A5088336448- Angiogenesis and VEGF in Cancer
- Lymphatic System and Diseases
- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- Body Contouring and Surgery
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Wound Healing and Treatments
- Cancer Cells and Metastasis
- Systemic Sclerosis and Related Diseases
- Protease and Inhibitor Mechanisms
- Parasitic Diseases Research and Treatment
- Planarian Biology and Electrostimulation
- interferon and immune responses
- Vascular Malformations and Hemangiomas
- Epigenetics and DNA Methylation
- Immunotherapy and Immune Responses
- Sympathectomy and Hyperhidrosis Treatments
- Cancer, Hypoxia, and Metabolism
- Congenital heart defects research
- Cytokine Signaling Pathways and Interactions
- Photodynamic Therapy Research Studies
- Platelet Disorders and Treatments
- Kruppel-like factors research
- Spine and Intervertebral Disc Pathology
University of Chicago
2015-2022
École Polytechnique Fédérale de Lausanne
2010-2021
Université de Bordeaux
2010-2013
Inserm
2007-2013
Uppsala University
1999-2009
Uppsala University Hospital
2005
Jagiellonian University
1986-2001
Toward Successful Tissue Repair The therapeutic use of growth factors in tissue regeneration has suffered from safety and efficacy issues. Reasoning that the unmet potential may be because nonphysiological delivery, Martino et al. (p. 885 ) engineered to bind strongly extracellular matrix proteins. These variants were able induce superior repair, compared wild-type Furthermore, unwanted side effects decreased: For example, angiogenic factor VEGF showed reduced vascular permeability, a...
Rationale: Lymphatic transport of peripheral interstitial fluid and dendritic cells (DCs) is important for both adaptive immunity maintenance tolerance to self-antigens. drainage can change rapidly dramatically on tissue injury or inflammation, therefore increased flow may serve as an early cue inflammation; however, the effects transmural lymphatic function are unknown. Objective: Here we tested hypothesis that lymph regulates cell functions endothelium. Methods Results: Using in vitro vivo...
Rationale: The transport of interstitial fluid and solutes into lymphatic vessels is important for maintaining homeostasis delivering antigens soluble factors to the lymph node immune surveillance. Transendothelial across endothelial cells (LECs) commonly considered occur paracellularly, or between cell–cell junctions, driven by local pressure concentration gradients. However, emerging evidence suggests that LECs also play active roles in regulating solute balance can scavenge store...
Objective— Perivascular cells, including pericytes, macrophages, smooth muscle and other specialized cell types, like podocytes, participate in various aspects of vascular function. However, aside from the well-established roles cells contributions vascular-associated are poorly understood. Our goal was to ascertain function perivascular macrophages adult tissues under nonpathological conditions. Approach Results— We combined confocal microscopy, vivo depletion, vitro assays investigate...
Lymphangiogenesis occurs in inflammation and wound healing, yet its functional roles these processes are not fully understood. Consequently, clinically relevant strategies for therapeutic lymphangiogenesis remain underdeveloped, particularly using growth factors. To achieve controlled, local capillary with protein engineering determine effects on fluid clearance, leukocyte trafficking, we developed a fibrin-binding variant of vascular endothelial factor C (FB-VEGF-C) that is slowly released...
Lymphatic endothelial cells (LECs) chemoattract naïve T and promote their survival in the lymph nodes, can cross-present antigens to CD8+ drive proliferation despite lacking key costimulatory molecules. However, functional consequence of LEC priming is unknown. Here, we show that while many proliferating LEC-educated enter early apoptosis, remainders comprise a long-lived memory subset, with transcriptional, metabolic, phenotypic features central stem cell-like cells. In vivo, these...
Visualizing the dynamic behaviors of immune cells in living tissue has dramatically increased our understanding how interact with their surroundings, contributing important insights into mechanisms leukocyte trafficking, tumor cell invasion, and T education by dendritic cells, among others. Despite substantial advances various intravital imaging techniques including two-photon microscopy generation multitudes reporter mice, there is a growing need to assess interactions context specific...
Abstract The angiogenesis inhibitor sunitinib is a tyrosine kinase that acts mainly on the VEGF and PDGF pathways. We have previously shown sequestered in lysosomes of exposed tumor endothelial cells. This phenomenon part drug-induced resistance observed clinic. Here, we demonstrate when to light, causes immediate destruction lysosomes, resulting release cell death. hypothesized this photoactivation could be used as vaso-occlusive vascular-targeting approach treating cancer. Spectral...
The common experimental use of B16-F10 melanoma cells focuses on exploring their metastatic potential following intravenous injection into mice. In this study, are used to develop a primary tumor model by implanting them directly the ears C57BL/6J represents reproducible and easily traceable tool for local growth making additional in vivo observations, due localization tumors. This is relatively simple involves (i) surgical opening ear skin, (ii) removal square-piece cartilage followed (iii)...
Lymphatic vessels transport fluid, antigens, and immune cells to the lymph nodes orchestrate adaptive immunity maintain peripheral tolerance. Lymphangiogenesis has been associated with inflammation, cancer metastasis, autoimmunity, tolerance transplant rejection, thus, targeted lymphatic ablation is a potential therapeutic strategy for treating or preventing such events. Here we define conditions that lead specific local closure of vasculature using photodynamic therapy (PDT)....
Therapeutic regulation of tissue vascularization has appeared as an attractive approach to treat a number human diseases. In vivo neovascularization assays that reflect physiological and pathological formation neovessels are important in this effort. report we present assay where the effects activators inhibitors angiogenesis can be quantitatively qualitatively measured. A provisional matrix composed collagen I fibrin was formed plastic cylinder implanted onto chick chorioallantoic membrane....
Clinical manifestations of severe COVID-19 include coagulopathies that are exacerbated by the formation neutrophil extracellular traps (NETs). Here, we report pulmonary lymphatic vessels, which traffic neutrophils and other immune cells to lung-draining lymph node (LDLN), can also be blocked fibrin clots in COVID-19. Immunostained tissue sections from decedents revealed widespread clotting not only lung but LDLN, where extent correlated with presence abnormal, regressed, or missing germinal...
In order to map the extracellular or membrane proteome associated with vasculature and stroma in an embryonic organism vivo, we developed a biotinylation technique for chicken embryo combined it mass spectrometry bioinformatic analysis. We also applied this procedure implanted tumors growing on chorioallantoic after induction of granulation tissue. Membrane matrix proteins were most abundant components identified. Relative quantitative analysis revealed differential protein expression...
Lymphatic vessels surround follicles within the ovary, but their roles in folliculogenesis and pregnancy, as well necessity of lymphangiogenesis follicle maturation health, are undefined. We used systemic delivery mF4-31C1, a specific antagonist vascular endothelial growth factor receptor 3 (VEGFR-3) antibody to block mice. VEGFR-3 neutralization for 2 weeks before mating blocked ovarian at all stages maturation, most notably around corpora lutea, without significantly affecting follicular...
Tumor-associated lymphatic vessels actively participate in tumor progression and dissemination. ADAM17, a sheddase for numerous growth factors, cytokines, receptors, cell adhesion molecules, is believed to promote development, facilitating both proliferation migration, as well angiogenesis. In this work we addressed the issue of whether ADAM17 may also lymphangiogenesis. First, found that important migratory potential immortalized human dermal endothelial cells (LEC). When was stably...
Besides being a physical scaffold to maintain tissue morphology, the extracellular matrix (ECM) is actively involved in regulating cell and function during development organ homeostasis. It does so by acting via biochemical, biomechanical, biophysical signaling pathways, such as through release of bioactive ECM protein fragments, tension, providing pathways for migration. The tumor microenvironment undergoes substantial remodeling, characterized degradation, deposition organization fibrillar...
Filariases are diseases caused by arthropod-borne filaria nematodes. The related pathologies depend on the location of infective larvae when their migration, asymptomatic and least studied phase disease, comes to an end. To determine factors assisting in filariae dissemination, we image Litomosoides sigmodontis during escape from skin. Burrowing through dermis exclusively enter pre-collecting lymphatics mechanical disruption wall. Once inside collectors, rapid unidirectional movement towards...