A5077811310- Plant-Microbe Interactions and Immunity
- Plant Stress Responses and Tolerance
- Advanced Proteomics Techniques and Applications
- Insect-Plant Interactions and Control
- Photosynthetic Processes and Mechanisms
- Insect and Pesticide Research
- Plant Parasitism and Resistance
- Insect Resistance and Genetics
- Plant responses to elevated CO2
- Plant Genetic and Mutation Studies
- Soybean genetics and cultivation
- Genomics and Phylogenetic Studies
- GABA and Rice Research
- Metabolomics and Mass Spectrometry Studies
- Plant tissue culture and regeneration
- Plant Pathogenic Bacteria Studies
- Fungal and yeast genetics research
- Plant Gene Expression Analysis
- Plant nutrient uptake and metabolism
- Mass Spectrometry Techniques and Applications
- Legume Nitrogen Fixing Symbiosis
- Neuropeptides and Animal Physiology
- Plant responses to water stress
- Neurogenesis and neuroplasticity mechanisms
- Biofuel production and bioconversion
University of Tsukuba
2015-2024
Tsukuba International University
2015-2024
Nepal Academy of Science and Technology
2015-2022
Turība University
2022
Hoshi University
2015-2021
Ebara (Japan)
2015-2021
Showa University
2010-2016
Toho University
2010-2015
Indiana University School of Medicine
2012-2015
International Life Sciences Institute India
2013-2014
MALAT‐1, a long non‐coding RNA, is associated with metastasis, but its role in the metastatic process remains unknown. Here, we show that short‐interfering RNA‐mediated MALAT‐1 silencing impaired vitro cell motility of lung cancer cells and influenced expression numerous genes. In these genes, knockdown any one CTHRC1 , CCT4 HMMR or ROD1 clearly inhibited migration. cells, pre‐mRNA levels were decreased some not all Thus, our findings suggest novel class RNA promotes through transcriptional...
MALAT-1 noncoding RNA is localized to nuclear speckles despite its mRNA-like characteristics. Here, we report the identification of several key factors that promote localization and also provide evidence involved in regulation gene expression. Heterokaryon assays revealed does not shuttle between nucleus cytoplasm. RNAi-mediated repression speckle proteins, RNPS1, SRm160, or IBP160, which are well-known mRNA processing factors, resulted diffusion nucleoplasm. We demonstrated contains two...
The free radicals along with several reactive oxygen species (ROS) and nitrogen (RNS) are known to play a dual role in the biological living system which carries substantial importance terms of signal networking plants. production these active molecules different cellular compartments eventually led oxidative damage. However recent discoveries have evidenced its crucial roles as signaling molecules, activating stress responses against environmental challenges. As can be seen, organelles...
Invention of polymerase chain reaction (PCR) technology by Kary Mullis in 1984 gave birth to real-time PCR. Real-time PCR - detection and expression analysis gene(s) has revolutionized the 21st century biological science due its tremendous application quantitative genotyping, genetic variation inter intra organisms, early diagnosis disease, forensic, name a few. We comprehensively review various aspects PCR, including technological refinement all scientific fields ranging from medical...
Employing classical two-dimensional electrophoresis (2-DE), amino acid sequencing and immunoblot analysis, we examine for the first time effect of ozone, a highly notorious environmental pollutant, on rice seedling proteins. Drastic visible necrotic damage to leaf by ozone consequent increase in ascorbate peroxidase protein(s) was accompanied rapid changes 2-DE protein profiles, over controls. Out total 56 proteins investigated, which were reproducible repeated experiments, 52 spots visually...
Employing high-resolution two-dimensional electrophoresis (2-DE), we studied changes in the rice leaf protein patterns, response to applied heavy and alkaline metals, important environmental pollutants our surroundings. Drastic 2-DE patterns after treatment with copper, cadmium, mercury, over control were found, including morphology of segments. Changes major photosynthetic protein, ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO, both suppression fragmentation), induction proteins...
By using an in vivo hydroponic rice seedling culture system, we investigated the physiological and biochemical responses of a model japonica cultivar Nipponbare to salt stress proteomics classical methods. Yoshida's nutrient solution (YS) was used grow seedlings. YS-grown 18-day-old seedlings manifested highly stable reproducible symptoms, prominently wilting browning 3rd leaf, reduced photosynthetic activity, inhibition overall growth, failure develop new (5th) when subjected by...
Ozone (O3), a serious air pollutant, is known to significantly reduce photosynthesis, growth, and yield cause foliar injury senescence. Here, integrated transcriptomics, proteomics, metabolomics approaches were applied investigate the molecular responses of O3 in leaves 2-week-old rice (cv. Nipponbare) seedlings exposed 0.2 ppm for period 24 h. On basis morphological alteration O3-exposed leaves, transcript profiling genes was performed 1, 12, h using DNA microarray chip. A total 1535...
Peanut genotypes from the US mini-core collection were analysed for changes in leaf proteins during reproductive stage growth under water-deficit stress. One- and two-dimensional gel electrophoresis (1- 2-DGE) was performed on soluble protein extracts of selected tolerant susceptible genotypes. A total 102 bands/spots by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) quadrupole time-of-flight tandem (Q-TOF MS/MS) analysis. Forty-nine non-redundant...
Our six-week treadmill running training (forced exercise) model has revealed that mild exercise (ME) with an intensity below the lactate threshold (LT) is sufficient to enhance spatial memory, while intense (IE) above LT negates such benefits. To help understand unrevealed neuronal and signaling/molecular mechanisms of intensity-dependent cognitive change, in this rat model, we here investigated plasma corticosterone concentration as a marker stress, adult hippocampal neurogenesis (AHN)...