A5004111784- Respiratory Support and Mechanisms
- Sepsis Diagnosis and Treatment
- Cardiac, Anesthesia and Surgical Outcomes
- Intensive Care Unit Cognitive Disorders
- Cardiac Arrest and Resuscitation
- Blood transfusion and management
- Hemodynamic Monitoring and Therapy
- Family and Patient Care in Intensive Care Units
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Anesthesia and Sedative Agents
- Inhalation and Respiratory Drug Delivery
- Neonatal Respiratory Health Research
- Palliative Care and End-of-Life Issues
- Nosocomial Infections in ICU
- Patient Safety and Medication Errors
- Renal function and acid-base balance
- Mechanical Circulatory Support Devices
- Anesthesia and Neurotoxicity Research
- Milk Quality and Mastitis in Dairy Cows
- Interprofessional Education and Collaboration
- Healthcare Policy and Management
- Healthcare cost, quality, practices
- Airway Management and Intubation Techniques
- Clinical Reasoning and Diagnostic Skills
- Hemoglobin structure and function
University of California, San Francisco
2014-2025
Association of Perioperative Registered Nurses
2016-2020
University of Toronto
2016-2017
University Health Network
2016
Mount Sinai Hospital
2016
University of Hawaiʻi at Mānoa
2016
University of California San Francisco Medical Center
2013
American Society of Anesthesiologists
2013
May Institute
2010
University of California, San Diego
2009
Two water channel homologs were cloned recently from rat kidney, mercurial-insensitive (MIWC) and glycerol intrinsic protein (GLIP). Polyclonal antibodies raised against synthetic C-terminal peptides purified by affinity chromatography. MIWC GLIP recognized proteins in kidney with an apparent molecular mass of 30 27 kDa, respectively, did not cross-react. By immunofluorescence, expressed together on the basolateral plasma membrane collecting duct principal cells kidney. immunohistochemistry,...
ABSTRACT It was shown recently that water channel homologs MIWC (mercurial insensitive channel) and GLIP (glycerol intrinsic protein) colocalized in basolateral membranes of kidney collecting duct, tracheal colonic epithelia, brain pia mater. We report here an extensive immunolocalization study non-epithelial glandular epithelial tissues rat. Immunogold electron microscopy confirmed colocalization membrane principal cells duct. However, other but not expressed parietal stomach, excretory...
To provide a focused, detailed assessment of the symptom experiences intensive care unit patients at high risk dying and to evaluate relationship between delirium patients' reports.Prospective, observational study symptoms.Two units in tertiary medical center western United States.One hundred seventy-one dying.None.Patients were interviewed every other day for up 14 days. Patients rated presence, intensity (1 = mild; 2 moderate; 3 severe), distress not very distressing; moderately...
Guidelines currently recommend targeting light sedation with dexmedetomidine or propofol for adults receiving mechanical ventilation. Differences exist between these sedatives in arousability, immunity, and inflammation. Whether they affect outcomes differentially mechanically ventilated sepsis undergoing is unknown.In a multicenter, double-blind trial, we randomly assigned to receive (0.2 1.5 μg per kilogram of body weight hour) (5 50 minute), doses adjusted by bedside nurses achieve target...
The pathogenesis of septic shock occurring after Pseudomonas aeruginosa pneumonia was studied in a rabbit model. airspace instillation the cytotoxic P. strain PA103 into caused consistent alveolar epithelial injury, progressive bacteremia, and shock. lung noncytotoxic, isogenic mutant (PA103ΔUT), which is defective for production type III secreted toxins, did not cause either systemic inflammatory response or shock, despite potent lung. intravenous injection an increase TNF-α, fact that...
To determine whether liquid and protein clearance from the air spaces lungs of anesthetized unanesthetized dogs is same as in sheep, we quantified these variables at three different time periods (4, 8, 12 h) by instilling heparinized plasma (3 ml/kg) labeled with 125I-albumin into one lower lobe. Protein clearance, measured residual lung homogenate, was slow monoexponential (approximately 1%/h), similar to our previous data for sheep. Lung dogs, however, 50% less than experiments Residual...
Objective: To compare and contrast the process used to implement an early mobility program in ICUs at three different medical centers assess their impact on clinical outcomes critically ill patients. Design: Three ICU mobilization quality improvement projects are summarized utilizing Institute for Healthcare Improvement framework of Plan-Do-Study-Act. Intervention: Each programs required interprofessional team-based approach plan, educate, program. Champions from each profession—nursing,...
Routine preoperative testing is not recommended for patients undergoing cataract surgery, because neither decreases adverse events nor improves outcomes. We sought to assess adherence this guideline, estimate expenditures from potentially unnecessary testing, and identify patient health care system characteristics associated with testing.
Background Translating and scaling healthcare quality improvement (QI) patient safety interventions remains a significant challenge. Context has been identified as major factor in this. QI research have begun to focus on context, with ethnography seen promising methodology for understanding the professional, organisational cultural aspects of context. While is used investigate context variety interventions, challenges inherent effectively importing qualitative its social science...
Pseudomonas aeruginosa is the most frequent Gram-negative pathogen causing nosocomial pneumonia. Four different strains of P. (including three isogenic transposon mutants) were utilized in experiments mice to characterize specific patterns cytokine generation response bacterial products and cytotoxicity. Intratracheal instillation any led up-regulation IL-1beta, IL-6, TNF-alpha mRNA. Instillation cytotoxic (PA103, PA103tox::omega) IL-10 mRNA lungs increased concentrations blood. In contrast,...
Recent studies have found substantial variation in hospital resource use by expired Medicare beneficiaries with chronic illnesses. By analyzing only patients, these cannot identify differences across hospitals health outcomes like mortality. This study examines the association between mortality and at level, when all hospitalized for heart failure are examined.A total of 3999 individuals a principal diagnosis 6 California teaching January 1, 2001, June 30, 2005, were analyzed multivariate...
To determine the lung microvascular pressure profile during hypoxia, we micropunctured subpleural microcirculation of isolated perfused cat lungs. Our procedures involved exsanguinating a cat, then cannulating its pulmonary artery, left atrium, and trachea. Using cat's own blood, lungs at artery atrial pressures 18 9 cm water, respectively, to obtain blood flow 81 +/- 29 ml/(kg body weight x min), which held constant throughout experiment. We stabilized surface with vacuum ring 30-...
We have directly measured lung interstitial fluid pressure at sites of filtration by micropuncturing excised left lower lobes dog lung. blood-perfused each lobe after cannulating its artery, vein, and bronchus to produce a desired amount edema. Then, stop further edema, we air-embolized the lobe. Holding constant airway 5 cmH2O, using beveled glass micropipettes servo-null method. In 31 lobes, divided into 6 groups according severity micropunctured subpleural interstitium in alveolar wall...
We studied anesthetized sheep to determine the relationship between increased permeability pulmonary edema and development mechanism of pleural effusion formation. In 12 with intact, closed thoraces, we time course liquid formation after 0.12 ml/kg i.v. oleic acid. After 1 h, there were no effusions, even though extravascular lung water 50% 6.0 +/- 0.7 g/g dry lung. By 3 h effusions had formed, they reached a maximum at 5 (48.5 16.9 ml/thorax), 8 was additional accumulation (45.5 ml)....