A5016496430- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Plant biochemistry and biosynthesis
- Microbial Natural Products and Biosynthesis
- Meat and Animal Product Quality
- Probiotics and Fermented Foods
- Listeria monocytogenes in Food Safety
- Salmonella and Campylobacter epidemiology
- Catalytic C–H Functionalization Methods
- Microbial Metabolites in Food Biotechnology
- Pharmaceutical and Antibiotic Environmental Impacts
- Protein Hydrolysis and Bioactive Peptides
- Pesticide and Herbicide Environmental Studies
- Polysaccharides and Plant Cell Walls
- Bacterial Genetics and Biotechnology
- Environmental Toxicology and Ecotoxicology
- Cellular Mechanics and Interactions
- Gut microbiota and health
- Enzyme Structure and Function
- Microbial Inactivation Methods
- Genetics, Aging, and Longevity in Model Organisms
- RNA and protein synthesis mechanisms
- Food composition and properties
- Analytical chemistry methods development
North Sichuan Medical University
2025
Singapore Institute of Technology
2023-2024
National University of Singapore
2016-2024
Lanzhou Petrochemical Polytechnic
2024
Hunan University
2024
Zhejiang University
2024
Xiangya Hospital Central South University
2024
Central South University
2024
Chinese Research Academy of Environmental Sciences
2023
China University of Geosciences
2023
Cells' biomechanical responses to external stimuli have been intensively studied but rarely implemented into devices that interact with the human body. We demonstrate hygroscopic and biofluorescent behaviors of living cells can be engineered design biohybrid wearables, which give multifunctional responsiveness sweat. By depositing genetically tractable microbes on a humidity-inert material form heterogeneous multilayered structure, we obtained films reversibly change shape biofluorescence...
Significance Shell biorefinery is an emerging concept that upcycles the major component of crustacean shell waste and like (chitin-rich) into value-added chemicals materials. To date, utilization waste-derived chitin as carbon nitrogen sources to produce nitrogen-containing (NCCs) via microbial fermentation has been underexplored. The lack a compatible pretreatment method in conjunction with customized NCC-producing strain placed impediment valorization effort. This study tackles this...
Abstract Production of renewable, high‐value N‐containing chemicals from lignocellulose will expand product diversity and increase the economic competitiveness biorefinery. Herein, we report a single‐step conversion furfural to pyrrole in 75 % yield as key building block, achieved via tandem decarbonylation–amination reactions over tailor‐designed Pd@S‐1 H‐beta zeolite catalytic system. Pyrrole was further transformed into dl ‐proline two steps following carboxylation with CO 2 hydrogenation...
The quality deterioration of tray-packed tilapia (genetically improved farmed strain Oreochromis niloticus) fillets stored at 0°C were studied by integrated evaluations sensory, microbiological, biochemical and physical analysis, in order to expound the mechanism fish spoilage develop most reliable indicators for assessment. results showed that four index asPseudomonas counts, total volatile basic nitrogen (TVBN), cadaverine (CAD) K value highly correlated (r > 0.90) with storage time...
Isoprenoids are natural products that all derived from isopentenyl diphosphate (IPP) and dimethylallyl (DMAPP). These precursors synthesized either by the mevalonate (MVA) pathway or 1-Deoxy-D-Xylulose 5-Phosphate (DXP) pathway. Metabolic engineering of microbes has enabled overproduction various isoprenoid DXP including lycopene, artemisinic acid, taxadiene levopimaradiene. To date, there is no method to accurately measure metabolic intermediates simultaneously so as enable identification...
Genetically engineered microbes have been intensively investigated as a means for the cost-effective production of isoprenoids. Bacillus subtilis is promising microbial host this purpose because its fast growth rate and GRAS (generally regarded safe) status. To date, development has impaired by lack genetic tools modulating expression multiple genes. In study, we present novel two-promoter system which can be used to independently control levels two gene cassettes over large dynamic range....
Significance This study used a genetic approach to probe the function of F-actin nucleating Arp2/3 complex in skin development. Although was expected be involved morphogenesis and cell adhesion, loss its activity did not result architectural problems epidermis. However, essential for epidermal function. We revealed two unexpected functions epidermis that are critical tissue physiology: tight junction assembly/function regulation YAP activity.
With the wide use of heavy metals as feed additives in animal production, little attention has been paid to metal resistance pathogenic bacteria. This study was performed investigate presence genes (HMRGs) Escherichia coli and Salmonella isolates its correlation with disinfectant (DRGs) antibiotic (ARGs). HMRGs 178 E. 294 isolated from chicken broiler farms retail meat were detected by PCR. Minimum inhibitory concentrations (MICs) determined broth microdilution method. The complete genome...
Improving catalytic ability of enzymes is critical to the success many metabolic engineering projects, but search space possible protein mutants too large explore exhaustively through experiments. To some extent, highly soluble tend exhibit high activity due their better folding quality. Here, we demonstrate that an optimization algorithm based on a regression model can effectively design short peptide tags improve solubility few enzymes. Based sequence information, support vector recently...