A5023128027- Metabolomics and Mass Spectrometry Studies
- Pharmacogenetics and Drug Metabolism
- Estrogen and related hormone effects
- Organometallic Complex Synthesis and Catalysis
- Adenosine and Purinergic Signaling
- Cytokine Signaling Pathways and Interactions
- Eicosanoids and Hypertension Pharmacology
- Genomics, phytochemicals, and oxidative stress
- Drug Transport and Resistance Mechanisms
- NF-κB Signaling Pathways
- Click Chemistry and Applications
- Catalytic Cross-Coupling Reactions
- Hormonal Regulation and Hypertension
- Genomics and Chromatin Dynamics
- Biochemical effects in animals
- Liver Disease Diagnosis and Treatment
- Biochemical Acid Research Studies
- Antioxidant Activity and Oxidative Stress
- Biochemical and Molecular Research
- Boron Compounds in Chemistry
- Redox biology and oxidative stress
- HIV/AIDS drug development and treatment
- Fatty Acid Research and Health
- Retinoids in leukemia and cellular processes
- Cyclopropane Reaction Mechanisms
National Institute of Diabetes and Digestive and Kidney Diseases
2009-2024
National Institutes of Health
2009-2024
Taegu Science University
2013
National Cancer Institute
2012
Center for Cancer Research
2012
University of Bern
2012
University of California, San Francisco
2002-2007
University of California, Berkeley
1996-1998
Lawrence Berkeley National Laboratory
1996-1998
Members of the mammalian p160 family, such as GRIP1, are known glucocorticoid receptor (GR) coactivators; at certain response elements (GREs), however, GRIP1 acts a GR corepressor. We characterized functional interactions and in repression complex where tethers to DNA-bound activator protein-1 (AP-1), human collagenase-3 gene, tested whether identified were similar or different other elements. At AP-1 tethering GRE, we mapped corepressor activity domain distinct from two activation domains;...
To investigate the determinants of promoter-specific gene regulation by glucocorticoid receptor (GR), we compared composition and function regulatory complexes at two NFκB-responsive genes that are differentially regulated GR. Transcription IL-8 IκBα is stimulated TNFα in A549 cells, but GR selectively represses mRNA synthesis inhibiting Ser2 phosphorylation RNA polymerase II (pol II) C-terminal domain (CTD). The proximal κB elements these differ sequence a single base pair, both recruited...
Radiation metabolomics has aided in the identification of a number biomarkers cells and mice by ultra-performance liquid chromatography-coupled time-of-flight mass spectrometry (UPLC-ESI-QTOFMS) rats gas (GCMS). These markers have been shown to be both dose- time-dependent. Here UPLC-ESI-QTOFMS was used analyze rat urine samples taken from 12 over 7 days; they were either sham-irradiated or γ-irradiated with 3 Gy after 4 days metabolic cage acclimatization. Using multivariate data analysis,...
Mass spectrometry-based metabolomics has previously demonstrated utility for identifying biomarkers of ionizing radiation exposure in cellular, mouse and rat vivo models. To provide a valuable link from small laboratory rodents to humans, γ-radiation-induced urinary were investigated using nonhuman primate total-body-irradiation model. approaches applied determine whether could be identified, as well the discovered rodent γ radiation. Ultra-performance liquid chromatography-electrospray...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTSynthesis of Fischer Carbene Complexes Iridium by C−H Bond Activation Methyl and Cyclic Ethers: Evidence for Reversible α-Hydrogen MigrationHans F. Luecke, Bruce A. Arndtsen, Peter Burger, Robert G. BergmanView Author Information Chemical Sciences Division, Lawrence Berkeley Laboratory Department Chemistry University California, Berkeley, California 94720 Cite this: J. Am. Chem. Soc. 1996, 118, 10, 2517–2518Publication Date (Web):March 13,...
Schistosomiasis is a chronic parasitic disease affecting hundreds of millions individuals worldwide. Current treatment depends on single agent, praziquantel, raising concerns emergence resistant parasites. Here, we continue our explorations an oxadiazole-2-oxide class compounds recently identified as inhibitors thioredoxin glutathione reductase (TGR), selenocysteine-containing flavoenzyme required by the parasite to maintain proper cellular redox balance. Through systematic evaluation core...
Replication foci are generated by many viruses to concentrate and localize viral DNA synthesis specific regions of the cell. Expression HPV16 E1 E2 replication proteins in keratinocytes results nuclear that recruit associated with host damage response. We show Brd4 protein localizes these is essential for their formation. However, when begin amplifying DNA, displaced from cellular factors homologous recombination recruited. Differentiated HPV-infected form similar contain DNA. compare...
Pregnane X receptor (PXR) is an important nuclear xenosensor that regulates the expression of metabolic enzymes and transporters involved in metabolism xenobiotics endobiotics. In this study, ultra-performance liquid chromatography (UPLC) coupled with electrospray time-of-flight mass spectrometry (TOFMS), revealed altered urinary metabolomes both Pxr-null wild-type mice treated mouse PXR activator pregnenolone 16alpha-carbonitrile (PCN). Multivariate data analysis PCN significantly...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTSynthesis and C−H Activation Reactions of Cyclometalated Complexes Ir(III): Cp*(PMe3)Ir(CH3)+ Does Not Undergo Intermolecular in Solution via a IntermediateHans F. Luecke Robert G. BergmanView Author Information Chemical Sciences Division, Lawrence Berkeley Laboratory the Department Chemistry University California, Berkeley, California 94720 Cite this: J. Am. Chem. Soc. 1997, 119, 47, 11538–11539Publication Date (Web):November 26,...
Ligand binding to the glucocorticoid receptor (GR) results in response elements (GREs) and formation of transcriptional regulatory complexes. Equally important, these complexes are continuously disassembled, with active processes driving GR off GREs. We found that co-chaperone p23-dependent disruption GR-driven transcription depended on ligand domain (LBD). Next, we examined importance LBD dissociation GR-GRE living cells. showed fluorescence recovery after photobleaching studies from GREs...
Farnesoid X receptor (FXR) is a nuclear that regulates genes involved in synthesis, metabolism, and transport of bile acids thus plays major role maintaining acid homeostasis. In this study, metabolomic responses were investigated urine wild-type Fxr-null mice fed cholic acid, an FXR ligand, using ultra-performance liquid chromatography (UPLC) coupled with electrospray time-of-flight mass spectrometry (TOFMS). Multivariate data analysis between on diet revealed the most increased ions...
Transforming growth factor-β (TGFβ) is activated as a result of liver injury, such cholestasis. However, its influence on endogenous metabolism not known. This study demonstrated that TGFβ regulates hepatic phospholipid and bile acid homeostasis through MAD homolog 3 (SMAD3) activation revealed by lithocholic acid-induced experimental intrahepatic Lithocholic (LCA) induced expression TGFB1 the receptors TGFBR1 TGFBR2 in liver. In addition, immunohistochemistry higher around portal vein after...
The pregnane X receptor (PXR) has been postulated to play a role in the metabolism of <i>α</i>-tocopherol owing up-regulation hepatic cytochrome P450 (P450) 3A human cell lines and murine models after treatment. However, vivo studies confirming PXR humans presents significant difficulties not performed. <i>PXR</i>-humanized (h<i>PXR</i>), wild-type, <i>Pxr</i>-null mouse were used determine whether is influenced by species-specific differences function vivo. No difference concentration major...
Alterations in mitochondrial function are the linchpin numerous disease states including development of chemotherapy-induced neuropathic pain (CIPN), a major dose-limiting toxicity widely used chemotherapeutic cytotoxins. In CIPN, dysfunction is characterized by deficits bioenergetics (e.g., decreased ATP production) that thought to drive degeneration peripheral nerve sensory axon terminal arbors skin (the intraepidermal fibers; IENFs) and induce abnormal spontaneous discharge axons....
Cytochrome P450 2E1 (CYP2E1) is a key enzyme in the metabolic activation of many low molecular weight toxicants and also an important contributor to oxidative stress. A noninvasive method monitor CYP2E1 activity vivo would be great value for studying role chemical-induced toxicities stress-related diseases. In this study, mass spectrometry–based metabolomic approach was used identify metabolite biomarker through comparing urine metabolomes wild-type (WT), Cyp2e1-null, CYP2E1-humanized mice....
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTSynthesis, Structural Characterization, and Chemistry of a Monomeric Cationic Iridium Carbyne ComplexHans F. Luecke Robert G. BergmanView Author Information Department Chemistry, University California Berkeley, California, the Chemical Sciences Division Lawrence Berkeley National Laboratory, 94720 Cite this: J. Am. Chem. Soc. 1998, 120, 42, 11008–11009Publication Date (Web):October 9, 1998Publication History Received27 July 1998Published...
A systematically designed panel of biorthogonal pro-metabolites was synthesized and evaluated as agents for tracing cellular short chain fatty acylation.
For HIV to become infectious, any new virion produced from an infected cell must undergo a maturation process that involves the assembly of viral polyproteins Gag and Gag–Pol at membrane surface. The self-assembly these proteins drives formation particle as well activation protease, which is needed cleave so final core structure virus will properly form. Molecules interfere with prevent virions infecting additional cells. In this manuscript, we characterize unique mechanism by...
<TEX>${\alpha}$</TEX>-Muricholic acid was synthesized through 9 steps from chenodeoxycholic with 26% overall yield. Hyocholic 8 the same starting material 63% Taurine conjugates of <TEX>${\alpha}$</TEX>-muricholic and hyocholic were also prepared via their pentafluorophenyl ester.