A5085454661- Neuroscience and Neural Engineering
- Advanced Memory and Neural Computing
- Neural dynamics and brain function
- EEG and Brain-Computer Interfaces
- Nanowire Synthesis and Applications
- 3D Printing in Biomedical Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Bio-sensing Technologies
- CCD and CMOS Imaging Sensors
- Electrical and Bioimpedance Tomography
- Microfluidic and Capillary Electrophoresis Applications
- Muscle activation and electromyography studies
- Carbon Nanotubes in Composites
- Advancements in Semiconductor Devices and Circuit Design
- Network Time Synchronization Technologies
- Conducting polymers and applications
- Mechanical and Optical Resonators
- Embedded Systems Design Techniques
- Analytical Chemistry and Sensors
ETH Zurich
2013-2019
Studies on information processing and learning properties of neuronal networks would benefit from simultaneous parallel access to the activity a large fraction all neurons in such networks. Here, we present CMOS-based device, capable simultaneously recording electrical over thousand cells vitro The device provides sufficiently high spatiotemporal resolution enable, at same time, preparations subcellular, cellular, network level. key feature is rapidly reconfigurable array 26 400...
To advance our understanding of the functioning neuronal ensembles, systems are needed to enable simultaneous recording from a large number individual neurons at high spatiotemporal resolution and good signal-to-noise ratio. Moreover, stimulation capability is highly desirable for investigating, example, plasticity learning processes. Here, we present microelectrode array (MEA) system on single CMOS die in vitro stimulation. The incorporates 26,400 platinum electrodes, fabricated by in-house...
Biological cells are characterized by highly complex phenomena and processes that are, to a great extent, interdependent. To gain detailed insights, devices designed study cellular need enable tracking manipulation of multiple cell parameters in parallel; they have provide high signal quality high-spatiotemporal resolution. this end, we developed CMOS-based microelectrode array system for vitro applications integrates six measurement stimulation functions, the largest number date. Moreover,...
Microfluidic hanging-drop networks enable culturing and analysis of 3D microtissue spheroids derived from different cell types under controlled perfusion investigating inter-tissue communication in multi-tissue formats. In this paper we introduce a compact on-chip pumping approach for flow control networks. The pump includes one pneumatic chamber located directly above the hanging drops uses surface tension at liquid-air-interface actuation. Control protocol provides wide range...
A monolithic multi-functional CMOS microelectrode array system was developed that enables label-free electrochemical impedance spectroscopy of cells in vitro at high spatiotemporal resolution. The electrode includes 59,760 platinum microelectrodes, densely packed within a 4.5 mm × 2.5 sensing region pitch 13.5 μm. total 32 on-chip lock-in amplifiers can be used to measure the any arbitrarily chosen subset electrodes array. sinusoidal voltage, generated by an waveform generator with frequency...
Various CMOS-based micro-electrode arrays (MEAs) have been developed in recent years for extracellular electrophysiological recording/stimulation of electrogenic cells [1–5]. Mostly two approaches used: (i) the active-pixel approach (APS) [2–4], which features simultaneous readout all electrodes, however, at expense a comparably high noise level, and (ii) switch-matrix (SM) approach, yields better performance, whereas only subset electrodes (e.g.,1024) is simultaneously read out [5]. All...
We present a monolithic complementary metal-oxide semiconductor (CMOS)-based sensor system comprising an array of silicon nanowire field-effect transistors (FETs) and the signal-conditioning circuitry on same chip. The nanowires were fabricated by chemical vapor deposition methods then transferred to CMOS chip, where Ti/Pd/Ti contacts had been patterned via e-beam lithography. on-chip measures current flowing through each FET upon applying constant source-drain voltage. analog signal is...
A monolithic measurement platform was implemented to enable label-free in-vitro electrical impedance spectroscopy measurements of cells on multi-functional CMOS microelectrode array. The array includes 59,760 platinum microelectrodes, densely packed within a 4.5 mm × 2.5 sensing region at pitch 13.5 μm. 32 on-chip lock-in amplifiers can be used measure the any arbitrarily chosen electrodes by applying sinusoidal voltage, generated an waveform generator with frequency range from 1 Hz MHz, and...
We present novel voltage stimulation buffers with controlled output current, along recording circuits featuring adjustable high-pass cut-off filtering to perform efficient while actively suppressing artifacts in high-density microelectrode arrays. Owing the dense packing and close proximity of electrodes such systems, a through one electrode can cause large electrical on neighboring that easily saturate corresponding amplifiers. To suppress artifacts, corner frequencies all available 2048...
Here, we present 2048 low-noise, low-offset, and low-power action-potential recording channels, integrated in a multi-functional high-density microelectrode array. A resistively loaded open-loop topology has been adapted for the first-stage amplifier to achieve 2.4 μVrms noise levels at low power consumption. Two novel pseudo-resistor structures have used realize very HPF corner frequencies with small variations across all channels. The adjustability of pseudo resistors exploited "soft"...
We present a CMOS-based high-density microelectrode array (HD-MEA) system that enables mapping of brain slices in-vitro with multiple readout modalities. The 4.48×2.43 mm2 consists 59,760 micro-electrodes at 13.5 µm pitch (5487 electrodes/mm2). overall features 2048 action-potential, 32 local-field-potential and current recording channels, impedance-measurement 28 neurotransmitter-detection channels 16 voltage/current stimulation channels. real-time label-free monitoring position, size,...
We present a highly configurable, high-temporal-and-spatial-resolution microelectrode array (MEA) system, fabricated in CMOS technology, featuring 26'400 platinum microelectrodes (5×9 μm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> ) with pitch of 17.5 μm, arranged rectangular grid within an overall area 3.8×2.1 mm . The constitutes very flexible bidirectional interface to electrogenic cells. High-throughput, subcellular-resolution...
We present a new on-chip pumping approach for microfluidic hanging-drop networks that are used experiments with 3D microtissue spheroids. The pump includes pneumatic chamber located directly above one of the hanging drops and uses liquid-air-interface flow-control. With this several independent drop (HDN) can be operated in parallel only single actuation line. concept enables closed-loop medium circulation between different organ models body-on-a-chip applications allows multiple...
Event Abstract Back to Multi-Functional CMOS-HDMEA Integrating 59'760 Electrodes, 2'048 Electrophysiology Readouts, Stimulation Units, Impedance and Neurotransmitter Measurement Units Jelena Dragas1*, Vijay Viswam2, Amir Shadmani1, Yihui Chen3, Alexander Stettler1, Milos Radivojevic1, Jan Müller1 Andreas Hierlemann1 1 ETH Zurich, Department of Biosystems Science Engineering, Switzerland 2 3 Motivation In order study the electrophysiological properties electrogenic cells, state-of-the art...
Event Abstract Back to A Novel Multi-Functional High-Density MEA Platform for Studying Electrogenic Cells Amir Shadmani1, Vijay Viswam2, Jelena Dragas2, Yihui Chen3, Raziyeh Bounik3, Alexander Stettler2, Milos Radivojevic2, Jan Müller2 and Andreas Hierlemann2* 1 ETH Zürich, Department of Biosystems Science Engineering, Switzerland 2 Zurich, 3 Motivation In currently available high-density microelectrode arrays (HD-MEA), large number electrophysiological recording amplifiers are integrated...
An integrated measurement platform was implemented to make in-vitro impedance measurements of neuronal cells on a multi-functional CMOS microelectrode array (MEA). This high-density is made 59'760 platinum microelectrodes with pitch 13.5μm. Impedance sensing can be performed through 32 electrodes in parallel, while the electrode sites arbitrarily chosen array. A wave generator also chip perform spectroscopy within wide frequency range from 1Hz 1MHz. For proof concept, we show magnitude and...
This work presents a highly versatile and scalable analytical platform for 3D microtissues. The device combines microfluidics precise control of culture conditions with sensor technology parallel real-time monitoring microtissue metabolism. is based on an inverted, open microfluidic network. Interconnected hanging drops are formed underneath patterned glass chip, which hosts the integrated microelectrode array. electrodes can be individually functionalized used as biosensors. fabrication...