A5078834398- Neuropeptides and Animal Physiology
- Gastrointestinal motility and disorders
- Receptor Mechanisms and Signaling
- Biochemical Analysis and Sensing Techniques
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Regulation of Appetite and Obesity
- Pancreatic function and diabetes
- Diet and metabolism studies
- Gastroesophageal reflux and treatments
- Olfactory and Sensory Function Studies
- Pharmacological Receptor Mechanisms and Effects
- Pain Mechanisms and Treatments
- Cancer, Stress, Anesthesia, and Immune Response
- Helicobacter pylori-related gastroenterology studies
- Advanced Chemical Sensor Technologies
- Peptidase Inhibition and Analysis
- Pancreatitis Pathology and Treatment
- Neuroscience and Neuropharmacology Research
- Congenital gastrointestinal and neural anomalies
- Diabetes Treatment and Management
- Neuroscience of respiration and sleep
- Neuroendocrine Tumor Research Advances
- Hypothalamic control of reproductive hormones
- Nerve injury and regeneration
- Infant Health and Development
University of California, Los Angeles
2014-2024
UCLA Health
1991-2023
VA Greater Los Angeles Healthcare System
2003-2016
Charité - Universitätsmedizin Berlin
2011
Aarhus University Hospital
2011
Martin-Luther-Krankenhaus
2011
VA West Los Angeles Medical Center
2001-2008
Center for Digestive and Liver Diseases
1993-2008
GTx (United States)
2008
Digestive Care (United States)
2004
Opiate alkaloids are potent analgesics that exert multiple pharmacological effects in the nervous system by activating G protein-coupled receptors. Receptor internalization upon stimulation may be important for desensitization and resensitization, which affect cellular responsiveness to ligands. Here, we investigated agonist-induced of mu opioid receptor (MOR) vivo using guinea pig ileum as a model immunohistochemistry with an affinity-purified antibody C terminus rat MOR. Antibody...
Specific transport proteins package classical neurotransmitters into vesicles so that their release can be regulated by neural activity. Previous studies have suggested a single activity mediates the vesicular of monoamines in adrenal gland, brain, and other tissues such as mast cells platelets. However, molecular cloning has recently identified two transporters for monoamines. Although predicted are closely related sequence, they show range differences physiologic pharmacologic properties....
In view of the importance molecular sensing in function gastrointestinal (GI) tract, we assessed whether signal transduction proteins that mediate taste signaling are expressed cells human gut. Here, demonstrated alpha-subunit taste-specific G protein gustducin (Galpha(gust)) is prominently colon also contain chromogranin A, an established marker endocrine cells. Double-labeling immunofluorescence and staining serial sections Galpha(gust) localized to enteroendocrine L express peptide YY...
Abstract µ‐, δ‐ and κ‐opioid receptors (ORs) mediate the effects of endogenous opioids opiate drugs. Here we report (1) distribution µOR in guinea‐pig human gastrointestinal tract relation to ligands, functionally distinct structures gut δOR κOR; (2) ligand‐induced endocytosis enteric neurones using vitro vivo models. In guinea pig, immunoreactivity is confined mainly myenteric plexus. are most numerous small intestine, followed by stomach proximal colon. immunoreactive fibres dense muscle...
Abstract The distribution of calcitonin‐gene‐related peptide (CGRP) immunoreactivity (IR) was studied in peripheral tissues rats. ganglionic origin, somatosensory nature, and anatomic relations this thin‐axon population were evaluated with particular emphasis on possible nociceptive roles. In animals untreated colchicine, CGRP‐IR is found a vast proportion small‐ medium‐diameter sensory ganglion cells that give rise to numerous thinly myelinated unmyelinated axons display throughout the...
Intestinal perfusion with carbohydrates inhibits gastric emptying via vagal and spinal capsaicin-sensitive afferent pathways. The aim of the present study was to determine role 1) 5-hydroxytryptamine (5-HT) 3 receptors (5-HT R) in mediating glucose-induced inhibition 2) 5-HT R expression afferents innervating duodenum. In awake rats fitted duodenal cannulas, duodenum glucose (50 100 mg) inhibited emptying. mannitol only at highest concentration (990 mosm/kgH 2 O). Pretreatment antagonist...
Abstract The distribution of the neuropeptide calcitonin gene‐related peptide (CGRP) was studied in relation to known subcortical somatosensory pathways and contiguous systems central nervous system (CNS) rats by using peroxidase histochemical methods order relate zones immunoreactivity (IR) cytoarchitecture. CGRP is most ubiquitous found date sensory ganglion cells: principally small medium‐size neurons emitting thin axons inferred be largely nociceptive function on basis peripheral their...
Abstract In the digestive system, substance P is an excitatory transmitter to muscle, a putative neuro‐neuronal transmitter, vasodilator, and mediator in inflammatory processes. Many of biological effects are mediated by high‐affinity interaction with tachykinin receptor neurokinin‐1. The aim present study was identify sites expression this rat stomach intestine immunchistochernistry polyclonal antiserum raised intracellular C‐terminal portion neurokinin‐1 receptor. Neurokinin‐1‐...
Abstract The source, distribution, and morphology of axons displaying calcitonin gene‐related peptide (CGRP) immunoreactivity in the central amygdaloid nucleus adult rat were investigated with immunohistochemical techniques, both alone combination retrograde transport fluorescent tracers. An extremely dense plexus CGRP‐immunoreactive is differentially concentrated within lateral capsular subdivisions nucleus, much lighter concentrations labeled fibers are present rostral part medial...
Changes in the luminal contents of gastrointestinal tract modulate functions, including absorption nutrients, food intake, and protection against harmful substances. The current notion is that mucosal enteroendocrine cells act as primary chemoreceptors by releasing signaling molecules response to changes environment, which turn activate nerve terminals. recent discovery taste receptors G protein subunits alpha-gustducin alpha-transducin, involved gustatory signal transduction, are expressed...
Calcitonin receptor-like receptor (CLR) and activity modifying protein 1 (RAMP1) comprise a for calcitonin gene related peptide (CGRP) intermedin. Although CGRP is widely expressed in the nervous system, less known about localization of CLR RAMP1. To localize these proteins, we raised antibodies to Antibodies specifically interacted with RAMP1 HEK cells coexpressing rat RAMP1, determined by Western blotting immunofluorescence. Fluorescent bound surface CGRP, CLR, internalized into same...
Somatostatin exerts multiple effects by activating distinct G protein-coupled receptors. Here we report the cellular sites of expression somatostatin subtype 2A (sst2A) receptor in rat enteric nervous system using a C-terminus-specific, affinity-purified antiserum and immunohistochemistry. Antibody specificity was confirmed cell surface staining human embryonic kidney 293 cells expressing sst2A receptor, lack 2B abolition preincubating with C-terminus peptide used for immunization,...