A5033423810- Pancreatic function and diabetes
- Diabetes Management and Research
- Retinal and Optic Conditions
- Retinal Diseases and Treatments
- Retinal Imaging and Analysis
- Ocular Diseases and Behçet’s Syndrome
- Glaucoma and retinal disorders
- Cytomegalovirus and herpesvirus research
- Diabetes and associated disorders
- Cannabis and Cannabinoid Research
- Cardiovascular Health and Disease Prevention
- RNA Interference and Gene Delivery
- Metabolism, Diabetes, and Cancer
- Intraocular Surgery and Lenses
- Blood Pressure and Hypertension Studies
- Intracranial Aneurysms: Treatment and Complications
- Sinusitis and nasal conditions
- Digital Imaging for Blood Diseases
- Mosquito-borne diseases and control
- Apelin-related biomedical research
- Protein purification and stability
- Hemoglobinopathies and Related Disorders
- Erythrocyte Function and Pathophysiology
- Ocular Infections and Treatments
- Electrolyte and hormonal disorders
City of Hope
2014-2023
Hospital de Galdakao
2019
Beckman Research Institute
2014-2018
Hospital Universitari Germans Trias i Pujol
2016
We evaluated three commercially available enzymes for pancreatic digestion by comparing key parameters during the islet isolation process, as well quality post-isolation.Retrospectively compared and analyzed isolations from pancreata using different enzyme groups: Liberase HI (n=63), Collagenase NB1/Neutral Protease (NP) (n=43), Mammalian Tissue Free Collagenase/Thermolysin (MTF C/T) (n=115). A standardized purification method was used. Islet assessment carried out count, viability, in vitro...
The aim of this study was to investigate the effects elevated donor HbA1c levels (type 2 diabetes, T2D) on islet yield and functionality postisolation. In retrospective analysis, donors for isolations were classified into two groups: T2D group (HbA1c ≥ 6.5%, n = 18) normal < 308). Optimum pancreas digestion time (switch time) significantly higher in compared (13.7 ± 1.2 vs. 11.7 0.1 min, respectively, p 0.005). Islet yields lower control (T2D control): equivalent (IEQ)/g (prepurification...
Organs from hypernatremia (elevated Na+) donors when used for transplantation have had dismal outcomes. However, islet isolation hypernatremic both and research applications has not yet been investigated. A retrospective analysis of in vivo vitro function studies was performed on islets isolated (serum sodium levels≥160 meq/l) normal control levels≤155 donors. Twelve isolations 32 53 222 were randomly transplanted into diabetic NOD Scid mice. Sodium levels upon pancreas procurement...
Islet transplantation has made major progress to treat patients with type 1 diabetes. mass and quality are critically important ensure successful transplantation. Currently, islet status is evaluated using insulin secretion, oxygen consumption rate, or adenosine triphosphate (ATP) measurement. These parameters independently do not effectively predict post-transplant. Therefore, assessing human pancreatic islets by encompassing ATP, DNA, insulin, protein content from a single tissue sample...
Abstract A novel peptide substrate (A G P L G) was developed for quantifying the activities of bacterial enzymes using a highly sensitive Fluorescence Resonance Energy Transfer (FRET) based assay. The cleaved by collagenase class I, II, Liberase MTF C/T, NB1, and thermolysin/neutral protease, which significantly enhanced in presence CaCl 2 . However, these were decreased ZnSO 4 or ZnCl Collagenase protease share similar cleavage sites, ↓ G. NB1 cleaves at L, addition to enzyme activity is pH...
Transplanting pancreatic islets into recipients must be safe and effective to treat Type 1 diabetes. Islet quality quantity are important, however, the final product also free from microbial contamination low endotoxin levels.This study explored a method eliminate in manufacturing for transplantation. A simple (single antibiotic, n=164) refined (triple antimicrobial agents, n=279) pancreas decontaminating methods were used test their effects on reducing rates islet product. total of 443...
Selection of enzymes for optimal pancreas digestion is essential successful human islet isolations. The aim this study was to evaluate the efficacy and outcome using Collagenase Gold plus BP protease (VitaCyte) (n = 8) by comparing it two commercially available enzymes, Liberase MTF C/T (Roche) 48) NB1/NP (Serva) 15). isolation outcomes were assessed counting, viability, glucose-stimulated oxygen consumption rate (OCR), graft-rate following transplantation in diabetic NOD scid mice. donor...
Introduction. Islet transplantation (IT) restores insulin production in individuals with severe type 1 diabetes (T1D). However, after isolation, human islets rapidly loose function isolation and must be transplanted within about two to three days for best outcomes. This limits access standard IT combined kidney transplantation. Current methods of cultivating isolated fail appreciate the role extracellular matrix islet health do not permit long-term culture. In this study, were cultured a 3D...