- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Microbial bioremediation and biosurfactants
- Bacterial Genetics and Biotechnology
- Legume Nitrogen Fixing Symbiosis
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- bioluminescence and chemiluminescence research
- CRISPR and Genetic Engineering
- DNA Repair Mechanisms
- Biochemical and Molecular Research
- Mycorrhizal Fungi and Plant Interactions
- Berberine and alkaloids research
- Carcinogens and Genotoxicity Assessment
- Botulinum Toxin and Related Neurological Disorders
- Nematode management and characterization studies
- RNA Interference and Gene Delivery
- Agricultural and Rural Development Research
- RNA and protein synthesis mechanisms
- Microbial Fuel Cells and Bioremediation
- Antibiotics Pharmacokinetics and Efficacy
- Pesticide and Herbicide Environmental Studies
- Effects and risks of endocrine disrupting chemicals
- Plant Pathogenic Bacteria Studies
- Diagnosis and Treatment of Venous Diseases
Gebze Technical University
2012-2022
Sabancı Üniversitesi
2022
Centro Nacional de Biotecnología
2018-2021
Afyon Kocatepe University
2014
During evolution of biodegradation pathways for xenobiotic compounds involving Rieske nonheme iron oxygenases, the transition toward novel substrates is frequently associated with faulty reactions. Such events release reactive oxygen species (ROS), which are endowed high mutagenic potential. In this study, we evaluated how operation background metabolic network by an environmental bacterium may either foster or curtail still-evolving pathway 2,4-dinitrotoluene (2,4-DNT) catabolism. To end,...
The molecular mechanisms behind the mutagenic effect of reactive oxygen species (ROS) released by defective metabolization xenobiotic 2,4-dinitrotoluene (DNT) a still-evolving degradation pathway were studied. To this end, genes required for biodegradation DNT from Burkholderia cepacia R34 implanted in Escherichia coli and catabolizing nitroaromatic compound monitored with stress-related markers reporters. Such proxy naturally-occurring scenario faithfully recreated known accumulation ROS...
The CRISPR-Cas 9 system, which is known as a natural way of bacteria to defend against phage infection and plasmid transfer, has been re-purposed RNA-guided DNA targeting strategy for genome editing. Together with the advances gained in sequencing technology, this platform opened new era molecular biology since its recognition was specified by 20-nt single-guide RNA made technique easier, efficient simple application any organism. Thus, many studies have discussed performed applications...
The aim of this study was to analyze possible positive effects putatively endophytic PGPB (PGB_invit), which isolated from long-term in vitro cultured fraser photinia microshoots, on seed and 7-day old seedling stages Arabidopsis thaliana. Seeds vitro-germinated seedlings were inoculated with 107 CFU/mL 108 active (A) inactive (I) bacterial populations along their mix compositions (A+I) suspended MPYE broth together controls (untreated ones). 14 days evaluated for various plant growth...
ABSTRACT During evolution of biodegradation pathways for xenobiotic compounds, the transition towards novel substrates Rieske non-heme iron oxygenases borne by environmental bacteria is frequently associated with faulty reactions. Such reactions release reactive oxygen species (ROS), endowed high mutagenic potential. The present work studies how operation a given metabolic network bacterial host may either foster or curtail still-evolving biochemical pathway catabolism 2,4-dinitrotoluene...
Searchable abstracts of presentations at key conferences on reproductive biology and medicine ISSN 2052-1472 (online)
A. thaliana genomu dizilenmiş genetik, biyokimyasal ve biyoteknolojik çalışmalarda yaygın olarak kullanılan model bir bitkidir. P. putida bakterisi ise bitki köklerinde kolonize olabilen, dizilenmiş, büyümesini teşvik eden, patojenik olmayan bitki-bakteri etkileşimlerinin anlaşılması için olmaya aday rizosferik bakteridir. Bu nedenle, bu çalışmanın amacı thaliana’nın in vitro çimlenmesi fideciklerinin büyümesi üzerine putida’nın etkisinin değerlendirilmesidir. amaçla, yüzey sterilasyonu...
Abstract An endophytic beneficial bacterium, was isolated and characterized from in vitro grown microshoots of Fraser’s Photinia our previous studies. This nominated as PGB_invit ( Pseudorhodoplanes sp.), has ability to fix nitrogen produce plant growth promoting regulators such indole acetic acid gibberellic acid. Probably because all these features, this specific bacterium promotes proliferation enables be conserved at 25 ºC for up 16 months without requiring periodic subculture. Due its...
Despite its environmental robustness Pseudomonas putida strain KT2440 is very sensitive to DNA damage and displays poor homologous recombination efficiencies. To gain an insight into this deficiency isogenic ∆recA ∆lexA1 derivatives of prophage-free P. EM173 were generated responses the recA lexA1 promoters tested with GFP reporter technology. Basal expression high in absence only moderately induced by norfloxacin. A similar behaviour was observed when equivalent fusions lexA E. coli placed...