A5023513323- Iron Metabolism and Disorders
- Trace Elements in Health
- Bacteriophages and microbial interactions
- Supramolecular Self-Assembly in Materials
- Proteins in Food Systems
- Advanced biosensing and bioanalysis techniques
- Antioxidant Activity and Oxidative Stress
- RNA and protein synthesis mechanisms
- Plant Micronutrient Interactions and Effects
- Heavy Metal Exposure and Toxicity
- Phytase and its Applications
- Advanced Nanomaterials in Catalysis
- Advanced Photocatalysis Techniques
- Aquaculture Nutrition and Growth
- Porphyrin Metabolism and Disorders
- Wound Healing and Treatments
- Enzyme Structure and Function
- Muscle metabolism and nutrition
- Invertebrate Immune Response Mechanisms
- Gold and Silver Nanoparticles Synthesis and Applications
- Nanocluster Synthesis and Applications
- Electrospun Nanofibers in Biomedical Applications
- Neurobiology and Insect Physiology Research
- Protein Structure and Dynamics
- Hemoglobinopathies and Related Disorders
China Agricultural University
2016-2025
Chongqing Medical University
2024
Children's Hospital of Chongqing Medical University
2024
Kyoto University
2016-2018
China University of Petroleum, Beijing
2015
Dalian Institute of Chemical Physics
1993
Chinese Academy of Sciences
1993
Owing to diverse functionalities and metal binding abilities, proteins have been proven be promising ligands in the synthesis of gold nanoclusters (Au NCs). In this work, we explored β-lactoglobulin (β-Lg), a protein byproduct generated during cheese processing, as biotemplate for fabrication Au NCs by facile green method first time. The as-prepared are water soluble highly fluorescent exhibit high sensitivity selectivity Hg2+ detection aqueous solution. Interestingly, found that...
It remains challenging to fabricate highly stretchable and adhesive hydrogel dressings for wound healing using simple, safe, green methods. Herein, inspired by the main components of snail mucus, a fully physical double-network (DN) dressing composed fish gelatin (FGel) glycyrrhizic acid (GL) was fabricated, in which FGel provided protein scaffold mimic mucus proteins, while GL mimicked adhesion bioactivity because its abundant carboxyl hydroxyl groups intrinsic immunomodulatory activity. As...
Aromatic–aromatic interactions between natural aromatic amino acids Phe, Tyr, and Trp play crucial roles in protein–protein recognition protein folding. However, the function of such preparation different dimensional, ordered superstructures has not been recognized. Herein, by a combination directionality symmetry axes building blocks strength aromatic–aromatic coming from group acid residues, we built an engineering strategy to construct superlattices. Based on this strategy, substitution...
Ferritins are ubiquitous iron storage proteins where Fe(II) sequestration prevents not only its spontaneous oxidation to Fe(III) but also production of toxic free radicals. Recently, scientists have subverted these nature functions and used ferritin cage structures nanometer dimensions for encapsulation guest molecules such as anti‐cancer drugs or bioactive nutrients based on pH induced disassembly reassembly property. However, prior this study, nanocage was required disassemble under harsh...
Rendering the geometry of protein-based assemblies controllable remains challenging. Protein shell-like nanocages represent particularly interesting targets for designed assembly. Here, we introduce an engineering strategy—key subunit interface redesign (KSIR)—that alters a natural subunit–subunit by selective deletion small number "silent" amino acid residues (no participation in interfacial interactions) into one that triggers generation non-native protein cage. We have applied KSIR to...
Abstract Constructing different protein nanostructures with high-order discrete architectures by using one single building block remains a challenge. Here, we present simple, effective disulfide-mediated approach to prepare set of nanocages geometries from block. By genetically deleting an inherent intra-subunit disulfide bond, can render the conversion 8-mer bowl-like architecture (NF-8) into 24-mer ferritin-like nanocage in solution, while selective insertion inter-subunit bond NF-8...
Summary Phytoproteins have been widely implemented in the food industry benefiting from their good functionalities, vast reserves and relatively low cost. However, phytoproteins frequently experience poor solubility, which limits applications. In our study, solubility of soy protein isolate (SPI) treated with pH‐shifting increased over 1.8 times; however, transglutaminase‐induced gel properties were sacrificed. To solve this problem, walnut (WP) was used to co‐assemble SPI during treatment....
Protein assemblies with high symmetry are widely distributed in nature. Most efforts so far have focused on repurposing these protein assemblies, a strategy that is ultimately limited by the structures available. To overcome this limitation, methods for fabricating novel self-assembling proteins received intensive interest. Herein, reengineering key subunit interfaces of native 24-mer cage octahedral through amino acid residues insertion, we fabricated 16-mer lenticular nanocage whose...
Ferritin, a ubiquitous iron storage protein, has crucial role in innate immunity arthropods, which have no adaptive immune system. Arthropods are thought to two types of ferritin molecules: the secreted type and cytosolic type. Here, we present first crystal structure from crustacean, kuruma prawn (Marsupenaeus japonicus), at 1.16 Å resolution. This shrimp (MjFer) is type, its shows well-conserved fold composed 4-helix bundle that assembles into cage-like 24-mer. The MjFer was more similar...