A5101428554- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- Acute Lymphoblastic Leukemia research
- Cancer Immunotherapy and Biomarkers
- Cancer Research and Treatments
- Telomeres, Telomerase, and Senescence
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- 3D Printing in Biomedical Research
- Immunotherapy and Immune Responses
- Bone Tissue Engineering Materials
- Renal cell carcinoma treatment
- Cancer Genomics and Diagnostics
- Force Microscopy Techniques and Applications
- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Drug Transport and Resistance Mechanisms
- Dermatologic Treatments and Research
- Lung Cancer Diagnosis and Treatment
- Cardiovascular Effects of Exercise
- Bone fractures and treatments
- Facial Rejuvenation and Surgery Techniques
- Peptidase Inhibition and Analysis
- Gene expression and cancer classification
- CAR-T cell therapy research
University of Cambridge
2022-2024
Medical Research Council
2023
Wellcome/MRC Cambridge Stem Cell Institute
2023
University of Birmingham
2020
Universitat Politècnica de València
2018
Software (Spain)
2018
Universitat de Barcelona
2012-2016
The crucial role of tumor-associated fibroblasts (TAF) in cancer progression is now clear non-small cell lung (NSCLC). However, therapies against TAFs are limited due to a lack understanding the subtype-specific mechanisms underlying their accumulation. Here, mechanical (i.e., matrix rigidity) and soluble mitogenic cues that drive accumulation from major NSCLC subtypes: adenocarcinoma (ADC) squamous carcinoma (SCC) were dissected. Fibroblasts cultured on substrata engineered exhibit normal-...
Cells from lung and other tissues are subjected to forces of opposing directions that largely transmitted through integrin-mediated adhesions. How cells respond force bidirectionality remains ill defined. To address this question, we nanofabricated flat-ended cylindrical Atomic Force Microscopy (AFM) tips with ∼1 µm2 cross-section area. Tips were uncoated or coated either integrin-specific (RGD) non-specific (RGE/BSA) molecules, brought into contact epithelial fibroblasts for 30 s form focal...
Clearance of intracellular infections caused by Salmonella Typhimurium (STm) requires IFN-γ and the Th1-associated transcription factor T-bet. Nevertheless, whereas IFN-γ-/- mice succumb rapidly to STm infections, T-bet-/- do not. In this study, we assess anatomy immune responses relationship with bacterial localization in spleens livers STm-infected mice. mice, there is deficient granuloma formation inducible NO synthase (iNOS) induction, increased dissemination bacteria throughout organs,...
Abstract B-cell acute lymphoblastic leukemia (B-ALL) is an aggressive hematological malignancy of B lineage progenitors. It remains a leading cause death in childhood, while outcomes adults are dismal. There therefore need to better understand drivers high-risk B-ALL and develop novel therapeutic approaches targeting these challenging patient cohorts. Loss-of-function mutations affecting CREBBP recurrent second-hit across multiple genetic subtypes associated with adverse features, including...
Abstract Leucine-rich repeat-containing G-protein receptor 5 (LGR5) has been characterised as a stem cell and cancer marker. Previous analyses of LGR5 transcript levels indicate high level expression discriminates malignancies such colorectal (CRC) pre-B acute lymphoblastic leukaemia (pre-B ALL) from healthy tissues suggesting protein may provide molecular handle for prognosis treatment. We have developed highly specific, affinity antibodies to the extracellular domain human (α-LGR5) that...
Abstract The mechanisms underlying the abundance of tumor associated fibrobasts (TAFs) in non-small cell lung cancer (NSCLC) remain poorly understood. To address this gap knowedge, we examined relative contribution mechanical (i.e. matrix rigidity) and soluble biochemical serum) cues accumulation primary TAFs from either adenocarcinoma (ADC) or squamous carcinoma (SQC) NSCLC patients. For purpose, fibroblasts were cultured on collagen-I coated gels engineered to exhibit normal- tumor-like...
Abstract Breathing demands our lungs to be soft and elastic. In contrast, lung tumors exhibit an abnormal tissue hardening concomitantly with altered architecture function, which brings the mechanical microenvironment of tumor closer that muscle or bone. This has been associated abundant reactive stroma rich in activated fibroblasts (also referred as TAFs) is a hallmark non-small cell cancer (NSCLC). However, it remains unknown whether alone sufficient drive abundance NSCLC. To address this...
Abstract B-cell acute lymphoblastic leukemia (B-ALL) is a leading cause of death in childhood and outcomes adults remain dismal. There therefore an urgent clinical need for therapies that target the highest risk cases. Mutations histone acetyltransferase CREBBP associate with high-risk features B-ALL have been implicated chemoresistance. We performed targeted drug screen isogenic human cell lines, identifying number actionable small molecules specifically -mutated B-ALL. The most potent was...
Abstract Although permanent cell cycle arrest through senescence has been previously regarded as a protective mechanism against tumor growth, there is growing evidence that the abnormal abundance of senescent cells observed in stroma some types cancer may support progression. However, presence non-small lung (NSCLC), which major cause death western countries, remains poorly defined. To fill this gap knowledge, we set up bank primary human fibroblasts isolated from both tumor-free regions and...