A5112411603- Cancer Genomics and Diagnostics
- Cardiomyopathy and Myosin Studies
- Prostate Cancer Treatment and Research
- Genomics and Phylogenetic Studies
- Prostate Cancer Diagnosis and Treatment
- Advanced Proteomics Techniques and Applications
- Cardiac electrophysiology and arrhythmias
- Bladder and Urothelial Cancer Treatments
- Mitochondrial Function and Pathology
- Diet and metabolism studies
- Phenothiazines and Benzothiazines Synthesis and Activities
- Reconstructive Surgery and Microvascular Techniques
- Adipose Tissue and Metabolism
- Pharmaceutical and Antibiotic Environmental Impacts
- Epigenetics and DNA Methylation
- Molecular Biology Techniques and Applications
- CRISPR and Genetic Engineering
- Microfluidic and Capillary Electrophoresis Applications
- Nanoparticles: synthesis and applications
- Genetic factors in colorectal cancer
- Mass Spectrometry Techniques and Applications
- Reconstructive Facial Surgery Techniques
The Prostate Centre
2023-2025
University of British Columbia
2016-2025
Genome British Columbia
2021
University of Tübingen
2016
Indianapolis Zoo
2016
The androgen receptor (AR) is a pivotal drug target for the treatment of prostate cancer, including its lethal castration-resistant (CRPC) form. All current non-steroidal AR antagonists, such as hydroxyflutamide, bicalutamide, and enzalutamide, binding site receptor, competing with endogenous androgenic steroids. Several mutations in this have been associated poor prognosis resistance to conventional cancer drugs. In order develop an effective CRPC therapy, it crucial understand effects...
The emergence of bacteria resistant to antibiotics and the resulting infections are increasingly becoming a public health issue. Multidrug-resistant (MDR) responsible for leading increased morbidity mortality in hospitals, prolonged time hospitalization, additional burden financial costs. Therefore, there is an urgent need novel antibacterial agents that will both treat MDR outsmart bacterial evolutionary mechanisms, preventing further resistance development. In this study, green synthesis...
Abstract A better understanding of the secondary injury mechanisms that occur after traumatic spinal cord (SCI) is essential for development novel neuroprotective strategies linked to restoration metabolic deficits. We and others have shown Ketogenic diet (KD), a high fat, moderate in proteins low carbohydrates improves behavioural outcomes rats with acute SCI. Ketones are alternative fuels mitochondrial ATP generation, can modulate signaling pathways via targeting specific receptors. Here,...
BACKGROUND: Protein-truncating mutations in the titin gene are associated with increased risk of atrial fibrillation. However, little is known about underlying pathophysiology. METHODS: We identified a heterozygous truncating variant (TTNtv) patient unexplained early onset fibrillation and normal ventricular function. generated patient-specific atrial- ventricular-like induced pluripotent stem cell-derived cardiomyocytes engineered heart tissue to evaluate impact TTNtv on electrophysiology,...
Neuroendocrine transdifferentiation (NEtD) of prostate adenocarcinoma (PRAD) leads to aggressive neuroendocrine cancer (NEPC). The LTL331 patient-derived xenograft (PDX) model consistently progresses NEPC following castration, mimicking clinical responses androgen-deprivation therapy. Here we tracked NEtD using longitudinal single-cell RNA sequencing (scRNA-seq) across eight time points in from pre- post-castration. Castration led the loss AR-high PRAD cells, expansion AR-low populations,...
Cancer is a complex disease that involves rapidly evolving cells, often forming multiple distinct clones. In order to effectively understand progression of patient-specific tumor, one needs comprehensively sample tumor DNA at time points, ideally obtained through inexpensive and minimally invasive techniques. Current sequencing technologies make the 'liquid biopsy' possible, which sampling patient's blood or urine circulating cell free (cfDNA). A certain percentage this originates from known...
To investigate global changes in ureters at the transcriptional, translational and functional levels, both while stents are indwelling after removal recovery, to study effects of targeting pathways that play a potential role.Pig were stented for varying amounts time (48 h, 72 14 days) impact on peristalsis, dilatation hydronephrosis assessed. RNAseq, proteomic, histological smooth muscle (SM) function analyses performed ureteric kidney tissues assess induced by stenting recovery. Pathway...
You have accessJournal of UrologyBladder Cancer: Invasive III1 Apr 2016MP49-01 NEXT GENERATION SEQUENCING OF CELL FREE DNA REVEALS GENOMIC ABERRATIONS IN METASTATIC UROTHELIAL CARCINOMA Tilman Todenhöfer, Stanislav Volik, Bernie Eigl, Scott North, Sonal Brahmbhatt, Anne Haegert, Johannes Mischinger, Arnulf Stenzl, Stephane LeBihan, Alexander Wyatt, Colin Collins, and Peter Black TodenhöferTilman Todenhöfer More articles by this author , VolikStanislav Volik EiglBernie Eigl NorthScott North...
Abstract Background Protein truncating mutations in the titin gene are associated with increased risk of atrial fibrillation (AF). However, little is known regarding underlying pathophysiology. Methods We identified a heterozygous variant patient unexplained early-onset AF using whole exome sequencing. used and ventricular induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs), CRISPR/Cas9 genetic correction, engineered heart tissue (EHT) constructs to evaluate impact on...
Abstract Optimizing the acquisition of proteomics data collected from a mass spectrometer (MS) requires careful selection processed material quantities, liquid-chromatography (LC) setup, and parameters. The small internal diameter (ID) columns standardly used in nano-chromatography coupled MS result long per injection overhead times that require sacrifices design offline-fractionation schemes. As cohort sizes numbers samples to be analyzed continue increase, there is need investigate methods...