A5073576128- Cell Adhesion Molecules Research
- Caveolin-1 and cellular processes
- S100 Proteins and Annexins
- Signaling Pathways in Disease
- Protease and Inhibitor Mechanisms
- Immune cells in cancer
- Cellular Mechanics and Interactions
- Glioma Diagnosis and Treatment
- Renal cell carcinoma treatment
- Nanoplatforms for cancer theranostics
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- Enzyme Structure and Function
- Liver physiology and pathology
- Enzyme function and inhibition
- TGF-β signaling in diseases
- Cellular transport and secretion
- Renal and related cancers
- Biochemical and Molecular Research
- Wnt/β-catenin signaling in development and cancer
- Cancer Cells and Metastasis
- Genetic and Kidney Cyst Diseases
- Phagocytosis and Immune Regulation
- Hippo pathway signaling and YAP/TAZ
- Angiogenesis and VEGF in Cancer
Cleveland Clinic
2011-2022
Boston Children's Hospital
2015
Harvard University
2015
University Hospitals Seidman Cancer Center
2012
Case Western Reserve University
2012
Case Comprehensive Cancer Center
2012
Cleveland Clinic Lerner College of Medicine
2011-2012
University of Wisconsin–Madison
2004-2008
Abstract Objective: Glioblastomas (GBMs) are lethal cancers that display cellular hierarchies parallel to normal brain. At the apex GBM stem cells (GSCs), which relatively resistant conventional therapy. Interactions with adjacent perivascular niche an important driver of malignancy and self‐renewal in GSCs. Extracellular matrix (ECM) cues instruct neural stem/progenitor cell–niche interactions, objective our study was elucidate its composition contribution GSC maintenance niche. Methods: We...
Abstract Therapeutic targeting of angiogenesis in glioblastoma has yielded mixed outcomes. Investigation tumor-associated focused on the factors that stimulate sprouting, migration, and hyperproliferation endothelial cells. However, little is known regarding processes underlying formation vessels. To address this issue, we investigated vessel CD31 + cells isolated from human tumors. The results indicate overexpression integrin α3β1 plays a central role promotion tube glioblastoma. Blocking...
Development of the metanephric kidney depends on tightly regulated interplay between self-renewal and differentiation a nephron progenitor cell (NPC) pool. Several key factors required for survival NPCs have been identified, including fibroblast growth factor (FGF) signaling transcription Wilms' tumor suppressor 1 (WT1). Here, we present evidence that WT1 modulates FGF by activating expression arrest-specific (Gas1), novel target gene modulator signaling. We show directly binds to conserved...
Abstract Activation of TNF receptor 1 (TNF-R1) can generate signals that promote either apoptosis or survival. In this study, we show these be determined by the character extracellular matrix in tumor microenvironment. Specifically, through studies glioblastoma, showed TNFα stimulation induced primary brain endothelial cells (EC) attached to collagen fibronectin (which engage integrins α2β1/α3β1 and α5β1, respectively), but did not induce ECs laminin engages α6β1 α3β1). TNF-R1 expression was...
The role of endogenously synthesized fibronectin (FN) in assembly was studied with cells lacking or expressing FN. Cells were cultured as homogeneous mixed populations on surfaces coated different matrix proteins. Compared FN-expressing cells, FN-null poorly assembled exogenous plasma FN (pFN) when adhered to vitronectin the recombinant cell-binding domain (III(7-10)) Vitronectin had a suppressive effect that overcome by co-adsorbed pFN laminin-1 but not soluble In co-cultures and endogenous...
Fibronectin-null cells assemble soluble fibronectin shortly after adherence to a substrate coated with intact but not when adherent the cell-binding domain of (modules (7)F3-(10)F3). Interactions regions adsorbed other than modules (7)F3-(10)F3, therefore, are required for early display cell surface sites that initiate and direct assembly.To identify these regions, coatings proteolytically derived or recombinant pieces containing in addition (7)F3-(10)F3 were tested effects on assembly by...
Anti-angiogenic therapy is a promising therapeutic approach for highly vascularized tumors including glioblastoma (GBM). Nonetheless, the efficacy of Bevacizumab - monoclonal antibody to VEGF-A limited only progression-free survival but not overall survival, due at least in part induction more invasive phenotype. Here, we investigated anti-tumor effect thrombospondin-1&2 mimetic peptide, ABT-898, on intracerebral xenografts derived from primary patient GBM neurospheres nude mouse. ABT-898...
<p>PDF file - 132K, Double-labeling of vWf and TNF�\, TNF-R1, or TNF-R2 in tumor-associated ECs GBM.</p>
<p>PDF file - 2.1MB, Double-labeling for cFLIP and vWf in endothelial cells of GBM normal brain samples</p>
<p>PDF file - 1MB, Absence of cFLIP expression in human GBM cells and stem plated on laminin</p>
<p>PDF file - 695K, Significantly increased mRNA Expression of TNF-R1 and TNF-R2 in GBM as compared to normal brain</p>
<p>PDF file - 658K, Characterization of the integrin receptors mediating attachment primary brain ECs to collagen, fibronectin and laminin.</p>
<p>PDF file - 79K</p>
<p>PDF file - 481K, Dose-dependent TNF�\�{induced apoptosis of primary brain ECs.</p>
<p>PDF file - 972K, Percent of ECs expressing integrin alpha 6 beta 1 versus 4.</p>
<p>PDF file - 2.1MB, Double-labeling for cFLIP and vWf in endothelial cells of GBM normal brain samples</p>
<p>PDF file - 79K</p>