A5034689165- Photoreceptor and optogenetics research
- Retinal Development and Disorders
- Biochemical Analysis and Sensing Techniques
- Olfactory and Sensory Function Studies
- Receptor Mechanisms and Signaling
- RNA and protein synthesis mechanisms
- Photochromic and Fluorescence Chemistry
- Advanced Chemical Sensor Technologies
- Neuroscience and Neuropharmacology Research
- Circadian rhythm and melatonin
- Primate Behavior and Ecology
- Porphyrin and Phthalocyanine Chemistry
- Neurobiology and Insect Physiology Research
- bioluminescence and chemiluminescence research
- Regulation of Appetite and Obesity
- Animal Behavior and Reproduction
- Genetic Syndromes and Imprinting
- Chromosomal and Genetic Variations
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Metal-Catalyzed Oxygenation Mechanisms
- melanin and skin pigmentation
- Gastric Cancer Management and Outcomes
- Chemical Synthesis and Analysis
- Aquatic Ecosystems and Biodiversity
Kyoto University
2016-2025
Tohoku University
2023-2025
Tohoku University Hospital
2022-2025
Molecular Biology Consortium
2023
Institute of Primate Research
2011-2021
Inuyama Chuo Hospital
2010-2017
Red Cross Hospital
2015
The Graduate University for Advanced Studies, SOKENDAI
2015
University of Coimbra
2012
Japan Science and Technology Agency
2001-2007
Abstract Fibroblast growth factor receptor ( FGFR ) alterations drive oncogenesis in multiple tumor types. Here we studied pemigatinib, a selective, potent, oral FGFR1–FGFR3 inhibitor, the phase 2 FIGHT-207 basket study of FGFR- altered advanced solid tumors. Primary end points were objective response rate (ORR) cohorts A (fusions/rearrangements, n = 49) and B (activating non-kinase domain mutations, 32). Secondary progression-free survival, duration overall survival B, safety. Exploratory...
Movement of the ligand/receptor complex in rhodopsin (Rh) has been traced. Bleaching diazoketo (DK-Rh) containing 11- cis -3-diazo-4-oxo-retinal yields batho-, lumi-, meta-I-, and meta-II-Rh intermediates corresponding to those native Rh but at lower temperatures. Photoaffinity labeling DK-Rh these bleaching shows that ionone ring cross-links tryptophan-265 on helix F batho-Rh, alanine-169 D intermediates. It is likely movements involving a flip-over chromophoric trigger changes cytoplasmic...
Divergent natural selection acting on ecological traits, which also affect mate choice, is a key element of speciation theory, but has not previously been demonstrated at the molecular gene level to our knowledge. Here we demonstrate parallel evolution in two cichlid genera under strong divergent that affects both. Strong fixed opsin proteins with different predicted light absorbance properties opposite ends an environmental gradient. By expressing them and measuring absorbance, show...
Umami taste perception in mammals is mediated by a heteromeric complex of two G-protein-coupled receptors, T1R1 and T1R3. T1R1/T1R3 exhibits species-dependent differences ligand specificity; human specifically responds to l-Glu, whereas mouse more strongly other l-amino acids than l-Glu. The mechanism underlying this species difference remains unknown. In study we analyzed chimeric human-mouse receptors point mutants identified 12 key residues that modulate amino acid recognition the human-...
New genes contribute substantially to adaptive evolutionary innovation, but the functional evolution of new mammalian has been little explored at a broad scale. Previous work established mRNA-derived gene duplicates, known as retrocopies, models for study origination. Here we combine transcriptomic and epigenomic data unveil processes underlying stripped-down retrocopies into complex genes. We show that although some robustly expressed are transcribed from preexisting promoters, most evolved...
While olfaction is one of the most important senses in terrestrial mammals, it absent modern toothed whales (Odontoceti, Cetacea). Furthermore, behavioral evidence suggests that gustation very limited. In contrast, their aquatic sistergroup, baleen (Mysticeti) retain small but functional olfactory organs, and nothing known about gustation. It difficult to investigate mysticete chemosensory abilities because experiments a controlled setting are impossible.Here, we use regionalization bulb...
Summary Foregut fermentation is well known to occur in a wide range of mammalian species and single bird species. Yet, the foregut microbial community free‐ranging, foregut‐fermenting monkeys, that is, colobines, has not been investigated so far. We analysed microbiomes four free‐ranging proboscis monkeys ( Nasalis larvatus ) from two different tropical habitats with varying plant diversity (mangrove riverine forests), an individual semi‐free‐ranging setting supplemental feeding, captivity,...
Abstract Understanding how organisms adapt to their environments is a central question in evolutionary biology. Limestone langurs are unique among primates, as they exclusively found karst limestone habitats and have evolved mechanisms tolerate high levels of mineral ions, which typically associated with metal toxicity affecting organs, cells, genetic material. We generated high-quality reference genome (Tfra_5.0) for the langur (Trachypithecus francoisi), along re-sequencing data 48...
The visual transduction processes in rod and cone photoreceptor cells begin with photon absorption by the different types of pigments. Cone pigments exhibit faster regeneration from 11- cis -retinal opsin decay physiologically active intermediate (meta II) than does pigment, rhodopsin, as expected, due to functional difference between cells. To identify amino acid residue(s) responsible for molecular properties pigments, we selected three positions (64, 122, 150), where have residues...
Many examples of the appearance similar traits in different lineages are known during evolution organisms. However, underlying genetic mechanisms have been elucidated very few cases. Here, we provide a clear example evolutionary parallelism, involving changes same pathway, providing functional adaptation RH1 pigments to deep-water habitats adaptive radiation East African cichlid fishes. We determined sequences from 233 individual cichlids. The reconstruction with 11- cis -retinal 28 showed...
Macaca fascicularis aurea ( Mfa ) is the only macaque which has been recorded to use stone tools access encased foods. They live in close contact with M. Mff southwestern Thailand and hybrids were reported [Fooden, 1995]. Although can be seen same habitat types, tool‐use behavior never . Thus, comparing morphological characteristics genetics between should help elucidate not differences genetic divergence these subspecies but also potentially relationship their tool behavior. We surveyed...
Although recent studies revealed chondroitinase ABC (ChABC), an enzyme that degrades chondroitin sulfate proteoglycans, promotes CNS regeneration in vivo, the usefulness of its application for transplantation is not clear. We investigated if treatment with ChABC can promote synapse formation between graft and host neurons following retinal transplantation. Dissociated cells were prepared from neonatal Nrl-GFP transgenic mice which rod photoreceptors their progenitor are labeled GFP. Each...
Imprinted genes are expressed from a single parental allele, with the other allele often silenced by DNA methylation (DNAme) established in germline. While species-specific imprinted orthologues have been documented, molecular mechanisms underlying evolutionary switch biallelic to expression unknown. During mouse oogenesis, gametic differentially methylated regions (gDMRs) acquire DNAme transcription-guided manner. Here we show that oocyte transcription initiating lineage-specific endogenous...
Atypical neurodevelopment in autism spectrum disorder is a mystery, defying explanation despite increasing attention. We report on Japanese macaque that spontaneously exhibited autistic traits, namely, impaired social ability as well restricted and repetitive behaviors, along with our single-neuron genomic analyses. Its was measured turn-taking task, where two monkeys monitor each other's actions for adaptive behavioral planning. In its brain, the medial frontal neurons responding to others'...
Taste perception plays an essential role in food selection. Umami (savory) tastes are sensed by a taste receptor complex, T1R1/T1R3, that detects proteinogenic amino acids.1 High sensitivity to l-glutamate (l-Glu) is characteristic of human but the T1R1/T1R3 other vertebrates does not consistently show this l-Glu response.1,2 Here, we demonstrate derived state has evolved repeatedly large primates rely on leaves as protein sources, after their divergence from insectivorous ancestors....
Signal transduction in rod cells begins with photon absorption by rhodopsin and leads to the generation of an electrical response. The response profile is determined molecular properties phototransduction components. To examine how correlate rod-response profile, we have generated a knock-in mouse replaced its E122Q mutant, which exhibits different from those wild-type (WT) rhodopsin. Knock-in rods exhibited photosensitivity about 70% WT. Correspondingly, their single-photon had amplitude...
Rod and cone photoreceptor cells that are responsible for scotopic photopic vision, respectively, exhibit photoresponses different from each other contain similar phototransduction proteins with distinctive molecular properties. To investigate the contribution of properties visual pigments to responses cells, we have generated knock-in mice in which rod pigment (rhodopsin) was replaced mouse green-sensitive (mouse green). The green successfully transported outer segments, though expression...
Reproductive isolation that prevents interspecific hybridization between closely related coexisting species maintains sympatric diversity. One of the reproductive isolations is mate choice based on color signals (breeding perceived by vision). This well known in several animal taxa, yet little about its genetic and molecular mechanism. Lake Victoria cichlid fishes are thought to be an example In inhabiting different light environments rocky shore, speciation sensory drive through has been...
Abstract Background: Fibroblast growth factor receptor (FGFR) alterations are promising targets in different tumors. We report results of FIGHT-207, an open-label, single-arm phase 2 study pemigatinib, a potent, selective FGFR1-3 inhibitor, patients with previously treated unresectable or metastatic FGFR-altered solid tumors (NCT03822117). Methods: Patients were assigned to cohorts A (FGFR1-3 fusions), B activating non-kinase domain mutations), and C kinase mutations unknown significance)...
Chicken green is a visual pigment present in chicken green-sensitive cones and has an amino acid sequence more similar than any other cone pigments to the rod pigments, rhodopsins. Here we have investigated molecular properties of compared them with those rhodopsin elucidate whether or not rhodopsin-like pigment. While extinction coefficient photosensitivity very rhodopsin, it displays faster regeneration from 11-cis-retinal opsin formation decay physiologically active meta II intermediate...
In mammals, bitter taste is mediated by TAS2R genes, which belong to the large family of seven transmembrane G protein-coupled receptors. Because TAS2Rs are directly involved in interaction between mammals and their dietary sources, it likely that these genes evolved reflect species-specific diets during mammalian evolution. Here, we investigated sensitivities TAS2R16s various primates using a cultured cell expression system, found sensitivity each primate species varied according ligand....
The guppy (Poecilia reticulata) shows remarkable variation of photoreceptor cells in the retina, especially those sensitive to middle-to-long wavelengths light. Microspectrophotometry (MSP) has revealed varying "green", "green-yellow" and "yellow" cone among guppies Trinidad Venezuela (Cumana). In genome, there are four "long-wave" opsin loci (LWS-1, -2, -3 -4). Two LWS-1 alleles have potentially differing spectral sensitivity (LWS-1/180Ser LWS-1/180Ala). addition, two "middle-wave" (RH2-1...