A5091247902- DNA Repair Mechanisms
- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Nuclear Structure and Function
- Genetic factors in colorectal cancer
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Cancer Genomics and Diagnostics
- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- Mitochondrial Function and Pathology
- Malaria Research and Control
- Lipid Membrane Structure and Behavior
- Advanced biosensing and bioanalysis techniques
- RNA Research and Splicing
- Protein Structure and Dynamics
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- Antibiotic Resistance in Bacteria
Institute of Molecular Life Sciences
2014-2022
Budapest University of Technology and Economics
2014-2022
HUN-REN Research Centre for Natural Sciences
2014-2022
Hungarian Research Network
2021
University of Szeged
2014-2020
Hungarian Academy of Sciences
2014-2019
Cancer genome sequencing has implicated the cytosine deaminase activity of apolipoprotein B mRNA editing enzyme catalytic polypeptide-like (APOBEC) genes as an important source mutations in diverse cancers, with APOBEC3B (A3B) expression especially correlated such cancer mutations. To better understand processes directing A3B over-expression cancer, and possible therapeutic avenues for targeting A3B, we have investigated regulation gene expression. Here, show that is inversely related to p53...
Transfer of phage-related pathogenicity islands Staphylococcus aureus (SaPI-s) was recently reported to be activated by helper phage dUTPases. This is a novel function for dUTPases otherwise involved in preservation genomic integrity sanitizing the dNTP pool. Here we investigated molecular mechanism dUTPase-induced gene expression control using direct techniques. The SaPI transfer initiating proteins repressed called Stl. We found that Φ11 dUTPase eliminates SaPIbov1 Stl binding its cognate...
The role of uracil in genomic DNA has been recently re-evaluated. It is now widely accepted to be a physiologically important element diverse systems from specific phages antibody maturation and Drosophila development. Further relevant investigations would largely benefit novel reliable fast method gain quantitative qualitative information on levels both vitro situ, especially since current techniques does not allow situ cellular detection. Here, starting catalytically inactive uracil-DNA...
Sanitization of nucleotide pools is essential for genome maintenance. Deoxyuridine 5′-triphosphate nucleotidohydrolase (dUTPase) a key enzyme in this pathway since it catalyzes the cleavage 2′-deoxyuridine (dUTP) into 5′-monophosphate (dUMP) and inorganic pyrophosphate. Through its action dUTPase efficiently prevents uracil misincorporation DNA at same time provides dUMP, substrate de novo thymidylate biosynthesis. Despite physiological significance, knock-out models have not yet been...
Numerous anti-cancer drugs perturb thymidylate biosynthesis and lead to genomic uracil incorporation contributing their antiproliferative effect. Still, it is not yet characterized if incorporations have any positional preference. Here, we aimed uncover genome-wide alterations in pattern upon drug treatments human cancer cell line models derived from HCT116. We developed a straightforward U-DNA sequencing method (U-DNA-Seq) that was combined with situ super-resolution imaging. Using novel...
Plasmodium falciparum parasites undergo multiple genome duplication events during their development. Within the intraerythrocytic stages, encounter an oxidative environment and DNA synthesis necessarily proceeds under these circumstances. In addition to conditions, extreme AT bias of P. poses further constraints for synthesis. Taken together, circumstances may allow appearance damaged bases in DNA. Here, we focus on uracil that arise either via deamination or thymine-replacing incorporation....
The clonal composition of a malignant tumor strongly depends on cellular dynamics influenced by the asynchronized loss DNA repair mechanisms. Here, our aim was to identify founder mutations leading subsequent boosts in mutation load. overall burden 591 colorectal cancer tumors analyzed, including status DNA-repair genes. number first determined across all patients and proportion genes having each percentile ranked. Early preceding mutational expansion were designated as mutations. Survival...
Nucleocytoplasmic trafficking of large macromolecules requires an active transport machinery. In many cases, this is initiated by binding the nuclear localization signal (NLS) peptide cargo proteins to importin-α molecules. Fine orchestration nucleocytoplasmic particularly high importance for involved in maintenance genome integrity, such as dUTPases, which are responsible prevention uracil incorporation into genome. most eukaryotes, dUTPases have two homotrimeric isoforms: one these...
Abstract The phospholipid biosynthesis of the malaria parasite, Plasmodium falciparum is a key process for its survival and inhibition validated antimalarial therapeutic approach. second rate-limiting step de novo phosphatidylcholine catalysed by CTP: phosphocholine cytidylyltransferase ( Pf CCT), which has regulatory function within pathway. Here, we investigate functional impact structural differences their respective role in structurally unique pseudo-heterodimer CCT protein heterologous...
Abstract Sanitization of nucleotide pools is essential for genome maintenance. Among the enzymes significant in this mechanism, deoxyuridine 5′-triphosphate nucleotidohydrolase (dUTPase) performs cleavage dUTP into dUMP and inorganic pyrophosphate. By reaction enzyme efficiently prevents uracil incorporation DNA provides dUMP, substrate de novo thymidylate biosynthesis. Despite its physiological significance, knock-out models dUTPase have not yet been investigated mammals, only unicellular...
ABSTRACT Numerous anti-cancer drugs perturb thymidylate biosynthesis and lead to genomic uracil incorporation contributing their antiproliferative effect. Still, it is not yet characterized if incorporations have any positional preference. Here, we aimed uncover genome-wide alterations in pattern upon drug-treatment human cancer cell-line HCT116. We developed a straightforward U-DNA sequencing method (U-DNA-Seq) that was combined with situ super-resolution imaging. Using novel robust...