A5044263958- Cancer, Hypoxia, and Metabolism
- Glioma Diagnosis and Treatment
- Medical Imaging Techniques and Applications
- Brain Metastases and Treatment
- Erythropoietin and Anemia Treatment
- Mitochondrial Function and Pathology
- Nanoplatforms for cancer theranostics
- Radiopharmaceutical Chemistry and Applications
- Radiation Therapy and Dosimetry
- Immune cells in cancer
- Advanced MRI Techniques and Applications
- Effects of Radiation Exposure
- Melanoma and MAPK Pathways
- Traumatic Brain Injury and Neurovascular Disturbances
- MRI in cancer diagnosis
- Neuroscience and Neuropharmacology Research
- Radiomics and Machine Learning in Medical Imaging
- Neuroinflammation and Neurodegeneration Mechanisms
- Angiogenesis and VEGF in Cancer
- MicroRNA in disease regulation
- Nanoparticle-Based Drug Delivery
- Neurological Disorders and Treatments
- Brain Tumor Detection and Classification
- Neurological Disease Mechanisms and Treatments
- Adipose Tissue and Metabolism
Université de Caen Normandie
2014-2025
Centre National de la Recherche Scientifique
2014-2025
Normandie Université
2014-2025
Imagerie et Stratégies Thérapeutiques pour les Cancers et Tissus cérébraux
2022-2024
Cyceron
2015-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2012-2021
Mécanismes Centraux et Périphériques de la Neurodégénérescence
2006-2017
Institut d'Imagerie Biomédicale
2015
Université Claude Bernard Lyon 1
2015
Laboratoire National Henri Becquerel
2000-2014
The present study describes, for the first time, a temporal and spatial cellular expression of erythropoietin (Epo) Epo receptor (Epo-R) with evolution cerebral infarct after focal permanent ischemia in mice. In addition to basal neurons astrocytes, postischemic has been localized specifically endothelial cells (1 day), microglia/macrophage-like (3 days), reactive astrocytes (7 days occlusion). Under these conditions, Epo-R always precedes that each cell type. These results support...
Tolerance to cerebral ischemia is achieved by preconditioning sublethal stresses, such as or hypoxia, paradigms in which the decrease of O 2 availability may constitute an early signal inducing tolerance. In accordance with this concept, study shows that hypoxia induces tolerance against focal permanent adult mice. Normobaric (8% 1-hour, 3-hour, 6-hour duration), performed 24 hours before ischemia, reduces infarct volume approximately 30% when compared controls. To elucidate mechanisms...
Hypoxic preconditioning (8% O2, 3 h) produces tolerance 24 h after hypoxic-ischemic brain injury in neonatal rats. To better understand the ischemic mechanisms induced by hypoxia, we used oligonucleotide microarrays to examine genomic responses rat following of hypoxia O2) and either 0, 6, 18, or re-oxygenation. The results showed that hypoxia-inducible factor (HIF)-1- but not HIF-2-mediated gene expression may be involved hypoxia-induced tolerance. Among genes regulated 12 were confirmed...
Erythropoietin (Epo), the major hormone controlling hypoxia-induced increase in number of erythrocytes, has also a functional role brain. However, few data exist as to cellular source brain-derived Epo well molecular mechanisms that control expression central nervous system. Using patch-clamp and RT-PCR methods, we provide direct evidence that, besides astrocytes, neurons are Both astrocytic neuronal mRNA induced not only by hypoxia, but desferrioxamine (DFX) cobalt chloride (CoCl(2)), two...
After cerebral ischemia, angiogenesis, by supplying for the deficient perfusion, may be a beneficial process limiting neuronal death and promoting tissue repair. In this study, we showed that combination of Ang-1 vascular endothelial growth factor (VEGF) provides more adapted therapeutic strategy than use VEGF alone. Indeed, on focal ischemia model an early administration exacerbates ischemic damage, because its effects blood-brain barrier (BBB) permeability. contrast, coapplication leads to...
Cancer cells can undergo a metabolic reprogramming from oxidative phosphorylation to glycolysis that allows them adapt nutrient-poor microenvironments, thereby imposing selection for aggressive variants. However, the mechanisms underlying this are not fully understood. Using complementary approaches in validated cell lines and freshly obtained human specimens, we report here mitochondrial respiration slowed metastatic melanomas, even under normoxic conditions due persistence of high nuclear...
// Marine M. Leblond 1 , Elodie A. Pérès Charly Helaine Aurélie N. Gérault Damien Moulin Clément Anfray Didier Divoux Edwige Petit Myriam Bernaudin and Samuel Valable Normandie Université, UNICAEN, CEA, CNRS, ISTCT/CERVOxy Group, 14000 Caen, France Correspondence to: Valable, email: valable@cyceron.fr Keywords: macrophages, irradiation, glioblastoma, radioresistance, phenotypic selection Received: December 21, 2016 Accepted: July 23, 2017 Published: August 07, ABSTRACT In some highly...
Hypoxia is a common feature of solid tumors, particularly in glioblastoma (GBM), and known to be poor prognosis factor GBM patients. The growth also associated with marked inflammation partially characterized by an accumulation macrophage (MΦ) the M2 phenotype. However, transition between M1 MΦ (antitumoral) (protumoral) phenotypes dynamic process. We made assumption that oxygen (O2) availability could major regulator this intratumoral O2 gradient importance. evaluated, vivo, impact hypoxia...
Erythropoietin, originally defined as an erythroid growth factor, is upregulated in the brain under conditions of hypoxia. So far, two functions have been identified for this locally produced cytokine: a direct protective effect on neuronal cells during cerebral ischemia and indirect protection tissue that could be provided by promoting vessel growth.
Bone-marrow-derived mesenchymal stem cells (MSCs) constitute an interesting cellular source to promote brain regeneration after neurodegenerative diseases. Recently, several studies suggested that oxygen-dependent gene expression is of crucial importance in governing the essential steps neurogenesis such as cell proliferation, survival and differentiation. In this context, we analysed effect HIF-1 (hypoxia inducible factor-1) activation-mimicking agent CoCl(2) on MSCs. treatment increased...
Abstract The mechanisms underlying functional recovery after stroke are poorly understood. Brain‐adaptive responses to the hypoxic stress elicited by ischemia could contribute these mechanisms. Indeed, hypoxia‐inducible factor‐1 (HIF‐1), one of main transcriptional factors regulated oxygen level, increases expression several beneficial genes such as erythropoietin, glucose transporter‐1 and vascular endothelial growth factor. In order strengthen factors, we administered deferoxamine, an iron...
Background and Purpose— Inspired from preconditioning studies, ischemic postconditioning, consisting of the application intermittent interruptions blood flow shortly after reperfusion, has been described in cardiac ischemia recently stroke. It is well known that tolerance can be achieved brain not only by preconditioning, but also hypoxic preconditioning. However, existence postconditioning never reported cerebral ischemia. Methods— Adult mice subjected to transient middle artery occlusion...
Abstract BACKGROUND: O 6 ‐methylguanine‐DNA methyltransferase (MGMT) promoter methylation status was proposed as a prognostic biomarker for patients with glioblastoma. However, the impact of MGMT in newly diagnosed glioblastoma who receive carmustine‐releasing wafers (Gliadel) along temozolomide (TMZ) is still unknown. METHODS: and protein expression were analyzed formalin‐fixed, paraffin‐embedded tumor specimens obtained from 111 French Patients received Gliadel followed by radiotherapy...