasteurized stages. The aim the study to find out biodiversity α and β diversity microbiota in level genus dadiah. V3 -V4 hypervariable region 16 S rRNA deep sequencing was used detected bacteria. available data were analyzed by Alpha Diversity (richness evenness) Beta (Taxonomy Chart Heatmaps). These studies revealed most divergent sample appropriate C3 with Shanon Index...

<ns3:p><ns3:bold>Background:</ns3:bold> Mesenchymal stem cells (MSCs) can differentiate into nerve with an induction from chemical compounds in medium culture. <ns3:italic>Chromolaena odorata</ns3:italic> contains active compounds, such as alkaloids and flavonoids, that initiate the transformation of MSCs cells. The aim this study was to determine potential methanol extracted <ns3:italic>C. leaf induce differentiation bone marrow cells.</ns3:p><ns3:p> <ns3:bold>Methods:</ns3:bold> A serial...

Rismaya, Azamris, Budiarti S, Desriani. 2017. Housekeeping gene as a source of calibrator candidate for HER-2 scoring in frozen tissue breast cancer study based on qPCR. Biodiversitas 18: 1041-1046. amplification gives an implication therapy and prognosis. FDA have been approved Fluorescence In situ hybridization (FISH) immunohistochemistry (IHC) protein expression measurement. this recently times, quantitative PCR techniques reported alternative method determination. Here report, we...