A5032504909- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Bacteriophages and microbial interactions
- Biosensors and Analytical Detection
- Modular Robots and Swarm Intelligence
- RNA and protein synthesis mechanisms
- bioluminescence and chemiluminescence research
- Energy Harvesting in Wireless Networks
Aarhus University
2016-2018
Fluorescent RNA aptamers are useful as markers for tracking molecules inside cells and creating biosensor devices. Förster resonance energy transfer (FRET) based on fluorescent proteins has been used to detect conformational changes, however, such FRET devices have not yet produced using aptamers. Here we develop an aptamer-based (apta-FRET) system single-stranded origami scaffolds. To obtain FRET, the Spinach Mango placed in close proximity scaffolds a new fluorophore is synthesized...
Biosensors play increasingly important roles in many fields, from clinical diagnosis to environmental monitoring, and there is a growing need for cheap simple analytical devices. DNA nanotechnology provides methods the creation of sophisticated biosensors, however developed DNA-based sensors are limited by cumbersome time-consuming readouts involving advanced experimental techniques. Here we describe design, construction, characterization an optical origami nanobiosensor device exploiting...
The fields of DNA and RNA nanotechnology have established nucleic acids as valuable building blocks for functional nanodevices with applications in nanomedicine. Here, a simple method designing assembling 3D scaffolded origami wireframe structure intrinsic functioning small interfering RNAs (siRNAs) embedded is introduced. Uniquely, the uses an mRNA fragment scaffold strand, which folded by sequence-complementarity nine shorter synthetic strands. High-yield production intended verified...