About
Contact & Profiles
Research Areas
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Advanced Biosensing Techniques and Applications
- Biosensors and Analytical Detection
- Metabolomics and Mass Spectrometry Studies
Peruvian University of Applied Sciences
2021
Here, we describe a detailed step-by-step protocol for the expression, purification, quantification, and activity determination of key enzymes molecular detection pathogens. Based on previous reports, optimized LbCas12a, Taq DNA polymerase, M-MLV reverse transcriptase, TEV protease to make it compatible with minimal laboratory equipment, broadly available in low- middle-income countries. The produced this have been successfully used applications. For complete details use execution protocol,...
10.1016/j.xpro.2021.100899
article
EN
cc-by-nc-nd
STAR Protocols
2021-11-01
10.17632/wynyf35xf3.1
article
EN
2021-09-23
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