A5002492154- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Biomedical Ethics and Regulation
- Renal and related cancers
University of Wisconsin–Madison
2017-2024
Wisconsin Institutes for Discovery
2017-2018
Human pluripotent stem cell (hPSC)-derived neural organoids display unprecedented emergent properties. Yet in contrast to the singular neuroepithelial tube from which entire central nervous system (CNS) develops vivo, current organoid protocols yield tissues with multiple units, a.k.a. rosettes, each acting as independent morphogenesis centers and thereby confounding coordinated, reproducible tissue development. Here, we discover that controlling initial morphology can effectively (>80%)...
SUMMARY Neural organoids have revolutionized how human neurodevelopmental disorders (NDDs) are studied. Yet, their utility for screening complex NDD etiologies and in drug discovery is limited by a lack of scalable quantifiable derivation formats. Here, we describe the RosetteArray ® platform’s ability to be used as an off-the-shelf, 96-well plate assay that standardizes incipient forebrain spinal cord organoid morphogenesis micropatterned, 3-D, singularly polarized neural rosette tissues...
Abstract Human pluripotent stem cell (hPSC)-derived neural organoids have revolutionized in vitro modelling of human neurological disorders. Cell-intrinsic morphogenesis processes displayed within these tissues could serve as the basis for ex vivo manufacture brain and spinal cord with biomimetic anatomy physiology. However, we must first understand how to control their emergent properties starting at genesis organoid formation, i.e. emergence polarized neuroepithelium. In vivo, all CNS...