A5000316366- Monoclonal and Polyclonal Antibodies Research
- HER2/EGFR in Cancer Research
- Immunodeficiency and Autoimmune Disorders
- Biosimilars and Bioanalytical Methods
- Barrier Structure and Function Studies
- Immune Cell Function and Interaction
- Advanced Biosensing Techniques and Applications
- Phagocytosis and Immune Regulation
- Hedgehog Signaling Pathway Studies
- CAR-T cell therapy research
- Ferroptosis and cancer prognosis
- Gut microbiota and health
- Immunotherapy and Immune Responses
- Caveolin-1 and cellular processes
University Medical Center Utrecht
2022-2025
Heidelberg University
2024-2025
University Hospital Heidelberg
2024-2025
Glycosylated mucin proteins contribute to the essential barrier function of intestinal epithelium. The transmembrane MUC13 is an abundant glycoprotein with important functions for mucosal maintenance that are not yet completely understood. We demonstrate in human epithelial monolayers, localized both apical surface and tight junction (TJ) region on lateral membrane. deletion resulted increased transepithelial resistance (TEER) reduced translocation small solutes. TEER buildup ΔMUC13 cells...
The efficacy of immunotherapy in pancreatic ductal adenocarcinoma (PDAC) remains limited. tumor microenvironment (TME), characterized by the accumulation suppressive myeloid cells including neutrophils, attributes to resistance PDAC. IgA monoclonal antibodies (mAbs) can activate neutrophils kill cells; this be further enhanced blocking immune checkpoint CD47. In study, we investigated potential therapeutic strategy for We determined expression tumor-associated antigens (TAAs) on PDAC cell...
Abstract EGFR plays an essential role in cellular signaling pathways that regulate cell growth, proliferation, and survival is often dysregulated cancer. Several monoclonal IgG antibodies have been clinically tested over the years, which exert their function via blocking ligand binding domain (thereby inhibiting downstream signaling) inducing Fc-related effector functions, such as antibody-dependent cytotoxicity (ADCC) phagocytosis (ADCP). However, these do not optimally recruit neutrophils,...
<p>Supplementary Figure 2: Binding characteristics of clinical IgG EGFR antibodies.</p>
<p>Supplementary Figure 1: Purification of IgA3.0 EGFR mAbs.</p>
<div>Abstract<p>EGFR plays an essential role in cellular signaling pathways that regulate cell growth, proliferation, and survival is often dysregulated cancer. Several monoclonal IgG antibodies have been clinically tested over the years, which exert their function via blocking ligand binding domain (thereby inhibiting downstream signaling) inducing Fc-related effector functions, such as antibody-dependent cytotoxicity (ADCC) phagocytosis (ADCP). However, these do not optimally...
<p>Supplementary Figure 3: Tumor outgrowth in A431-luc2 tumor-bearing mice.</p>
<p>Supplementary Figure 7: Binding kinetics of IgA3.0 EGFR antibodies.</p>
<p>Supplementary Figure 5: Tumor outgrowth in A431-luc2 tumor-bearing mice treated with IgA3.0 Matuzumab.</p>
<p>Supplementary Figure 8: Internalization of IgA3.0 EGFR antibodies.</p>
<p>Supplementary Figure 1: Purification of IgA3.0 EGFR mAbs.</p>
<p>Supplementary Figure 4: IgA3.0 EGFR mitigated tumor burden in a short intraperitoneal EL4 model.</p>
<p>Supplementary Figure 2: Binding characteristics of clinical IgG EGFR antibodies.</p>
<p>Supplementary Figure 7: Binding kinetics of IgA3.0 EGFR antibodies.</p>
<p>Supplementary Figure 5: Tumor outgrowth in A431-luc2 tumor-bearing mice treated with IgA3.0 Matuzumab.</p>
<p>Supplementary Figure 8: Internalization of IgA3.0 EGFR antibodies.</p>
<p>Supplementary Figure 6: Efficacy of neutrophil depletion in the long i.p. A431-luc2 model.</p>
<p>Supplementary Figure 6: Efficacy of neutrophil depletion in the long i.p. A431-luc2 model.</p>
<p>Supplementary Figure 4: IgA3.0 EGFR mitigated tumor burden in a short intraperitoneal EL4 model.</p>
<p>Supplementary Figure 3: Tumor outgrowth in A431-luc2 tumor-bearing mice.</p>
<div>Abstract<p>EGFR plays an essential role in cellular signaling pathways that regulate cell growth, proliferation, and survival is often dysregulated cancer. Several monoclonal IgG antibodies have been clinically tested over the years, which exert their function via blocking ligand binding domain (thereby inhibiting downstream signaling) inducing Fc-related effector functions, such as antibody-dependent cytotoxicity (ADCC) phagocytosis (ADCP). However, these do not optimally...
Abstract Regulation and adaptation of intestinal epithelial barrier function is essential for human health. The transmembrane mucin MUC13 an abundant glycoprotein with important functions mucosal maintenance that are not yet completely understood. We demonstrate in monolayers localized to both the apical surface tight junction (TJ) region on lateral membrane. deletion resulted increased transepithelial resistance (TEER) reduced translocation small solutes. TJ proteins including claudins...