A5101951291- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Malaria Research and Control
- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- Respiratory viral infections research
- Biosensors and Analytical Detection
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Bacteriophages and microbial interactions
- Animal Virus Infections Studies
- SARS-CoV-2 detection and testing
- Bacillus and Francisella bacterial research
- Herpesvirus Infections and Treatments
- Plant Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Identification and Quantification in Food
- Viral Infections and Immunology Research
- Genomics and Phylogenetic Studies
- interferon and immune responses
- Poxvirus research and outbreaks
- Extracellular vesicles in disease
- Machine Learning in Bioinformatics
Defence Research and Development Establishment
2013-2023
Indian Veterinary Research Institute
2020
To investigate the aetiology of 2015 A(H1N1)pdm09 influenza outbreak in India, 1,083 nasopharyngeal swabs from suspect patients were screened for state Madhya Pradesh. Of 412 positive specimens, six further characterised by phylogenetic analysis haemagglutinin (HA) sequences revealing that they belonged to genogroup 6B. A new mutation (E164G) was observed HA2 two sequences. Neuraminidase genes 12 isolates fatal cases on prior oseltamivir treatment harboured H275Y mutation.
The resurgence of Chikungunya (CHIK) virus in the form an explosive, unprecedented epidemic with high virulence and unusual numbers fatalities has created immense public health concern recent years. In absence effective vaccine specific antiviral therapy, early accurate diagnosis is essential for best patient management. present study describes production characterization high-affinity selective monoclonal antibodies (Mabs) against recombinant E2 protein (rE2) CHIK virus. reactivity Mabs rE2...
Despite the fact that Chikungunya resurgence is associated with epidemic of unprecedented magnitude, there are challenges in field its clinical diagnosis. However, serological tests an ELISA format provide a rapid tool for diagnosis infection. Indeed, ELISAs based on recombinant proteins hold great promise as these methods cost effective and free from risk handling biohazardous material. In this study, performance CHIKV antigens was compared various formats Chikungunya. Two derived envelope...
Burkholderia mallei is the aetiological agent of glanders, a highly contagious and re-emerging zoonotic disease. Early diagnosis glanders critically important to ensure timely treatment with appropriate antibiotics in humans, prevent spread infection animals. Molecular detection B. has always been troublesome because its genetic similarity pseudomallei, causative melioidosis. In present investigation, set six mallei-specific primers were designed simple, rapid, specific sensitive real-time...
Abstract An early diagnosis of Japanese encephalitis (JE) is important for timely clinical management and epidemiological control in areas where multiple flaviviruses are endemic. The NS1 antigen has an advantage over IgM enzyme‐linked immunosorbent assay (ELISA) confirmatory virus (JEV) infection due to its proliferation on the surface host cells acute phase infection. In this study, development evaluation JE‐specific capture ELISA described using high‐affinity monoclonal antibody specific...
Background Influenza A virus is one of world’s major uncontrolled pathogen, causing seasonal epidemic as well global pandemic. This was evidenced by recent emergence and continued prevalent 2009 swine origin pandemic H1N1 virus, provoking first true in the past 40 years. In course its evolution, acquired many mutations multiple unidentified molecular determinants are likely responsible for ability to cause increased disease severity humans. Availability limited data on complete genome...
Crimean-Congo hemorrhagic fever (CCHF) is a re-emerging zoonotic viral disease prevalent in many parts of Asia, Europe and Africa. The causative agent, Orthonairovirus (CCHFV) transmitted through hard ticks. Tick vectors especially belonging to Hyalomma species serve as the reservoir amplifying host. vertebrate animals including sheep, goat bovine act short lasting bridge linking virus . CCHFV causes fatal humans. Humans are usually infected with either bite ticks or by close contact...
Abstract Crimean-Congo hemorrhagic fever (CCHF) virus, a highly pathogenic viral agent is responsible for severe fatal infections in many parts of the world. The early diagnosis CCHF infection important successful clinical management and epidemiological control. nucleoprotein (NP) CCHFV being conserved immunogenic used as diagnostic marker. In this study, we report rapid sensitive double antibody based antigen capture ELISA to detect virus (CCHFV). Highly specific polyclonal monoclonal...
Mpox (previously known as Monkeypox) has recently re-emerged, primarily through human-to-human transmission in non-endemic countries including India. Virus isolation is still considered the gold standard for diagnosis of viral infections. Here, qPCR positive skin lesion sample from a patient was inoculated Vero E6 cell monolayer. Characteristic cytopathic effect exhibiting typical rounding and detachment observed at passage-02. The virus confirmed by qPCR. replication kinetics isolate...
West Nile virus (WNV) is a mosquito-borne flavivirus. The disease can be diagnosed by isolation followed fluorescent antibody tests, enzyme-linked immunosorbent assay and polymerase chain reaction (PCR) assay. These diagnostic methods are laborious time-consuming. present study was aimed to evaluate the real-time reverse-transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid, early accurate diagnosis of WNV.A one-step single tube accelerated quantitative RT-LAMP...
West Nile virus (WNV) is a mosquito-borne of public health importance. Currently, there no FDA approved vaccine available against WNV infection in humans. Therefore, the early diagnosis important for epidemiologic control and timely clinical management areas where multiple Flaviviruses are endemic. The present study aimed to develop reverse transcription polymerase spiral reaction (RT-PSR) assay that rapidly accurately detects envelope (env) gene WNV. RT-PSR was optimized at 63°C 60 min...
Summary Japanese encephalitis is a major public health problem in South‐East Asia and Western Pacific countries. The recombinant nonstructural 1 (rNS1) protein of virus potential diagnostic as well vaccine candidate. Developments cost‐effective simple culture media appropriate conditions are generally favourable for large‐scale production proteins. effects medium composition cultivation on the rNS1 were investigated shake flask batch Escherichia coli . Further, fed‐batch process was also...
Background: The West Nile Virus (WNV) has emerged as one of the most significant arboviral infection in many parts world and is associated with encephalitis affecting mainly human horses. In spite fact that WNV threat for public health, there no vaccine or therapeutic available treatment WNV. Methods: this study, we tested a novel RNA interference based technique to inhibit replication Vero cells. Two siRNAs were designed against NS2A NS5 regions which are highly conserved among Flaviviruses...
Background: Duck plague is a highly contagious viral disease reported in our country very often with significant economic loss. There are some bottlenecks the currently used ‘Holland strain’ vaccine that involves cumbersome process of production embryonated chicken eggs. With future goal development an indigenous cell culture for duck plague, present study aimed at isolation Indian strain DEV from natural outbreak and its characterization seed virus purpose. Methods: Liver samples were...
West Nile encephalitis caused by infection with the virus (WNV) is endemic in many regions of world and a global public health threat. The aim this report was to develop method using colorimetry-based reverse-transcription loop-mediated isothermal amplification (cRT-LAMP) RT-LAMP combined lateral-flow dipstick (LFD) for rapidly detecting WNV low-infrastructure settings.The primers cRT-LAMP RT-LAMP-LFD assays were designed based on env gene WNV. Primers concentration, temperature time...
Five complete (H1N1)pdm09 viral sequences were recovered from hospitalized individuals during the 2015 influenza outbreak by metagenomic sequencing. Four of genomes are oropharyngeal swabs, and one is an isolate.
Kyasanur forest disease (KFD), a re-emerging tick-borne viral disease, causes severe hemorrhagic fever in humans and nonhuman primates. KFD virus (KFDV) is member of the genus Flavivirus. now increasingly reported outside its endemic zone India. Rapid specific detection KFDV plays critical role containment outbreak. The diagnosis currently relies on real-time RT-PCR, nested end point serodiagnostic assay. These assays are tedious, time-consuming, cannot be used as routine screening...