A5063947250- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Acute Lymphoblastic Leukemia research
- Acute Myeloid Leukemia Research
- Sarcoidosis and Beryllium Toxicity Research
- Neuroblastoma Research and Treatments
- Chemical Synthesis and Analysis
- Congenital Diaphragmatic Hernia Studies
- Lymphoma Diagnosis and Treatment
- Sarcoma Diagnosis and Treatment
- Congenital Anomalies and Fetal Surgery
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- Nitric Oxide and Endothelin Effects
- Hematopoietic Stem Cell Transplantation
- Cancer, Hypoxia, and Metabolism
- Blood Coagulation and Thrombosis Mechanisms
- Flow Measurement and Analysis
- Cardiac Structural Anomalies and Repair
- Multiple Myeloma Research and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Medical Imaging and Pathology Studies
- Asthma and respiratory diseases
- Amyloidosis: Diagnosis, Treatment, Outcomes
The University of Tokyo
2014-2025
Kurashiki Central Hospital
2024
University of Tokyo Hospital
2024
Kyoto University
1989-2023
Stem Cell Institute
2023
Wellcome/MRC Cambridge Stem Cell Institute
2023
Saiseikai Central Hospital
2002-2021
Fukuoka Hospital
2020-2021
Nihon University
2021
Nagoya Ekisaikai Hospital
2021
Cultured human umbilical vein endothelial cells (HUVEC) stimulated with thrombin are known to synthesize prostacyclin at least in part from arachidonate released by phospholipase A2, an enzyme directly activated calcium. In this study, stimulation of Quin 2-loaded HUVEC caused rapid and dose-dependent rises inositol trisphosphate (IP3) cytosolic free calcium (Ca2+i) levels which preceded a similarly rise production measured as 6-ketoprostaglandin F1 alpha (6-keto-PGF1 alpha) radioimmunoassay...
The α-helix structures of the anti-HIV fusion inhibitory peptides are stabilized by amino acid sequence and intrachain hydrogen bonds. study peptide analogues using (E)-alkene (Z)-fluoroalkene dipeptide isosteres demonstrated substantial, yet position-dependent, contribution bonds to stability bioactivity.
ABSTRACT Peptides derived from the α-helical domains of human immunodeficiency virus (HIV) type 1 (HIV-1) gp41 inhibit HIV-1 fusion to cell membrane. Enfuvirtide (T-20) is a peptide-based drug that targets step HIV fusion, and as such, it effectively suppresses replication strains are either wild or resistant multiple reverse transcriptase and/or protease inhibitors. However, variants with T-20 resistance have emerged; therefore, development new potent inhibitors urgently needed. We...
Abstract Although hepatoblastoma is the most common pediatric liver cancer, its genetic heterogeneity and therapeutic targets are not well elucidated. Therefore, we conducted a multiomics analysis, including mutatome, DNA methylome, transcriptome analyses, of 59 samples. Based on methylation patterns, was classified into three clusters exhibiting remarkable correlation with clinical, histological, features. Cluster F largely composed cases fetal histology good outcomes, whereas E1 E2...
KMT2A-rearranged infant acute lymphoblastic leukemia (ALL) represents the most refractory type of childhood leukemia. To uncover molecular heterogeneity this disease, we perform RNA sequencing, methylation array analysis, whole exome and targeted deep sequencing on 84 infants with Our multi-omics clustering followed by single-sample single-cell inference hematopoietic differentiation establishes five robust integrative clusters (ICs) different master transcription factors, fusion partners...
Abstract Novel therapeutic strategies are urgently required for osteosarcoma, given the early age at onset and persistently high mortality rate. Modern transcriptomics techniques can identify differentially expressed genes (DEGs) that may serve as biomarkers targets, so we screened DEGs in osteosarcoma. We found osteosarcoma cases could be divided into fair poor survival groups based on gene expression profiles. Among upregulated group, siRNA-mediated knockdown of glycosylation-related...
Reported herein are the design, biological activities, and biophysical properties of a novel HIV-1 membrane fusion inhibitor. α-Helix-inducible X-EE-XX-KK motifs were applied to design an enfuvirtide analogue 2 that exhibited highly potent anti-HIV activity against wild-type HIV-1, enfuvirtide-resistant strains, HIV-2 strain in vitro. Indispensable residues for bioactivity enfuvirtide, including interacting with N-terminal heptad repeat C-terminal hydrophobic residues, identified.
Enfuvirtide (T-20) is a fusion inhibitor that suppresses replication of human immunodeficiency virus (HIV) variants with multi-drug resistance to reverse transcriptase and protease inhibitors. It peptide derived from the C-terminal heptad repeat (C-HR) HIV-1 gp41, it prevents interactions between C-HR N-terminal HR (N-HR) thus interfering conformational changes are required for viral fusion. However, prolonged therapies T-20 result in emergence T-20-resistant strains contain primary...
Abstract Neuroblastomas require novel therapies that are based on the exploitation of their biological mechanism. To address this need, we analyzed DNA methylation and expression datasets neuroblastomas, extracted a candidate gene characterizing aggressive features, conducted functional studies. Based data, identified subgroup neuroblastoma cases with 11q loss heterozygosity extremely poor prognosis. PHGDH , serine metabolism-related gene, was as strong characteristic in well MYCN...
An underestimated side effect of rituximab is late-onset neutropenia (R-LON), which often resolves spontaneously and rarely results in a severe infection. We herein report case febrile due to R-LON 91-year-old woman with renal failure who was treated induce remission MPO-ANCA-associated vasculitis. Fifty-four days after the last administration, patient hospitalized for R-LON, improved granulocyte colony-stimulating factor antibiotics. Although may resolve remain unnoticed, it can cause...
Abstract Angiotensin converting enzyme (ACE) is present on endothelial cells and plays a role in regulating blood pressure vivo by angiotensin I to II metabolizing bradykinin. Since ACE activity decreased sepsis, the ability of lipopolysaccharide (LPS) suppress cell was tested culturing human umbilical vein (HUVEC) for 0–72 hr with or without LPS then measuring activity. intact HUVEC monolayers incubated (10 μg/ml) markedly time inhibited 33%, 71%, 76% after 24 hr, 48 72 respectively, when...
Hypertension (HT) is a common complication in patients with chronic kidney disease (CKD). However, the relationship between circadian rhythm disorder of blood pressure (BP) and intra-renal damage remains unclear.Ninety glomerular (CGD) were included present study. On basis clinic BP (CBP) 24 h-ambulatory (ABP) measurements, divided into following groups; normotension (NT), white coat HT (WHT), masked (MHT), sustained (SHT). For renal histopathological assessment, we evaluated each biopsy...