- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Microbial Fuel Cells and Bioremediation
- biodegradable polymer synthesis and properties
- Enzyme Catalysis and Immobilization
- Marine and coastal ecosystems
- Microbial Metabolic Engineering and Bioproduction
National Renewable Energy Laboratory
2019-2023
3-Hydroxybutyrate (3HB) is a product of interest as it precursor to the commercially produced bioplastic polyhydroxybutyrate. It can also serve platform for fine chemicals, medicines, and biofuels, making value-added feedstock. Acetogens non-photosynthetically fix CO2 into acetyl-CoA have been previously engineered convert 3HB. However, acetogen metabolism poorly understood, those engineering efforts had varying levels success. 3HB, using precursor, be synthesized by variety different...
Rising global greenhouse gas emissions and the impacts of resultant climate change necessitate development deployment carbon capture conversion technologies. Amongst myriad bio-based approaches under evaluation, a formate bio-economy has recently been proposed, wherein CO2-derived serves as substrate for concurrent energy delivery to microbial systems. To date, this approach explored in chemolithotrophic heterotrophic organisms via native or engineered formatotrophy. However, utilization...
Ferredoxin5 (FDX5), a minor ferredoxin protein in the alga Chlamydomonas (Chlamydomonas reinhardtii), helps maintain thylakoid membrane integrity dark. Sulfur (S) deprivation has been used to achieve prolonged hydrogen production green algae. Here, we propose that FDX5 is involved algal responses S-deprivation as well Specifically, tested role of both initial aerobic and subsequent anaerobic phases S-deprivation. Under S-deprived conditions, absence causes distinct delay achieving anoxia by...