A5081487910- Enzyme Catalysis and Immobilization
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Carbohydrate Chemistry and Synthesis
- Biochemical and Molecular Research
- Proteins in Food Systems
- Antimicrobial Peptides and Activities
- Pharmacology and Obesity Treatment
- Lipid Membrane Structure and Behavior
- Synthesis and biological activity
- Lipid metabolism and biosynthesis
- Pancreatic function and diabetes
- Analytical Chemistry and Chromatography
- Diabetes Management and Research
- Synthesis and Characterization of Heterocyclic Compounds
- Bacteriophages and microbial interactions
- HIV/AIDS drug development and treatment
- Synthesis and Biological Evaluation
- Antibiotic Resistance in Bacteria
- Microbial Metabolic Engineering and Bioproduction
- Pneumocystis jirovecii pneumonia detection and treatment
- Microbial Natural Products and Biosynthesis
- Synthesis of Organic Compounds
- Click Chemistry and Applications
- Organophosphorus compounds synthesis
Aix-Marseille Université
2016-2025
Centre National de la Recherche Scientifique
2015-2025
Institut de Microbiologie de la Méditerranée
2018-2025
Laboratoire d'Ingénierie des Systèmes Macromoléculaires
1999-2024
Institute of Molecular Life Sciences
2015-2016
Laboratoire Science & technologie du Lait & de l'œuf
2016
UCLouvain
2001-2003
Catalyse
1998-1999
Laboratoire de Synthèse Organique
1999
Institut National de la Transfusion Sanguine
1981-1985
Abstract Background Mycobacterium abscessus , a fast-growing non-tuberculous mycobacterium, is an emerging opportunistic pathogen responsible for chronic bronchopulmonary infections in people with respiratory diseases such as cystic fibrosis (CF). Due to its intrinsic polyresistance wide range of antibiotics, most treatments M. pulmonary are poorly effective. In this context, antimicrobial peptides (AMPs) active against bacterial strains and less prompt cause resistance, represent good...
During infection and granuloma formation, pathogenic mycobacteria store triacylglycerol as intrabacterial lipid inclusions (ILIs). This accumulation of nutrients provides a carbon source for bacterial persistence slows down intracellular metabolism. Mycobacterium abscessus (Mab), rapidly growing non-tuberculous actinobacterium, produces ILI throughout its cycle. Here, Mab was used model organism to identify proteins associated with on global scale. By using the APEX2 proximity labeling...
Tuberculosis caused by Mycobacterium tuberculosis is currently one of the leading causes death from an infectious agent. The main difficulties encountered in eradicating this bacteria are mainly related to (i) a very complex lipid composition bacillus cell wall, (ii) its ability hide immune system inside granulomas, and (iii) increasing number resistant strains. In context, we were interested Rv0646c (lipGMTB ) gene located upstream mmaA cluster which described as being crucial for...
Mycobacteria share with other actinomycetes the ability to produce large quantities of triacylglycerol (TAG), which accumulate as intracytoplasmic lipid inclusions (ILI) also known droplets (LD). Mycobacterium tuberculosis (M. tb), etiologic agent tuberculosis, acquires fatty acids from human host are utilized synthesize TAG, subsequently stored in form ILI meet carbon and nutrient requirements bacterium during long periods persistence. However, environmental factors governing mycobacterial...
Lipid metabolism plays an important role during the lifetime of Mycobacterium tuberculosis, causative agent tuberculosis. Although M. tuberculosis possesses numerous lipolytic enzymes, very few have been characterized yet at a biochemical/pharmacological level. This study was devoted to enzymes belonging Hormone-Sensitive Lipase (HSL) family, which encompasses twelve serine hydrolases closely related human HSL. Among them, nine were expressed, purified and biochemically using broad range...
A new series of customizable diastereomeric cis- and trans-monocyclic enol-phosphonate analogs to Cyclophostin Cyclipostins were synthesized. Their potencies mechanisms inhibition toward six representative lipolytic enzymes belonging distinct lipase families examined. With mammalian gastric pancreatic lipases no occurred with any the compounds tested. Conversely, Fusarium solani Cutinase from Mycobacterium tuberculosis (Rv0183 LipY) all fully inactivated. The best inhibitors displayed a cis...
Abstract A new class of Cyclophostin and Cyclipostins ( CyC ) analogs have been investigated against Mycobacterium tuberculosis H37Rv M. tb grown either in broth medium or inside macrophages. Our compounds displayed a diversity action by acting on extracellular bacterial growth only, both intracellularly infected macrophages as well extracellularly with very low toxicity towards host Among the eight potential CyCs identified, 17 exhibited best antitubercular activity (MIC 50 = 500 nM). This...
An increasing prevalence of cases drug-resistant tuberculosis requires the development more efficacious chemotherapies. We previously reported discovery a new class cyclipostins and cyclophostin (CyC) analogs exhibiting potent activity against Mycobacterium both in vitro infected macrophages. Competitive labeling/enrichment assays combined with MS have identified several serine or cysteine enzymes lipid cell wall metabolism as putative targets these CyC compounds. These included members...
Mycolactone is a lipid-like endotoxin synthesized by an environmental human pathogen, Mycobacterium ulcerans, the causal agent of Buruli ulcer disease. has pleiotropic effects on fundamental cellular processes (cell adhesion, cell death and inflammation). Various targets mycolactone have been identified literature survey revealed that most these are membrane receptors residing in ordered plasma nanodomains, within which their functionalities can be modulated. We investigated capacity to...
Twelve new Cyclophostin and Cyclipostins analogues (CyC19-30) were synthesized, thus extending our series to 38 CyCs. Their antibacterial activities evaluated against four pathogenic mycobacteria (Mycobacterium abscessus, Mycobacterium marinum, bovis BCG, tuberculosis) two Gram negative bacteria. The CyCs displayed very low toxicity toward host cells only active mycobacteria. Importantly, several extracellular M. abscessus (CyC17/CyC18β/CyC25/CyC26) or intramacrophage residing...
The constant emergence of drug-resistant mycobacteria, together with the lack new antibiotics entering market, has become a global public health problem that threatens effective treatment infectious diseases. development single molecules targeting different proteins should significantly reduce resistant strains, and therefore represent promising strategy to overcome such an issue. In this challenging context, series 30 lipophilic compounds based on β-lactone-core been synthesized by varying...
Lipids play a critical role in the physiology, life cycle, and pathogenicity of mycobacteria. They largely participate host-pathogen interactions fulfill important functions ranging from cell wall biosynthesis/maintenance, bacterial growth dynamics, long-term persistence. In that context, triacylglycerol, specific subtype neutral lipid, is stored as intrabacterial lipid inclusions (ILI), which have been described structures for survival persistence within host. However, enzymes involved ILI...