A5102239689- Advanced Proteomics Techniques and Applications
- Electrochemical sensors and biosensors
- Analytical Chemistry and Sensors
- Mass Spectrometry Techniques and Applications
- Pregnancy and Medication Impact
- Cancer Risks and Factors
- Machine Learning in Bioinformatics
- Analytical Chemistry and Chromatography
- Analytical Methods in Pharmaceuticals
- Chemical Synthesis and Analysis
- Pregnancy and preeclampsia studies
University of Michigan
2024
Ain Shams University
2016-2024
Assessment of drug transport across the placenta is important in understanding effect drugs on placental and fetal health. These phenomena can be studied both vitro cell lines ex vivo perfusions. We have successfully developed a sensitive yet simple high performance liquid chromatography (HPLC) method coupled with fluorescence detection to determine concentration doxorubicin (DXR) culture media for studies human trophoblast cells (BeWo, b30 clone) perfusion experiments. The was based protein...
Tyrosine sulfation, an understudied but crucial post-translational modification, cannot be directly detected in conventional nanoflow liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) due to the extreme sulfate lability. Here, we report detection of sulfate-retaining fragments from LC-electron capture dissociation (ECD) and nanoLC-electron transfer higher energy collision (EThcD). Sulfopeptide candidates were identified by Proteome Discoverer MSFragger analysis nanoLC-HCD MS/MS...
Tyrosylprotein sulfotransferases (TPST1 and TPST2) are only known to exist in the Golgi. Thus, Yu et al.'s findings (Nat. Chem. Biol. 2023; https://doi.org/10.1038/s41589-023-01267-9) that histone H3 sulfation occurs cytosol surprising. Cytosolic sulfotransferase SULT1B1, which sulfates phenolic small molecules, was proposed catalyze H3Y99 with 3'-phosphoadenosine 5'-phosphosulfate (PAPS) as SO3 donor. Bottom-up liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomic...