Glycyrrhiza uralensis is a perennial medicinal plant. It's generally cultivated for three years, and should avoid long-term continuous cultivation. However, unreasonable crop rotation extensive fertilization are common in G. cultivation, which leads to the imbalance of soil microflora structure, obstacle cropping becoming increasingly serious. Some microbial fertilizers such as Bacillus amyloliquefaciens, subtilis, complex agent have advantage regulating community structure improving...

Introduction Codonopsis pilosula is widely sought-after in China as a substitute for the more expensive ginseng. Continuous cropping of C. supports vibrant health-supplement industry but requires significant inputs fertilizers which increase production costs and degrade environment. Methods Here, three environmentally-friendly natural fertilizers, including biochar, bacterial fertilizer, vermicompost, were used at different concentrations (undiluted, diluted 10 times, 50 times) to determine...

Water-soluble constituents in the root exudates of C. pilosula exhibit both allelopathic and auto-toxic properties, which substantially impede its growth. To mitigate constraints associated with continuous cropping pilosula, this study examined impact aqueous extracts from at various developmental stages on seed germination seedling Additionally, we isolated characterized significant allelochemicals. Our findings revealed that inhibitory effects germination, growth, activity antioxidant...

The chemical components and medicinal properties of different parts Angelica sinensis are often used as medicine after being divided into the head, body tail sinensis. In this study, in periods were analyzed by GC-MS for first time, differences accumulation rules obtained. This study demonstrated that composition mainly reflected types relative contents compounds. found number compounds each period change same compound also different. was least April, largest October. content ligustilide...

Abstract Background In recent years, global climate change in tandem with increased human activity has resulted habitat degradation or the migration of rare medicinal plants, potentially impacting quality herbs. Astragalus membranaceus var. mongholicus is a valuable bulk material Northwest China. As demand for this herb continues to increase both domestic and international markets, ensuring sustainable development high-quality Astragali Radix important. study, maximum entropy (Maxent) model...

In this study, a quantitative 1 H NMR method ( H-qNMR) for determining the contents of imperatorin, byakangelicin, and oxypeucedanin in A. dahurica traditional Chinese medicine (TCM) has been established. Dried plant material was extracted exhaustively with methanol by an ultrasonication-assisted extraction method. The H-qNMR measurements were performed on 600 -MHz spectrometer hydroquinone as internal standard reference deuterated dimethyl sulfoxide (DMSO-d 6 ) solvent. Quantification...

Abstract Objective The goal of this work was to establish a method identify and quantify the main active components in Angelicae Pubescentis Radix (APR) quickly, simply, accurately. This paper reports novel which can determine osthol, isoimperatorin, columbianadin using 1H-qNMR simultaneously quantitatively. Methods In comprehensive consideration resolution target signals solubility materials, dimethyl sulfoxide-d6 (DMSO-d6) selected as an optimal solvent pyrazine used internal standard...

The goal of this work was to establish a method identify and quantify the primary active components in Angelicae pubescentis Radix quickly, simply, accurately. This article first reported high-performance liquid chromatography (HPLC) that simultaneously determined quantified osthol, isoimperatorin, columbianadin using quantitative analysis multi-components by single marker (QAMS) which uses internal reference substances relative correction factors perform synchronous determination...

A HPLC method has been developed for simultaneously detecting chlorogenic acid, ferulic senkyunolide I, H, coniferyl ferulate, A, ligustilide, and levistolide in Angelicae Sinensis Radix through quantitative analysis of multicomponents by single-marker (QAMS) with acid as internal standard substance. The relative correction factors each component have good reproducibility under different chromatography conditions. In addition, no significant difference results was found between external...

The content of active components in traditional Chinese medicine is relatively small, and it difficult to detect some trace with modern analytical instruments, so good pretreatment extraction are very important the experiment. Graphene was introduced by a dispersive liquid–liquid microextraction method based on solidification floating organic drop (DLLME-SFO) graphene/1-dodecyl alcohol used as extractant, this method, combined quantitative proton nuclear magnetic resonance spectroscopy...

Abstract Background As known to us, HPLC method was often used determine the contents of Angelicae sinesis Radix. In view shortcomings method, qNMR has prominent advantages in determining bioactive components quantitative and qualitative analysis Objective this study, a quick, simple, accurate established ferulic acid, coniferyl ferulate, ligustilide Method Using dimethyl sulfoxide-d6(DMSO-d6) as test solvent pyrazine internal standard substance, 1H-qNMR measurement performed on 600 MHz...

Abstract Aims Water-soluble components in root exudates of C. pilosula are auto-toxic and allelopathic, severely limiting its growth. To effectively alleviate limitations to continuous cropping . Methods seedlings were cultured by hydroponics aqueous leachate collected. The growth indexs enzyme activity measured under the treatment extracts exudates. Gas chromatography-mass spectrometry (GC-MS) analysis was used qualitatively identify chemical constituents an solution secretion at a...

To investigate the soil-specific metabolites of Codonopsis pilosula under different stubble management practices, this study analyzed differentially abundant in rhizosphere soils rotational (DS) and continuous (LS) cropping systems via liquid chromatography–tandem mass spectrometry (LC–MS/MS)-based metabolomic approaches. The results revealed that 66 metabolites, including amino acids their derivatives, nucleic acids, alcohols, organic amines, fatty purines, sugars, were significantly (p...