A5046360268- Organ Transplantation Techniques and Outcomes
- Liver Disease Diagnosis and Treatment
- Liver physiology and pathology
- Liver Disease and Transplantation
- Renal Transplantation Outcomes and Treatments
- Organ Donation and Transplantation
- Xenotransplantation and immune response
- Virus-based gene therapy research
- Hepatitis C virus research
- Hepatocellular Carcinoma Treatment and Prognosis
- Viral gastroenteritis research and epidemiology
- Metabolism and Genetic Disorders
- Pancreatic function and diabetes
- CRISPR and Genetic Engineering
- Diet and metabolism studies
- Abdominal Surgery and Complications
- Tissue Engineering and Regenerative Medicine
- Hemodynamic Monitoring and Therapy
- Cutaneous Melanoma Detection and Management
- Drug-Induced Hepatotoxicity and Protection
- Esophageal and GI Pathology
- Advanced Radiotherapy Techniques
- Nail Diseases and Treatments
- Reproductive Health and Technologies
- Breast Lesions and Carcinomas
Massachusetts General Hospital
2025
Thomas Jefferson University Hospital
2021-2024
Thomas Jefferson University
2021-2023
Mayo Clinic
2013-2021
Johns Hopkins Medicine
2019
Johns Hopkins University
2018-2019
Mayo Clinic in Arizona
2012-2018
WinnMed
2015-2017
Mayo Clinic in Florida
2014
St. Luke's Hospital
2014
Purpose To investigate the utility of magnetic resonance (MR) elastography-derived mechanical properties in discrimination hepatic inflammation and fibrosis early stages chronic liver diseases. Materials Methods All studies were approved by institutional animal care use committee. A total 187 animals studied, including 182 mice five pigs. These represented different diseases with a varying combination extent inflammation, fibrosis, congestion, portal hypertension. Multifrequency...
Transplantation of gene-corrected autologous hepatocytes can cure metabolic disease in a preclinical pig model hereditary tyrosinemia type 1.
Hereditary tyrosinemia type I (HT1) is caused by deficiency in fumarylacetoacetate hydrolase (FAH), an enzyme that catalyzes the last step of tyrosine metabolism. The most severe form disease presents acutely during infancy, and characterized liver involvement, commonly resulting death if untreated. Generation FAH+/− pigs was previously accomplished adeno-associated virus-mediated gene knockout fibroblasts somatic cell nuclear transfer. Subsequently, these animals were outbred crossed to...
Abstract Background The opioid crisis has led to an increase in hepatitis C virus‐positive donors the past decade. Whereas historically seropositive organs were routinely discarded, advent of direct‐acting antiviral agents notably expanded utilization from with C. There been growing experience liver transplantation (LT) recipients. However, data remain limited on LT or ribonucleic acid positive seronegative Methods We performed a retrospective study 26 recipients who received donor livers...
Orthotopic liver transplantation remains the only curative therapy for inborn errors of metabolism. Given tremendous success primary immunodeficiencies using ex-vivo gene with lentiviral vectors, there is great interest in developing similar therapies metabolic diseases. We have previously generated a pig model hereditary tyrosinemia type 1 (HT1), an autosomal recessive disorder caused by deficiency fumarylacetoacetate hydrolase (FAH). Using this model, we demonstrated and cell vector to...
Publications using the ALPPS (associating liver partition and portal vein ligation for a staged hepatectomy) procedure have demonstrated future remnant growth of 40-160% in only 6-9 days. The present study aimed to develop describe first large animal model that can be used studies.A total 13 female domestic pigs underwent stage 1 (portal division parenchymal transection) followed by 2 (completion left-extended 7 days later. An abdominal computed tomography (CT) scan was performed immediately...
The Kidney Allocation System (KAS) has resulted in fewer pediatric kidneys being allocated to deceased donor kidney transplant (pDDKT) recipients. This had prompted concerns that post-pDDKT outcomes may worsen. To study this, we used SRTR data compare the of 953 pre-KAS pDDKT (age <18 years) recipients (December 4, 2012-December 3, 2014) with 934 post-KAS 2014-December 2016). We analyzed mortality and graft loss by using Cox regression, delayed function (DGF) logistic length stay (LOS)...
Cell transplantation is a potential treatment for the many liver disorders that are currently only curable by organ transplantation. However, one of major limitations hepatocyte (HC) an inability to monitor cells longitudinally after injection. We hypothesized thyroidal sodium iodide symporter (NIS) gene could be used visualize transplanted HCs in rodent model inherited disease: hereditary tyrosinemia type 1. Wild-type C57Bl/6J mouse were transduced ex vivo with lentiviral vector containing...
A barrier to using organs from hepatitis C virus (HCV)-viremic donors is the high cost of direct-acting antivirals (DAAs) and concerns about access for recipients after transplantation. The purpose this study was evaluate access, cost, timing HCV DAAs following transplantation.This a single-center, retrospective HCV-negative adult transplant June 2017 December 2019 who received grafts HCV-viremic and/or HCV-seropositive individuals became viremic transplantation.Between 2019, there were 60...
Kidney congestion is a common pathophysiologic pathway of acute kidney injury (AKI) in sepsis and heart failure. There no noninvasive tool to measure intracapsular pressure (KIP) directly.We evaluated the correlation KIP with elasticity measured by ultrasound surface wave elastography (USWE). We directly transcatheter three pigs at baseline after bolus infusion normal saline, norepinephrine, vasopressin, dopamine, fenoldopam; infiltration 2-L peritoneal dialysis solution intra-abdominal...
The recent shortage of the University Wisconsin (UW) solution prompted increased utilization histidine-tryptophan-ketoglutarate (HTK) for liver graft preservation. This contemporary study analyzed deceased donor transplant outcomes following preservation with HTK vs UW. Patients receiving transplantations between January 1, 2019, and June 30, 2022, were retrospectively identified utilizing Organ Procurement Transplant Network database, stratified by UW, a propensity score matching analysis...
Gene therapy is an ideal choice to cure many inborn errors of metabolism the liver. Ex-vivo, lentiviral vectors have been used successfully in treatment hematopoietic diseases humans, as their use offers stable transgene expression due vector's ability integrate into host genome. This method demonstrates application ex vivo gene hepatocytes a large animal model hereditary tyrosinemia type I. process consists 1) isolation primary from autologous donor/recipient animal, 2) delivery via...
Liver transplantation continues to face significant organ shortages and efficient utilization of marginal donors is paramount. This study evaluates the practice patterns outcomes in liver when utilizing allografts from who required extracorporeal membrane oxygenation (ECMO) support. We performed a retrospective review Gift Life (PA, NJ, DE) organ-procuring organization database for transplants using supported on ECMO nondonation purposes. These were cross-referenced transplant recipients...
The mammalian liver's regenerative ability has led researchers to engineer animals as incubators for expansion of human hepatocytes. properties hepatocytes in immunodeficient mice are well known. However, little been reported about larger that more scalable and practical clinical purposes. Therefore, we engineered swine support identify barriers their application. Immunodeficient were by knockout the recombinase-activating gene 2 (RAG2) fumarylacetoacetate hydrolase (FAH). Immature (ihHCs)...
Cell-based therapies for liver disease rely on a high-quality supply of hepatocytes and means storage during transportation from site isolation to usage. Unfortunately, frozen cryopreservation is associated with unacceptable loss hepatocyte viability after thawing. The purpose this study was optimize conditions cold rat spheroids without freezing. Rat were isolated by two-step perfusion method; formed 48 h rocked culture in serum-free medium (SFM). Spheroids then maintained at 37 °C (control...
Non-alcoholic steatohepatitis (NASH)-related hepatocellular carcinoma (HCC) has become the second leading cause of HCC-related liver transplantation in United States. This study investigated post-transplant recurrence and survival for patients transplanted NASH-related HCC compared to non-NASH etiologies. Retrospective review Network Organ Sharing (UNOS) Procurement Transplantation (OPTN) database identified 7,461 with HCC—1,405 underlying NASH 6,086 diseases. After propensity score matching...