A5022078964- RNA Research and Splicing
- Virus-based gene therapy research
- Genomics and Chromatin Dynamics
- Animal Virus Infections Studies
- RNA and protein synthesis mechanisms
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- Nuclear Structure and Function
- Bacteriophages and microbial interactions
- Herpesvirus Infections and Treatments
- SARS-CoV-2 detection and testing
- Cancer Research and Treatments
- Parasitic Diseases Research and Treatment
- Respiratory viral infections research
- Morphological variations and asymmetry
- Microbial infections and disease research
- CRISPR and Genetic Engineering
- CNS Lymphoma Diagnosis and Treatment
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- RNA Interference and Gene Delivery
- Glycosylation and Glycoproteins Research
- Developmental Biology and Gene Regulation
- Sarcoidosis and Beryllium Toxicity Research
- T-cell and Retrovirus Studies
Baylor College of Medicine
2019-2023
Center for Theoretical Biological Physics
2020-2023
Rice University
2020-2023
Kansas State University
2016
Guru Angad Dev Veterinary and Animal Sciences University
2012-2015
We investigated genome folding across the eukaryotic tree of life. find two types three-dimensional (3D) architectures at chromosome scale. Each type appears and disappears repeatedly during evolution. The architecture that an organism exhibits correlates with absence condensin II subunits. Moreover, depletion converts human to a state resembling seen in organisms such as fungi or mosquitoes. In this state, centromeres cluster together nucleoli, heterochromatin domains merge. propose...
Bovine respiratory disease (BRD) is the most costly affecting cattle industry. The pathogenesis of BRD complex and includes contributions from microbial pathogens as well host, environmental animal management factors. In this study, we utilized viral metagenomic sequencing to explore virome nasal swab samples obtained feedlot with acute asymptomatic pen-mates at six four feedlots in Mexico USA, respectively, April-October 2015. Twenty-one viruses were detected, bovine rhinitis A (52.7 %) B...
Early detection of SARS-CoV-2 infection is key to managing the current global pandemic, as evidence shows virus most contagious on or before symptom onset. Here, we introduce a low-cost, high-throughput method for diagnosing and studying infection. Dubbed Pathogen-Oriented Low-Cost Assembly & Re-Sequencing (POLAR), this amplifies entirety genome. This contrasts with typical RT-PCR-based diagnostic tests, which amplify only few loci. To achieve goal, combine enrichment developed by ARTIC...
Enhancers are key drivers of gene regulation thought to act via 3D physical interactions with the promoters their target genes. However, genome-wide depletions architectural proteins such as cohesin result in only limited changes expression, despite a loss contact domains and loops. Consequently, role contacts enhancer function remains debated. Here, we developed CRISPRi regulatory elements upon degron operation (CRUDO), novel approach measure how frequency impact effects on genes by...
Abstract Cohesin plays an essential role in chromatin loop extrusion, but its impact on a compartmentalized nuclear architecture, linked to functions, is less well understood. Using live-cell and super-resolved 3D microscopy, here we find that cohesin depletion human colon cancer derived cell line results endomitosis single multilobulated nucleus with chromosome territories pervaded by interchromatin channels. Chromosome contain domain clusters zonal organization of repressed domains the...
Abstract Early detection of infection with SARS-CoV-2 is key to managing the current global pandemic, as evidence shows virus most contagious on or before symptom onset. Here, we introduce a low-cost, high-throughput method for diagnosing and studying infection. Dubbed Pathogen-Oriented Low-Cost Assembly & Re-Sequencing (POLAR), this amplifies entirety genome. This contrasts typical RT-PCR-based diagnostic tests, which amplify only few loci. To achieve goal, combine enrichment developed...
Abstract Cohesin plays an essential role in chromatin loop extrusion, but its impact on a compartmentalized nuclear architecture, linked to functions, is debatable. Using live-cell and super-resolved 3D microscopy, we demonstrate that cohesin depleted cells pass through endomitosis rebuild single multilobulated nucleus (MLN) with chromosome territories (CTs) pervaded by interchromatin channels. CTs contain domain clusters zonal organization of repressed domains the interior transcriptionally...
Neoplastic infections due to oncogenic viruses are one of the major causes economic problems faced by poultry industry worldwide and present study was undertaken in order establish prevalence single or multiple avian associated virus apparently healthy chicken. Marek's disease (MDV), reticuloendotheliosis (REV) leukosis (ALV) could be detected from 9.23%, 95.38% 93.85% cases, respectively PCR applied on DNA extracted blood chickens. Mixed infection MDV+REV+ALV REV+ALV 9.23% 89.23%...