A5006784178- 3D Printing in Biomedical Research
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Osteoarthritis Treatment and Mechanisms
- Silk-based biomaterials and applications
- Angiogenesis and VEGF in Cancer
- Adipose Tissue and Metabolism
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced Cellulose Research Studies
- Nasal Surgery and Airway Studies
- Bone fractures and treatments
- Additive Manufacturing and 3D Printing Technologies
- Reconstructive Surgery and Microvascular Techniques
- Adipokines, Inflammation, and Metabolic Diseases
- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- Fatty Acid Research and Health
- Advanced Breast Cancer Therapies
- Bone Tissue Engineering Materials
- Wound Healing and Treatments
- Trauma and Emergency Care Studies
Sahlgrenska University Hospital
2006-2023
University of Gothenburg
2018-2023
Region Västra Götaland
2019-2023
University Hospital Ulm
2017
Background: The three-dimensional (3D) bioprinting technology allows creation of 3D constructs in a layer-by-layer fashion utilizing biologically relevant materials such as biopolymers and cells. aim this study is to investigate the use clinically setting evaluate potential technique for vivo chondrogenesis. Methods: Thirty-six nude mice (Balb-C, female) received 5- × 1-mm piece bioprinted cell-laden nanofibrillated cellulose/alginate construct subcutaneous pocket. Four groups printed were...
Cartilage repair and replacement is a major challenge in plastic reconstructive surgery. The development of process capable creating patient-specific cartilage framework would be breakthrough. Here, we described methods for human vivo quantitatively assessing the proliferative capacity cartilage-formation ability mono- co-cultures chondrocytes mesenchymal stem cells three-dimensional (3D)-bioprinted hydrogel scaffold. 3D-bioprinted constructs (5 × 5 1.2 mm) were produced using...
Bacterial nanocellulose (BNC) is a 3D network of nanofibrils exhibiting excellent biocompatibility. Here, we present the aqueous counter collision (ACC) method BNC disassembly to create bioink with suitable properties for cartilage-specific 3D-bioprinting. was disentangled by ACC, and fibril characteristics were analyzed. Bioink printing fidelity shear-thinning evaluated. Cell-laden bioprinted grid constructs (5 × 5 1 mm3) containing human nasal chondrocytes (10 M mL–1) implanted in nude...
Extracellular matrix fibril components, such as collagen, are crucial for the structural properties of several tissues and organs. Tunicate-derived cellulose nanofibrils (TNC) combined with living cells could become next gold standard cartilage soft-tissue repair, TNC fibrils present similar dimensions to feasible industrial production, chemically straightforward cost-efficient extraction procedures. In this study, we characterized physical derived from aquaculture production in Norwegian...
Three-dimensional (3D) bioprinting of cartilage is a promising new technique. To produce, for example, an auricle with good shape, the printed needs to be covered skin that can grow on surface construct. Our primary question was analyze if integrated 3D bioprinted structure tissue serve as bed full-thickness graft.3D constructs (10 × 10 1.2 mm) were using nanofibrillated cellulose/alginate bioink mixed mesenchymal stem cells and adult chondrocytes implanted subcutaneously in 21 nude...
Long-term stability and biological safety are crucial for translation of 3D-bioprinting technology into clinical applications. Here, we addressed the long-term issues associated with 3D-bioprinted constructs comprising a cellulose scaffold human cells (chondrocytes stem cells) over period 10 months in nude mice. Our findings showed that increasing unconfined compression strength time significantly improved mechanical cell-containing relative to cell-free scaffolds. Additionally, exhibited...
Establishing functional circulation in bioengineered tissue after implantation is vital for the delivery of oxygen and nutrients to cells. Native cartilage avascular thrives on diffusion, which turn depends proximity circulation. Here, we investigate whether a gridded three-dimensional (3D) bioprinted construct would allow ingrowth blood vessels thus prove concept vascularization tissue. Twenty 10 × 3-mm 3Dbioprinted nanocellulose constructs containing human nasal chondrocytes or cell-free...
Adipose tissue inflammation drives obesity-related cardiometabolic diseases. Enhancing endogenous resolution mechanisms through administration of lipoxin A4, a specialized pro-resolving lipid mediator, was shown to reduce adipose and subsequently protects against obesity-induced systemic disease in mice. Here, we demonstrate that lipoxins 3D-cultured human adipocytes explants from obese patients. Approximately 50% patients responded particularly well by reducing inflammatory cytokines...
The Rai and Binet staging systems are currently being challenged by the development of new biological methods to characterize prognosis management chronic lymphocytic leukemia (CLL). To evaluate these two in recently diagnosed CLL patients, we performed a retrospective population-based study including 344 patients western Sweden between 1995 2000. stage A had longer median overall survival (OS) (100 months) than B (55 months; P < 0.001) C (45 0.0005). Median OS for could not be separated (P...
Three-dimensional (3D)-bioprinted lipoaspirate-derived adipose tissue (LAT) is a potential alternative to lipo-injection for correcting soft-tissue defects. This study investigated the long-term
Establishing a vascular network in biofabricated tissue grafts is essential for ensuring graft survival. Such networks are dependent on the ability of scaffold material to facilitate endothelial cell adhesion; however, clinical translation potential tissue-engineered scaffolds hindered by lack available autologous sources cells. Here, we present novel approach achieving endothelialisation nanocellulose-based using adipose tissue-derived cells scaffolds. We used sodium periodate-mediated...
Autologous fat grafting is hampered by unpredictable outcomes due to high tissue resorption. Hydrogels based on enzymatically pretreated tunicate nanocellulose (ETC) and alginate (ALG) are biocompatible, safe, present physiochemical properties capable of promoting cell survival. Here, we compared in situ ex crosslinking ETC/ALG hydrogels combined with lipoaspirate human adipose (LAT) generate an injectable formulation retaining dimensional stability vivo. We performed using two different...