A5003887328- Cannabis and Cannabinoid Research
- Eicosanoids and Hypertension Pharmacology
- Photosynthetic Processes and Mechanisms
- Alcohol Consumption and Health Effects
- Advanced Electron Microscopy Techniques and Applications
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Pharmacogenetics and Drug Metabolism
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Neuroscience of respiration and sleep
- RNA modifications and cancer
- Biochemical effects in animals
- Cognitive and developmental aspects of mathematical skills
- Peroxisome Proliferator-Activated Receptors
- Biochemical Acid Research Studies
- Metabolism and Genetic Disorders
- Force Microscopy Techniques and Applications
- Reading and Literacy Development
- Forensic Toxicology and Drug Analysis
- Genomics, phytochemicals, and oxidative stress
- Receptor Mechanisms and Signaling
- Spectroscopy and Quantum Chemical Studies
- Molecular Junctions and Nanostructures
- Neurotransmitter Receptor Influence on Behavior
- Education and Technology Integration
Scripps Research Institute
2023-2025
University of Illinois Urbana-Champaign
2018-2024
St. John Fisher College
2012
The antioxidant/anti-inflammatory compound carnosic acid (CA) is a phenolic diterpene found in the herbs rosemary and sage. Upon activation, CA manifests electrophilic properties to stimulate Nrf2 transcriptional pathway via reaction with Keap1. However, purified readily oxidized thus highly unstable. To develop as an Alzheimer's disease (AD) therapeutic, we synthesized pro-drug derivatives, among which di-acetylated form (diAcCA) showed excellent drug-like properties. diAcCA converted...
Biological phenomena, from enzymatic catalysis to synaptic transmission, originate in the structural transformations of biomolecules and biomolecular assemblies liquid water. However, directly imaging these nanoscopic dynamics without probes or labels has been a fundamental methodological challenge. Here, we developed an approach for “electron videography”—combining phase electron microscopy with molecular modeling—with which filmed nanoscale fluctuations individual, suspended, unlabeled...
Abstract The endocannabinoid system is a promising target to mitigate pain as the endocannabinoids are endogenous ligands of pain-mediating receptors—cannabinoid receptors 1 and 2 (CB1 CB2) TRPV1. Herein, we report on class lipids formed by epoxidation N-arachidonoyl-dopamine (NADA) N-arachidonoyl-serotonin (NA5HT) epoxygenases. EpoNADA epoNA5HT dual-functional rheostat modulators endocannabinoid-TRPV1 axis. stronger TRPV1 than either NADA or NA5HT, displays significantly inhibition...
The human body contains endogenous cannabinoids (endocannabinoids) that elicit effects similar to those of Δ9-tetrahydrocanabinol, the principal bioactive component cannabis. endocannabinoid virodhamine (O-AEA) is constitutional isomer well-characterized cardioprotective and anti-inflammatory anandamide (AEA). chemical structures O-AEA AEA contain arachidonic acid (AA) ethanolamine; however, AA in connected ethanolamine via an ester linkage, whereas through amide linkage. involved regulating...
Omega-3 fatty acid derived endocannabinoids are metabolized by cytochrome P450s to form bioactive endocannabinoid epoxides that anti-inflammatory Lipidomics, LC-MS/MS, microglial cells culture, lipid synthesis, extractions, enzymology Cannabinoids found in marijuana and also produced naturally the body from ω-3 ω-6 acids. Exocannabinoids marijuana, known be responsible for some of its euphoric effects, but they exhibit benefits. Our study revealed a cascade enzymatic reactions convert acids...
Prevention of infection and propagation SARS-CoV-2 is high priority in the COVID-19 pandemic. Here, we describe S-nitrosylation multiple proteins involved infection, including angiotensin converting enzyme 2 (ACE2), receptor for viral entry. This reaction prevents binding ACE2 to Spike protein, thereby inhibiting entry, infectivity, cytotoxicity. Aminoadamantane compounds also inhibit coronavirus ion channels formed by envelope (E) protein. Accordingly, developed dual-mechanism...
Journal Article Real-time Electron Microscopy of Protein Nanodiscs in Graphene Liquid Cells Get access John W Smith, Smith Department Materials Science and Engineering, University Illinois, Urbana, IL 61801, United States Search for other works by this author on: Oxford Academic Google Scholar Lauren N Carnevale, Carnevale Biochemistry, Aditi Das, Das StatesDepartment Comparative Biosciences College Veterinary Medicine, Qian Chen Chemistry, Beckman Institute Advanced Technology, Research...
Biological phenomena ranging from enzymatic catalysis to synaptic transmission find their origins in the morphological fluctuations of biomolecules and biomolecular assemblies liquid water. However, directly imaging these nanoscopic dynamics without probes or labels has been a fundamental methodological challenge. Here we developed an approach for ‘molecular videography’—combining liquid-phase electron microscopy with molecular modelling—with which filmed nanoscale individual, freely...
ABSTRACT The endocannabinoid (eCB) system is a promising target to mitigate pain as the eCBs are endogenous ligands of pain-mediating receptors—cannabinoid receptors 1 and 2 (CB1 CB2) TRPV1. Here we report on novel class lipids formed by epoxidation N-arachidonoyl-dopamine (NADA) N-arachidonoyl serotonin (NA5HT) cytochrome P450 (CYP) epoxygenases. These epoxides (epoNADA epoNA5HT) dual-functional rheostat modulators (varying strength agonism or antagonism) eCB-TRPV1 axis. In fact, epoNADA...