Dengue fever remains a significant public health concern in tropical regions, including Central India, where outbreaks are frequent and associated with high morbidity mortality. This study investigated the dynamics of dengue virus transmission evolution India from 2019 to 2023, focusing on emergence new strains their impact outbreak patterns. For this, 40 mosquito pools 300 patient samples were recruited for study. Phylogenetic Bayesian evolutionary analyses performed CPrM region whole...

Phylogenetic analyses can give new insights into the evolutionary history of viruses, especially viruses with segmented genomes. However, sequence information for many viral families or genera is still limited and phylogenies based on single short genome fragments be misleading. We report first genetic analysis all three segments Wyeomyia group Wyeomyia, Taiassui, Macaua, Sororoca, Anhembi Cachoeira Porteira (BeAr328208) in genus Orthobunyavirus family Bunyaviridae . In addition, Tucunduba...

Summary Almost all current genetically modified plant commercial products are derived from seeds. The first protein product made in leaves for use is reported here. Leaf pectinases validated here with eight liquid microbial enzyme textile or juice industry applications. functional broad pH /temperature ranges as crude leaf extracts, while most showed significant loss at alkaline higher temperature, essential various In contrast to enzymes requiring cold storage/transportation, pectinase...

Aim: This study is an overview of non‐polio enterovirus (NPEV) circulating in North India studied from the perspective poliomyelitis eradication. Wild polio cases declined because intensive oral vaccine immunization. As we approach global eradication poliovirus (PV), NPEV causing acute flaccid paralysis (AFP) are equal cause concern. Methods: A total 46 653 AFP samples (World Health Organization) and apparently 1000 healthy contacts living same geographical area were (2004–2007). Serological...

ABSTRACT There is a need in clinical microbiology and public health for assays that enable sensitive rapid detection of infectious agents. Next Generation Sequencing (NGS) increasingly used the fields oncology personalized genome medicine but has not gained wider acceptance due to operational bioinformatics complexity, as well lower sensitivity compared agent-specific quantitative polymerase chain reaction (qPCR) assays. VirCapSeq-VERT positive selection system detection, typing, strain...

Objectives Restless Leg Syndrome (RLS) is a neurological disorder characterized by an uncontrollable urge to move the legs, often worsening at night, leading insomnia and discomfort. RLS linked genetic factors, defects in iron metabolism, dopaminergic dysfunction, disturbances central opiate system. Specific variants, including MEIS1, BTBD9, PTPRD, MAP2K5/SCOR1, affect dopamine synthesis, transport, neuroprotection. Research on prevalence its underlying causes India limited, highlighting...

Background: Respiratory droplets are the main way in which COVID-19 pandemic’s causal agent, severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), spreads. Angiotensin-converting enzyme 2 (ACE2) receptors, especially lung cells, allow virus to enter host cells. However, ACE2 expression intestinal cells has sparked worries about possible fecal transfer, particularly poor-sanitation areas like India. Methods: Between July 2021 and 2024, clinical (nasopharyngeal, saliva, stool samples)...

ABSTRACT Emerging and zoonotic pathogens pose continuing threats to human health ongoing challenges diagnostics. As nucleic acid tests are playing increasingly prominent roles in diagnostics, the genetic characterization of molecularly uncharacterized agents is expected significantly enhance detection surveillance capabilities. We report identification two previously unrecognized members family Orthomyxoviridae , which includes influenza viruses tick-transmitted Thogoto Dhori viruses....

This study is evaluating CD146 expression on a set of gynecological cancers and site-specific controls.CD146 IHC immunoreactivity score, microvessel density caliber are evaluated.

Despite polio eradication, nonpolio enterovirus (NPEV) detection amid surveillance, which is considered to have implications in paralysis, requires attention. The attributes of NPEV infections nonpolio-AFP (NPAFP) cases from Uttar Pradesh (UP), India, remain undetermined and are thus investigated. A total 1839 stool samples collected patients with acute flaccid paralysis (AFP) UP, between January 2010 October 2011 were analyzed as per the WHO algorithm. 359 NPAFP yielded NPEVs, subjected...

Abstract Global eradication of poliomyelitis has reached critical stage. Sabin Oral Poliovirus Vaccine (OPV) been successful in three major regions the world. In India from states Uttar Pradesh (UP) and Bihar difficult due to high population low‐socioeconomic standards living. Acute flaccid paralysis (AFP) surveillance intensive OPV rounds continues with World Health Organization (WHO) operational strategies. Yet apparent lack progress reducing number wild cases resulted occasional...

Nonpolio acute flaccid paralysis is increasing in India. To determine viral causes, we conducted cell culture and molecular analysis identification of nonpolio human enteroviruses associated with during March-August 2010 northern The predominant enterovirus found was echovirus 13, a serotype rarely isolated

The Mapputta group comprises antigenically related viruses indigenous to Australia and Papua New Guinea that are included in the family Bunyaviridae but not currently assigned a specific genus. We determined analyzed genome sequences of five Australian isolated from mosquitoes collected during routine arbovirus surveillance Western (K10441, SW27571, K13190, K42904) South Wales (12005). Based on matching all three segments prototype MRM3630 Trubanaman virus (TRUV), NB6057 Gan (GGV), MK7532...

Background: Wound healing, a complex biological process, is crucial for preventing infections and maintaining skin integrity.This comprehensive review examines wound dynamics based on location, etiology, symptoms, clinical appearance.Emphasizing the importance of precise progression through inflammation, cellular proliferation, remodeling phases, study highlights risk chronic wounds with delays or interruptions.Methods: This explores natural alternatives to synthetic healing drugs, focusing...

Background: Head neck squamous cell carcinoma (HNSC) is globally prevalent cancer attributed to tobacco habit. Despite the significant advances in early diagnosis and treatment of HNSC chemo-radio resistance are routinely observed patients. Aberrant DNA repair mechanisms mainly microhomology mediated end joining (MMEJ) pathway causing deleterious mutations implicated resistance. X-ray cross complimenting group 1 (XRCC1) has recently been shown play an essential role MMEJ making XRCC1 a...

The authors' objective in this study was to introduce and evaluate integrated cell culture polymerase chain reaction (ICC-PCR) as a technique for the rapid screening of poliovirus sewage samples. Researchers are last stage poliomyelitis eradication; however, densely populated country such India, time is prime factor identification circulation transmission because virus follows fecal-oral route excreted nature. authors used ICC-PCR detect samples, they compared nonconventional method with...

The University Medical Center's Emergency Department (ED) in Tucson, Arizona, has faced continually increasing patient numbers and mounting costs while resources have remained fairly static. This phenomenon, termed "crowding" been observed as a growing problem nationwide. purpose of this project is to decrease overall throughput time by identifying potential bottlenecks proposing strategies alleviate them. A simulation model the ED process created using ARENA software. reflects data obtained...

Influenza A/H1N1pdm09 causes respiratory illness and remains a concern for public health. Since its first emergence in 2009, the virus has been continuously circulating form of genetic variants. surveillance is essential uncovering emerging variants epidemiologic vaccine efficacy. The present study attempts silico analysis molecular characterization A (H1N1) pdm09 causing major outbreaks central India during 2009-2019.We have investigated antigenic drift 96 isolates' hemagglutinin (HA) gene...