A5045040590- Agriculture, Plant Science, Crop Management
- Wheat and Barley Genetics and Pathology
- Plant tissue culture and regeneration
- Plant Disease Resistance and Genetics
- Plant Virus Research Studies
- Plant Reproductive Biology
- Plant Genetic and Mutation Studies
- CRISPR and Genetic Engineering
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Plant nutrient uptake and metabolism
- Transgenic Plants and Applications
- Agricultural economics and policies
- Plant Pathogens and Resistance
- Food composition and properties
- Seed and Plant Biochemistry
- Crop Yield and Soil Fertility
- Mining and Industrial Processes
- Genetics and Plant Breeding
- Genetic Mapping and Diversity in Plants and Animals
- Agricultural Engineering and Mechanization
- Plant pathogens and resistance mechanisms
- Advancements in Materials Engineering
- Berry genetics and cultivation research
- RNA and protein synthesis mechanisms
Plant Breeding and Acclimatization Institute - National Research Institute
2012-2024
Adam Mickiewicz University in Poznań
2018
Max Planck Institute for Biology
2018
Warsaw University of Life Sciences
2018
Institute of Plant Genetics, Polish Academy of Sciences
2018
Polish Academy of Sciences
2018
Stable RNA interference-based technology was used to silence the expression of HvCKX1 gene in barley and TaCKX1 wheat triticale. The silencing cassettes containing fragments these genes sense antisense orientations were cloned into pMCG161 binary vector for Agrobacterium-based transformation. Out five cultivars representing three studied species, transgenic plants obtained from one cultivar Golden Promise, Kontesa, triticale Wanad. Almost 80% 52 regenerated lines Promise exhibited...
Genome editing of monocot plants can be accomplished by using the components CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR associated Cas9) technology specifically optimized for these types plants. Here, we present development RNA-guided Cas9 system simplex and multiplex genome in barley. We developed a set customizable binary vectors sgRNA modules To facilitate design constructs, pBract derived were adapted to Gateway cloning only one restriction enzyme was...
Barley is among four of the most important cereal crops with respect to global production. Increasing barley yields desired levels can be achieved by genetic manipulation cytokinin content. Cytokinins are plant hormones that regulate many developmental processes and have a strong influence on grain yield. Cytokinin homeostasis regulated members several multigene families. CKX genes encode oxidase/dehydrogenase enzyme, which catalyzes irreversible degradation cytokinin. Several recent studies...
Cytokinin oxidase/dehydrogenase proteins (CKX) are encoded by a multigene family of CKX genes with varying number members depending on species. For some the genes, spectacular effects grain production in selected cereals have been observed. Despite fact that partial or full length sequences most HvCKX barley (Hordeum vulgare) already published, cases their specific biological functions not reported. Detailed expression patterns for five different organs/tissues developing plants coupled...
CKX genes encode cytokinin dehydrogenase enzymes (CKX), which metabolize cytokinins in plants and influence developmental processes. The are expressed different tissues organs during development; however, their exact role barley is poorly understood. It has already been proven that RNA interference (RNAi)-based silencing of HvCKX1 decreased the level, especially those showed highest expression, i.e. developing kernels roots, leading to higher plant productivity mass roots [1]. same type RNAi...
The RNAi-mediated silencing of Pina and Pinb, the two genes responsible for grain texture allohexaploid wheat, was induced analysed in wheat cultivars, Kontesa Torka. A characterization non-transgenic plants revealed that Pinb carries a point mutation, designated Pinb-D1c both cultivars. This mutation does not influence transcript abundance or protein content. Two cassettes hpRNA type were constructed used stable transformation via Agrobacterium. In total, 43 transgenic lines representing...
Gene silencing by RNA interference (RNAi) is a particularly important tool in the study of gene function polyploid cereal species for which collections natural or induced mutants are very limited. Previously we have been testing small interfering (siRNA)-based approach wheat and triticale. In this research artificial microRNAs (amiRs) were studied same target genes to compare effectiveness both pathways. amiR cassettes designed silence Puroindoline (Pina) b (Pinb) hardness their orthologues...
Multigene families of CKX genes encode cytokinin oxidase/dehydrogenase proteins (CKX), which regulate content in organs developing plants. It has already been documented that some them play important roles plant productivity. The presented research is the first step comprehensive characterization bread wheat TaCKX gene family with goal to select determining yield-related traits. specificity expression patterns fifteen formerly annotated members was tested different during development. Based...
This paper reports on the successful Agrobacterium-mediated transformation of oat, and some factors influencing this process. In first step experiments, three cultivars, two types explant, combinations strain/vectors, which were successfully used for other cereals tested. Transgenic plants obtained from immature embryos cvs. Bajka, Slawko Akt leaf base explants cv. Bajka after with A. thumefaciens strain LBA4404(pTOK233). The highest rate (12.3%) was Bajka. About 79% selected proved to be...
A new approach to determining the friabilin content of wheat grain was proposed. Electropherograms were taken, and intensity bands compared in analyzed cultivars cv. Chinese Spring. The indicator calculated 17 common cultivars, which differed mostly their crude protein hardness index (HI). basic properties kernels measured each cultivar, correlations between parameters determined. In ranged from around 0.21 0.77. This significantly correlated with kernel length, thickness, mass,...
As an essential regulatory component in plants, microRNAs (miRNAs) have been intensively studied over the past decade. Although hundreds of miRNAs identified and analyzed many important crops model very little is known about function common wheat (Triticum aestivum L.) miRNAs. In this study, we performed computational prediction novel based on BLAST searches expressed sequence tag database. The expression profiles all were for both vegetative reproductive tissues to identify developmentally...
TaCKX gene family members (GFMs) play essential roles in the regulation of cytokinin during wheat development and significantly influence yield-related traits. However, detailed function most them is not known. To characterize role TaCKX2.2 genes we silenced all homoeologous copies both TaCKX2.2.1 TaCKX2.2.2 by RNAi technology observed effect silencing 7 DAP spikes T1 T2 generations. The levels these developmentally regulated were different generations, which variously determined particular...
Secaloindoline a (Sina) and secaloindoline b (Sinb) genes of hexaploid triticale (x Triticosecale Wittmack) are orthologs puroindoline (Pina) (Pinb) in wheat (Triticum aestivum L.). It has already been proven that RNA interference (RNAi)-based silencing Pina Pinb significantly decreased the proteins essentially increased grain hardness (J Exp Bot 62:4025-4036, 2011). The function Sina Sinb was tested by means RNAi compared to wheat. Novel alleles wild-type plants cv. Wanad were identified...
Wheat (Triticum aestivum L.) grain hardness is determined mainly by variations in puroindoline genes (Pina-D1 and Pinb-D1), which are located on the short arm of chromosome 5D. This trait has a direct effect technological properties flour final product quality. The objective study was to analyze mutation frequency both Pin their influence 118 modern bread wheat cultivars breeding lines cultivated Poland, 80 landraces from Poland. PCR products containing gene coding sequences were sequenced...
Long noncoding RNAs (lncRNAs) are a class of RNA molecules with gene regulatory functions in plant development and the stress response. Although number lncRNAs identified plants is rapidly increasing, very little known about their role barley development. In this study, we performed global identification based on 53 RNAseq libraries derived from nine different tissues organs. total, 17,250 10,883 loci were identified, including 8954 novel lncRNAs. Differential expression was observed...
Twardość ziarna pszenicy heksaploidalnej T. aestivum jest cechą determinowaną między innymi przez geny puroindolinowe Pina i Pinb. Geny te kodują białka PINA PINB, które akumulowane są w endospermie na powierzchni ziaren skrobiowych. Pinb występują genomie D oraz genomach pszenic diploidalnych, natomiast pszenica tetraploidalna o AABB nie posiada tych genów. Ortologi genów puroindolinowych również u innych gatunków zbóż wykazują ponad 90% podobieństwo z sekwencją kodującą Pin pszenicy. U...
in Poznań where he completed the studies biology and started to teach 1956.He graduated 1957 with a master's degree botany working on root tissue culture of carrot.Then continued male sterility carrot which also earned him his PhD 1962.He became world-known scientist experimental embryology thanks works vitro pollination ovules embryo rescue distant crosses