A5038674624- Lipid Membrane Structure and Behavior
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Neuroscience and Neuropharmacology Research
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Bacterial Genetics and Biotechnology
- Receptor Mechanisms and Signaling
- T-cell and B-cell Immunology
- Supramolecular Self-Assembly in Materials
- Immune Cell Function and Interaction
- Bacteriophages and microbial interactions
- Chemical Synthesis and Analysis
- Antibiotic Resistance in Bacteria
- Caveolin-1 and cellular processes
- Cellular transport and secretion
- Polysaccharides and Plant Cell Walls
- Cell Adhesion Molecules Research
- Systemic Lupus Erythematosus Research
- RNA and protein synthesis mechanisms
- NF-κB Signaling Pathways
- Advanced biosensing and bioanalysis techniques
- Graphene and Nanomaterials Applications
- Cellular Mechanics and Interactions
- RNA Research and Splicing
Université de Bordeaux
2012-2023
Institut Polytechnique de Bordeaux
2014-2023
Institut Européen de Chimie et Biologie
2016-2023
Chimie et Biologie des Membranes et des Nanoobjects
2015-2023
Centre National de la Recherche Scientifique
2012-2022
Institut de Biologie Moléculaire et Cellulaire
2008-2017
Immunologie, Immunopathologie et Chimie Thérapeutique
2006-2017
Université de Strasbourg
2011-2014
Bordeaux Imaging Center
2012
Macroautophagy was recently shown to regulate both lymphocyte biology and innate immunity. In this study we sought determine whether a deregulation of autophagy linked the development autoimmunity. Genome-wide association studies have pointed out nucleotide polymorphisms that can be associated with systemic lupus erythematosus, but potential role in initiation and/or syndrome is still unknown. Here, provide first clues macroautophagy lupus. By use LC3 conversion assays electron microscopy...
Fraternal twins: Oligoureas and γ-peptides are isosteric, quasi-isostructural helical foldamers endowed with distinct biomolecular recognition properties. Combination of the two backbones to generate urea/amide hybrids (see picture) was found give more potent yet less cytotoxic antimicrobial foldamers.
Perovskite nanocrystals (PNCs) exhibit excellent absorption and luminescent properties. Inorganic silica right (or left) handed nanohelices are used as chiral templates to induce optically active properties CsPbBr3 PNCs grafted on their surfaces. In suspension, the do not show any detectable chiroptical contrast, in a dried film state, they large circular dichroism (CD) circularly polarized luminescence (CPL) signals with dissymmetric factor up 6 × 10-3. Grazing incidence X-ray scattering,...
<h3>Background</h3> The P140 phosphopeptide issued from the spliceosomal U1-70K small nuclear ribonucleoprotein protein displays protective properties in MRL/lpr lupus-prone mice. It binds both major histocompatibility class II (MHCII) and HSC70/Hsp73 molecules. peptide increases peripheral blood lymphocyte apoptosis decreases autoepitope recognition by T cells. <h3>Objective</h3> To explore further mode of action on HSC70+ antigen-presenting <h3>Methods</h3> biodistribution was monitored...
Abstract Despite significant advances in foldamer chemistry, tailored delivery systems based on architectures, which provide a high level of control over secondary structure, are curiously rare among non‐viral technologies for transporting nucleic acids into cells. A potent pH‐responsive, bioreducible cell‐penetrating (CPF) was developed through covalent dimerization short (8‐mer) amphipathic oligourea sequence bearing histidine‐type units. This CPF exhibits capacity to assemble with pDNA...
Abstract The tripartite multidrug efflux system MexAB-OprM is a major actor in Pseudomonas aeruginosa antibiotic resistance by exporting large variety of antimicrobial compounds. Crystal structures MexB and its Escherichia coli homolog AcrB had revealed asymmetric trimers depicting directional drug pathway conformational interconversion (from Loose Tight binding pockets to Open gate (LTO) for exit). It remains unclear how acquires LTO form. Here performing functional cryo-EM structural...
Receptor activator of NF-κB (RANK), known for controlling bone mass, has been recognized its role in epithelial cell activation the mammary gland. Because and epidermo-pilosebaceous unit skin share a lifelong renewal activity where similar molecular players operate, because glands hair follicles are both appendages, we have addressed function RANK follicle epidermis. Here, show that mice deficient ligand (RANKL) unable to initiate new growth phase cycle display arrested epidermal...
Abstract Nanodiscs offer a very promising tool to incorporate membrane proteins into native‐like lipid bilayers and an alternative liposomes maintain protein functions protein–lipid interactions in soluble nanoscale object. The activity of the incorporated appears be correlated its dynamics bilayer by interactions. These two parameters depend on internal surrounded lipid‐encircling discoidal scaffold that might differ from more unrestricted observed vesicles or cellular extracts. A...
An immunodetection study of protein tyrosine phosphatase 1B (PTP-1B), SHP-2, and Src in isolated mitochondria from different rat tissues (brain, muscle, heart, liver, kidney) revealed their exclusive localization the brain. Given this result, we sought whether respond to ATP general inhibitor orthovanadate found little or no change phosphorylation profile kidney. In contrast, induced an enhancement tyrosine-phosphorylated brain mitochondria, which was further greatly enhanced with...
The phosphopeptide P140 issued from the spliceosomal U1-70K snRNP protein is recognized by lupus CD4+ T cells, transiently abolishes cell reactivity to other peptides in P140-treated MRL/lpr mice, and ameliorates their clinical features. modulates patients' response ex vivo currently included phase IIb trials. Its underlying mechanism of action remains elusive. Here we show that peptide binds a unique cell-surface receptor, constitutively-expressed chaperone HSC70 protein, known as...
Humoral responses are central to the development of chronic autoimmune diseases such as systemic lupus erythematosus. Indeed, autoantibody deposition is responsible for tissue damage, kidneys being one main target organs. As source pathogenic antibodies, plasma cells therefore critical players in this harmful scenario, both at and local levels. The aim present study was analyze NZB/W mice get a better understanding mechanisms underlying their involvement renal inflammation process. Using...
Abstract RANK and its ligand RANKL play important roles in the development regulation of immune system. We show that mice transgenic for Rank hair follicles display massive postnatal growth skin-draining lymph nodes. The proportions hematopoietic nonhematopoietic stromal cells their organization are maintained, with exception an increase B cell follicles. not activated respond to immunization by foreign Ag adjuvant. demonstrate soluble is overproduced from neutralization normalizes node...
The toxicity of amyloids, as Aβ(1-42) involved in Alzheimer disease, is a subject under intense scrutiny. Many studies link their to the existence various intermediate structures prior fiber formation and/or specific interaction with membranes. In this study we focused on between membrane models and peptides variants (L34T, mG37C) produced E. coli purified monomeric form. We evaluated toxic stable oligomeric form (oG37C) membranes comparison. Using biophysical techniques fluorescence plasmon...
The plant plasma membrane (PM) is an essential barrier between the cell and external environment, controlling signal perception transmission. It consists of asymmetrical lipid bilayer made up three different classes: sphingolipids, sterols, phospholipids. glycosyl inositol phosphoryl ceramides (GIPCs), representing to 40% total are assumed be almost exclusively in outer leaflet PM. However, their biological role properties poorly defined. In this study, we investigated GIPCs organization....
Abstract Objective Naturally occurring CD4+,CD25+ Treg cells are central in the maintenance of peripheral tolerance. Impaired activity and/or a lower frequency these is involved emergence autoimmunity. We undertook this study to analyze relative proportions and functional alterations MRL/ lpr mice. Methods The T blood healthy autoimmune mice was compared by flow cytometry. capacity inhibit proliferation cytokine secretion CD4+,CD25− assessed after polyclonal activation. Results exhibited...
Functionalized carbon nanohorns (f-CNH) are uptaken by macrophages, without affecting cell viability. f-CNH induce the production of reactive oxygen species and pro-inflammatory cytokines. The level inflammation, although moderate, should be taken into consideration when using for drug delivery. However, it could exploited as an intrinsic adjuvant function biomedical applications requiring some activation immune system. Supporting information this article is available on WWW under...