A5009250306- Liver Disease Diagnosis and Treatment
- Gut microbiota and health
- Diet and metabolism studies
- Muscle Physiology and Disorders
- Cellular transport and secretion
- Diet, Metabolism, and Disease
- Ubiquitin and proteasome pathways
- Metabolomics and Mass Spectrometry Studies
- Essential Oils and Antimicrobial Activity
- Cardiomyopathy and Myosin Studies
- Circadian rhythm and melatonin
- Plant biochemistry and biosynthesis
- Dietary Effects on Health
- Ion channel regulation and function
- Diabetes and associated disorders
- Silk-based biomaterials and applications
- Neurobiology and Insect Physiology Research
- Advanced Sensor and Energy Harvesting Materials
University of Wisconsin–Madison
2020-2024
Mechanical signals, such as those evoked by maximal-intensity contractions (MICs), can induce an increase in muscle mass. Rapamycin-sensitive signaling events are widely implicated the regulation of this process; however, recent studies indicate that rapamycin-insensitive also involved. Thus, to identify these events, we generate a map MIC-regulated and rapamycin-sensitive phosphoproteome. In total, quantify more than 10,000 unique phosphorylation sites find 2,000 significantly affected...
Skeletal muscle size is controlled by the balance between protein synthesis and degradation. Given essential role of skeletal in maintaining a high quality life, understanding mechanisms that modulate this are critical importance. Previously, we demonstrated muscle-specific knockout TRIM28 reduces function current study, discovered effect associated with an increase degradation dramatic reduction expression Mettl21c. Importantly, also determined overexpression Mettl21c sufficient to induce...
Summary Nonalcoholic fatty liver disease (NAFLD) is multifactorial in nature, affecting over a billion people worldwide. The gut microbiome has emerged as an associative factor NAFLD, yet mechanistic contributions are unclear. Here, we show fast food (FF) diets containing high fat, added cholesterol, and fructose/glucose drinking water differentially impact short- vs. long-term NAFLD severity progression conventionally-raised, but not germ-free mice. Correlation machine learning analyses...
Nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH) are hepatic manifestations of metabolic syndrome major indications for transplantation. Western diet contributes to pathogenesis, partially mediated through the gut microbiome, yet mechanisms remain elusive. Human epidemiological studies identified high dietary cholesterol intake as a NAFLD risk factor it is essential drive in murine models, little known about its role reshaping microbiota. Using fast food (FF)...
Circadian rhythms are ubiquitous in nature, driving many bodily processes and behaviors, including sleep-wake cycles feeding patterns over 24 hours. We others revealed gut microbes their functional outputs also exhibit diurnal that responsive to how much, what, when food is consumed. These microbial cues integrated into host circadian networks, serving as key regulators of metabolism. High fat (HF) diet disrupts oscillations, impacting diet-induced obesity (DIO). Apart from feeding, factors...
Mechanical signals, such as those which are evoked during maximal intensity contractions (MIC), can induce an increase in skeletal muscle size. It has been widely concluded that this process is driven by the activation of rapamycin‐sensitive / mTORC1‐dependent signaling; however, recent studies have revealed mTORC1‐independent signaling events might also be involved. Thus, effort to identify these events, we generated a comprehensive map MIC‐regulated, and phosphoproteomes. In total,...
Many biologically active natural products contain phenol groups that are prone to rapid phase‐II metabolism. In order better understand the structural basis for these metabolic transformations, we have investigated in vitro metabolism of rooperol, a bis‐catechol with anti‐cancer activity, and caffeic acid phenethyl amide (CAPA), synthetic analog product antioxidant cytoprotective activities. presence pig liver microsomes supplemented UDP‐glucuronic acid, both catechols undergo form mono‐...
Protein homeostasis plays a critical role in the regulation of skeletal muscle size, and maintenance size contributes significantly to disease prevention quality life. Over past few decades, it has become widely accepted that is controlled by net balance between rates protein synthesis degradation. Despite this assertion, mechanisms modulate lead changes remain incompletely defined. Nevertheless, advancements have been made. For instance, recent study from our lab revealed myofiber-specific...