Lesia Tello

ORCID: 0000-0003-0871-4297
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About
Contact & Profiles
Research Areas
  • Advanced biosensing and bioanalysis techniques
  • CRISPR and Genetic Engineering
  • Advanced Biosensing Techniques and Applications
  • Biosensors and Analytical Detection
  • Metabolomics and Mass Spectrometry Studies

Peruvian University of Applied Sciences
2021

Here, we describe a detailed step-by-step protocol for the expression, purification, quantification, and activity determination of key enzymes molecular detection pathogens. Based on previous reports, optimized LbCas12a, Taq DNA polymerase, M-MLV reverse transcriptase, TEV protease to make it compatible with minimal laboratory equipment, broadly available in low- middle-income countries. The produced this have been successfully used applications. For complete details use execution protocol,...

10.1016/j.xpro.2021.100899 article EN cc-by-nc-nd STAR Protocols 2021-11-01
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